Mouse Podocalyxin Antibody

Catalog # Availability Size / Price Qty
AF1556
AF1556-SP

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Detection of Mouse Podocalyxin by Western Blot.
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Product Details
Citations (86)
FAQs
Supplemental Products
Reviews (5)

Mouse Podocalyxin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
D3 mouse embryonic stem cell line; Neuro-2A mouse neuroblastoma cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Podocalyxin antibody by Western Blot. View Larger

Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Podocalyxin antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Defects in TRAF3IP1 mutants are mediated by MAP4.(a) Lateral views of WT zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in WT embryos injected with control or map4 morpholino. (b) Lateral views of elipsa zebrafish embryos injected with map4 morpholino at 48 hpf and phenotype distribution in elipsa mutant embryos injected with control or map4 morpholino (data shown as combined result of n=3 independent experiments). Scale bars, 1 mm. (c) Relative expression of Map4 normalized to that of Hprt was analysed by qPCR in control and Traf3ip1-KD mIMCD3 cells stably expressing GFP or GFP-IFT54 mutants and Map4 shRNA. (d) Control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions were fixed in MeOH and stained for acetylated alpha -tubulin (red) and gamma -tubulin (light blue). Scale bar, 10 μm. (e) Six hours after Ca2+ switch, mIMCD3 cells grown until confluence on filters were fixed with 4% PFA and stained for the apical marker Gp135 (red). Scale bar, 10 μm. (f) Percentage of normal spheroids of control and Traf3ip1-KD/ Map4-KD mIMCD3 cells expressing either GFP or IFT54-GFP fusions grown on Matrigel for 5 days (mean ± s.d., n≥100 spheroids from 3 independent experiments, ***P≤0,0001, *P<0.012, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Gdf2 deletion decreases tumor perfusion and maturation in the E0771 mammary cancer model. E0771 cells were injected in the 4th mammary gland of WT and Gdf2−/− mice and tumor vascularization was analyzed 9 days after tumor detection. a Representative images of the tumors stained for podocalyxin (red), lectin (green) and cell nuclei (blue, Hoechst). Scale bar 50 μm. b Vascular density quantified by podocalyxin positive area (% of tumor area) and (c) assessment of vessel diameter using Ferret’s theorem (WT n = 7, Gdf2−/−n = 13, 1 representative experiment out of 2). d Quantification of vessel perfusion by lectin staining (% area of lectin/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). e Representative images of the tumors stained for podocalyxin (red), alpha -smooth muscle actin ( alpha -SMA) (green) and cell nuclei (blue, Hoechst). Scale bar 100 μm. f alpha -SMA staining quantification (% area of alpha -SMA/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). b, c, d, f Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. *p ≤ 0.05 and **p ≤ 0.01 significantly different Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30165893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Confocal ExM images of mouse kidney labeled with antibodies or fluorescent proteins. (a–c) Single focal plane of glomerulus immunostained for podocin (a), agrin (b), podocalyxin (Podxl c), and merge (d) of (a–c). (e–g) Confocal maximum intensity projections of glomerulus immunostained for synaptopodin (Synpo, e), acetylated tubulin (acTub, f), podocin (g) highlighting secondary FPs, primary FPs, and slit diaphragms/FP boundaries, respectively. (h) Merge of (e–h). (i–k) Confocal maximum intensity projections of glomerulus immunostained for collagen IV (Coll IV, i), podocalyxin (Podxl, j), and alpha smooth muscle actin ( alpha SMA, k) and highlighting Bowman’s capsule and the mesangium, podocytes, and arterioles and the mesangium, respectively. (l) Merge of (i–k). (m–p) Single focal plane of glomerulus showing native fluorescence from confetti mouse expressing YFP (m) and RFP (o) in separate podocyte cell bodies and FPs as well as GFP (n) in various podocyte nuclei. (p) Merge of (m–o). (q) Zoomed-in view of region highlighted in (p). (r) Further zoomed-in view (top) and cross-sectional profile (bottom) of boxed region highlighted in (q). All distances and scale bars are in pre-expansion units. Scale bars, 2 µm (a–h,q), 25 µm (i–l), 5 µm (m–p). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29991751), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Endothelial beta -catenin GOF does not affect the ECM of astrocytic endfeet and ECs within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of Lama2 and Aqp4 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Striatal BBB-vessel showing a polarized distribution of ColIV (green) but no Meca32 (white) in ECs (D). Coronal overview SFO, rectangular inset demarcates area for higher magnification in F (E); white dashed lines show Meca32+, red dashed lines show Meca32 vessels (F). Dashed lines outline SFO vessels; scale bars show 2 µm (A), 50 µm (B), 10 µm (C), 2.5 µm (D), 50 µm (E), 10 µm (F). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence TRAF3IP1 mutations lead to epithelialization and polarity defects.(a) mIMCD3 cells grown until confluence on filters were subjected to Ca2+-free medium to disrupt the tight junctions. Six hours after Ca2+ addition, cells were analysed by immunofluorescence using the apical marker Gp135 (red) and beta -catenin (light blue) to stain the cell junctions. Scale bar, 10 μm. (b) Following Ca2+ switch, tight junction re-formation was assessed by measurement of trans-epithelial resistance (TER) at different time points (mean ± s.e.m. of n=5 independent experiments, two-way ANOVA; NS: not-significant, ***P<0.001 at 6 h). (c) Height of mIMCD3 cells grown on filters measured as the distance from the base to the top of the cells (GFP staining, not shown; mean ± s.d. of n≥20, from 3 independent experiments, ***P<0.001, Bonferonni's multiple-comparison test). (d) Expression of the apical marker Gp135 was analysed by Western blot with alpha -tubulin as a loading control. (e) mIMCD3 cells grown in matrigel 3D matrix to form spheroids were stained for ZO1 (tight junctions, red) and analysed by confocal microscopy. Arrows indicate ZO-1 at the apical junctions, while arrow heads point to mislocalized ZO-1. Equatorial sections of representative spheres are shown for each cell line. Scale bars, 10 μm. (f) Percentage of abnormal spheroids (no/small lumen filled with cells) (mean ± s.d., n=80 spheroids from 2 independent experiments, ***P≤0,001, **P<0.002, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Bmp10 conditional deletion has no impact on tumor growth, angiogenesis and lung metastasis in the E0771 mammary cancer model. a Schematic representation of the experimental protocol for Bmp10 specific deletion and E0771 cells implantation. Tamoxifen was injected in all 3-week-old mice; 3 weeks later, E0771 cells were injected and tumor growth was analyzed for 3 weeks. b Plasmatic levels of BMP10 in control (CTL, n = 15) and Bmp10 conditional KO (Bmp10-cKO, n = 15) mice assessed by ELISA at the end of the experiment. c Tumor growth was assessed by caliper measurement every 2 to 3 days after tumor detection (CTL n = 7, Bmp10-cKO n = 8, 1 representative experiment out of 3). d Representative images of the tumors stained for podocalyxin (red), lectin (green) and cell nuclei (blue, Hoechst). Scale bar 50 μm. e Vascular density quantified by podocalyxin surface area (% of tumor area) and (f) Quantification of vessel perfusion by lectin staining (% area of lectin/podocalyxin) (CTL n = 7, Bmp10-cKO n = 8, 1 representative experiment out of 3). g Total area, (h) number and (i) mean size of lung metastases per mice bearing metastases (CTL n = 10, Bmp10-cKO n = 9, 2 experiments). c Data are the mean ± SEM. Statistical analysis: Two-way matched ANOVA. b, e, f, g, h, i Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. ****p ≤ 0.001 significantly different Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30165893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Endothelial beta -catenin GOF does not affect astrocytic endfoot polarization of alpha -dystroglycan ( alpha -Dag) and Kir4.1 within the subfornical organ (SFO).Striatal BBB-vessel showing a polarized distribution of alpha -Dag and Kir4.1 in AC endfeet. Lumen is stained by Podxl (asterisk) (A). Coronal overview of the subfornical organ (SFO) (B); rectangular inset demarcates area for higher magnification in (C). Dashed lines outline SFO vessels. Scale bar show 2 µm (A), 50 µm (B), 10 µm (C). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30932814), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse Podocalyxin Like by Western Blot View Larger

