Mouse pIgR Antibody

Catalog #: AF2800
15 Citations Datasheet / COA / SDS

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AF2800
AF2800-SP

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Detection of Mouse pIgR by Western Blot.

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Product Details

Citations (15)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse pIgR Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse pIgR
Lys19-Lys645
Accession # O70570

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Blockade of Receptor-ligand Interaction

In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of mouse IgM to immobilized Recombinant Mouse pIgR (Catalog # 2800-PG) coated at 5 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse pIgR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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pIgR in Mouse Liver. pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: pIgR

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

References

de Groot, N. et al. (1999) Transgenic Res. 8:125.
Piskurich, J. et al. (1995) J. Immunol. 154:1735.
Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
Asano, M. et al. (2004) Immunology 112:583.
Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
Uren, T. et al. (2003) J. Immunol. 170:2531.

Long Name

Polymeric Immunoglobulin Receptor

Entrez Gene IDs

5284 (Human); 18703 (Mouse)

Alternate Names

FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor

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Citations for Mouse pIgR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Lung immunoglobulin A immunity dysregulation in cystic fibrosis
Authors: AM Collin, M Lecocq, S Noel, B Detry, FM Carlier, F Aboubakar, C Bouzin, T Leal, M Vermeersch, V De Rose, L Regard, C Martin, PR Burgel, D Hoton, S Verleden, A Froidure, C Pilette, S Gohy
EBioMedicine, 2020-09-11;60(0):102974.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein
Authors: B Zeng, D Wang, H Wang, T Chen, J Luo, Q Xi, J Sun, Y Zhang
Nutrients, 2020-09-08;12(9):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot
Single-cell mapping of the thymic stroma identifies IL-25-producing tuft epithelial cells
Authors: C Bornstein, S Nevo, A Giladi, N Kadouri, M Pouzolles, F Gerbe, E David, A Machado, A Chuprin, B Tóth, O Goldberg, S Itzkovitz, N Taylor, P Jay, VS Zimmermann, J Abramson, I Amit
Nature, 2018-07-18;559(7715):622-626.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Loss of CD14 leads to disturbed epithelial-B cell crosstalk and impairment of the intestinal barrier after E. coli Nissle monoassociation
Authors: M Basic, M Buettner, LM Keubler, A Smoczek, I Bruesch, S Buchheiste, A Bleich
Sci Rep, 2018-01-15;8(1):719.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces
Authors: KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler
JCI Insight, 2017-08-17;2(16):.
Species: Mouse
Sample Types: BALF, Tissue Homogenates
Applications: Western Blot
Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA
Authors: K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai
Sci Rep, 2017-04-03;7(0):45843.
Species: Plant
Sample Types: Crude Leaf Extracts, Whole Tissue
Applications: ELISA Development (Capture), IHC
Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease.
Authors: Olsan E, Matsushita T, Rezaei M, Weimbs T
J Biol Chem, 2015-04-28;290(25):15679-86.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
Authors: Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T
Nature, 2015-02-16;521(7550):90-3.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation.
Authors: Hegab, Ahmed E, Ha, Vi Luan, Bisht, Bharti, Darmawan, Daphne O, Ooi, Aik T, Zhang, Kelvin X, Paul, Manash K, Kim, Yeon Sun, Gilbert, Jennifer, Attiga, Yasser S, Alva-Ornelas, Jackelyn, Nickerson, Derek W, Gomperts, Brigitte
Stem Cells Dev, 2014-01-02;23(6):664-75.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
Authors: Moon C, VanDussen K, Miyoshi H, Stappenbeck T
Mucosal Immunol, 2013-11-13;7(4):818-28.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomark Insights, 2012-07-10;7(0):87-104.
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
Authors: Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C
Mucosal Immunol, 2012-04-11;5(5):501-12.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
Authors: Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA
Proteomics, 2010-02-01;10(3):520-31.
Species: Mouse
Sample Types: BALF
Applications: Western Blot

Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
Authors: Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN
Vaccine, 2010-01-08;28(11):2311-7.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development

The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
Authors: Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA
Vaccine, 2010-01-07;28(10):2249-58.
Species: Mouse
Sample Types: Mucosal Lavage Fluid
Applications: ELISA Development