Mouse PLET-1 Antibody

Catalog #: MAB6917 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6917
MAB6917-SP

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Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse PLET-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse PLET-1 in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 700738

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PLET-1
Ala27-Leu216
Accession # Q8VEN2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

8-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunohistochemistry

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PLET‑1 in Mouse Kidney. PLET-1 was detected in immersion fixed frozen sections of adult mouse kidney using Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to medulla collecting ducts. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLET-1

PLET-1 (placenta-expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI-anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET-1, which most likely includes amino acids (aa) 28-216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the C‑terminus. PLET-1 is a marker of early thymic progenitor cells and pancreatic duct epithelium.

Long Name

Placenta-expressed Transcript 1

Entrez Gene IDs

76509 (Mouse)

Alternate Names

AgK114; C11orf34; chromosome 11 open reading frame 34; placenta expressed transcript 1; PLET1; PLET-1