Detects mouse Plexin A1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 10% cross‑reactivity with recombinant mouse (rm) Plexin A3 is observed and no cross-reactivity with rmPlexin A2, recombinant human (rh) Plexin B1, rhPlexin C1, or rhPlexin D1 is observed.
Monoclonal Rat IgG2A Clone # 408305
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Plexin A1 Leu49-Gly165 Accession # P70206
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Plexin A1 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse Plexin A1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4309G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Plexin A1
Plexin-A1 is an 1894 amino acid, 200 kDa type I transmembrane protein that is a member of the Plexin family of semaphorin signal transducers. Plexin-A1 binds the transmembrane Sema6D directly and binds the Class 3 secreted semaphorins indirectly via neuropilin-1 and 2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. In the region used as an immunogen, mouse and human Plexin A1 share 97% amino acid identity.
Have you used Mouse Plexin A1 Alexa Fluor® 488-conjugated Antibody?
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