Mouse PLET-1 Antibody

Catalog # Availability Size / Price Qty
Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry.
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Product Details
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Mouse PLET-1 Antibody Summary

Species Reactivity
Detects mouse PLET-1 in direct ELISAs.
Monoclonal Rat IgG1 Clone # 700738
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse PLET-1
Accession # Q8VEN2
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
2.5 µg/106 cells
See below
8-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PLET-1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of PLET-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Immunohistochemistry PLET-1 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

PLET‑1 in Mouse Kidney. PLET-1 was detected in immersion fixed frozen sections of adult mouse kidney using Rat Anti-Mouse PLET-1 Monoclonal Antibody (Catalog # MAB6917) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to medulla collecting ducts. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLET-1

PLET-1 (placenta-expressed transcript 1), also known as antigen mAgK114, is a 23 kDa membrane protein that is probably GPI-anchored. It is recognized by the antibodies MTS20 and MTS24. Mature mouse PLET-1, which most likely includes amino acids (aa) 28-216 of 237 aa, shares less than 60% aa sequence identity with the most closely related rat or human protein. A potential 194 aa mouse splice variant diverges after aa 149 but still contains a hydrophobic sequence at the C‑terminus. PLET-1 is a marker of early thymic progenitor cells and pancreatic duct epithelium.

Long Name
Placenta-expressed Transcript 1
Entrez Gene IDs
76509 (Mouse)
Alternate Names
AgK114; C11orf34; chromosome 11 open reading frame 34; placenta expressed transcript 1; PLET1; PLET-1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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