Mouse pIgR Antibody

(11 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse pIgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human pIgR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse pIgR
    Lys19-Lys645
    Accession # O70570
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.5-2.5 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of mouse IgM to immobilized Recombinant Mouse pIgR (Catalog # 2800-PG) coated at 5 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse pIgR by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for pIgR at approximately 85 to 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
pIgR in Mouse Liver. pIgR was detected in immersion fixed frozen sections of mouse liver using Goat Anti-Mouse pIgR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2800) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit  (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cytoplasm and plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: pIgR

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

  • References:
    1. de Groot, N. et al. (1999) Transgenic Res. 8:125.
    2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
    3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
    4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
    5. Asano, M. et al. (2004) Immunology 112:583.
    6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
    7. Uren, T. et al. (2003) J. Immunol. 170:2531.
  • Long Name:
    Polymeric Immunoglobulin Receptor
  • Entrez Gene IDs:
    5284 (Human); 18703 (Mouse)
  • Alternate Names:
    FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces
    Authors: KM Azzam, JH Madenspach, DW Cain, L Lai, KM Gowdy, P Rai, K Janardhan, N Clayton, W Cunningham, H Jensen, PS Patel, JF Kearney, GA Taylor, MB Fessler
    JCI Insight, 2017;2(16):.
    Species: Mouse
    Sample Type: BALF
  2. Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA
    Authors: K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai
    Sci Rep, 2017;7(0):45843.
    Species: Plant
    Sample Type: Crude Leaf Extracts
    Application: ELISA Capture
  3. Vertically transmitted faecal IgA levels determine extra-chromosomal phenotypic variation.
    Authors: Moon C, Baldridge M, Wallace M, Burnham C, Virgin H, Stappenbeck T
    Nature, 2015;521(7550):90-3.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Exploitation of the Polymeric Immunoglobulin Receptor for Antibody Targeting to Renal Cyst Lumens in Polycystic Kidney Disease.
    Authors: Olsan E, Matsushita T, Rezaei M, Weimbs T
    J Biol Chem, 2015;290(25):15679-86.
  5. Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis.
    Authors: Moon C, VanDussen K, Miyoshi H, Stappenbeck T
    Mucosal Immunol, 2014;7(4):818-28.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Aldehyde dehydrogenase activity enriches for proximal airway basal stem cells and promotes their proliferation.
    Authors: Hegab, Ahmed E, Ha, Vi Luan, Bisht, Bharti, Darmawan, Daphne O, Ooi, Aik T, Zhang, Kelvin X, Paul, Manash K, Kim, Yeon Sun, Gilbert, Jennifer, Attiga, Yasser S, Alva-Ornelas, Jackelyn, Nickerson, Derek W, Gomperts, Brigitte
    Stem Cells Dev, 2014;23(6):664-75.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. Targeted deletion of MyD88 in intestinal epithelial cells results in compromised antibacterial immunity associated with downregulation of polymeric immunoglobulin receptor, mucin-2, and antibacterial peptides.
    Authors: Frantz A, Rogier E, Weber C, Shen L, Cohen D, Fenton L, Bruno M, Kaetzel C
    Mucosal Immunol, 2012;5(5):501-12.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Type: BALF
    Application: WB
  9. The member of the cyclic di-nucleotide family bis-(3', 5')-cyclic dimeric inosine monophosphate exerts potent activity as mucosal adjuvant.
    Authors: Libanova R, Ebensen T, Schulze K, Bruhn D, Norder M, Yevsa T, Morr M, Guzman CA
    Vaccine, 2010;28(10):2249-58.
    Species: Mouse
    Sample Type: Mucosal Lavage Fluid
    Application: ELISA Development
  10. Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens.
    Authors: Shim DH, Ko HJ, Volker G, Potter AA, Mutwiri G, Babiuk LA, Kweon MN
    Vaccine, 2010;28(11):2311-7.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  11. Adjuvant effects of ambient particulate matter monitored by proteomics of bronchoalveolar lavage fluid.
    Authors: Kang X, Li N, Wang M, Boontheung P, Sioutas C, Harkema JR, Bramble LA, Nel AE, Loo JA
    Proteomics, 2010;10(3):520-31.
    Species: Mouse
    Sample Type: BALF
    Application: WB
Expand to show all 11 Citations
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