Detection of Mouse Podocalyxin Like by Western Blot TRAF3IP1 mutations lead to epithelialization and polarity defects.(a) mIMCD3 cells grown until confluence on filters were subjected to Ca2+-free medium to disrupt the tight junctions. Six hours after Ca2+ addition, cells were analysed by immunofluorescence using the apical marker Gp135 (red) and beta -catenin (light blue) to stain the cell junctions. Scale bar, 10 μm. (b) Following Ca2+ switch, tight junction re-formation was assessed by measurement of trans-epithelial resistance (TER) at different time points (mean ± s.e.m. of n=5 independent experiments, two-way ANOVA; NS: not-significant, ***P<0.001 at 6 h). (c) Height of mIMCD3 cells grown on filters measured as the distance from the base to the top of the cells (GFP staining, not shown; mean ± s.d. of n≥20, from 3 independent experiments, ***P<0.001, Bonferonni's multiple-comparison test). (d) Expression of the apical marker Gp135 was analysed by Western blot with alpha -tubulin as a loading control. (e) mIMCD3 cells grown in matrigel 3D matrix to form spheroids were stained for ZO1 (tight junctions, red) and analysed by confocal microscopy. Arrows indicate ZO-1 at the apical junctions, while arrow heads point to mislocalized ZO-1. Equatorial sections of representative spheres are shown for each cell line. Scale bars, 10 μm. (f) Percentage of abnormal spheroids (no/small lumen filled with cells) (mean ± s.d., n=80 spheroids from 2 independent experiments, ***P≤0,001, **P<0.002, Bonferonni's multiple-comparison test). Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/ncomms9666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Podocalyxin Like by Immunocytochemistry/Immunofluorescence Gdf2 deletion decreases tumor perfusion and maturation in the E0771 mammary cancer model. E0771 cells were injected in the 4th mammary gland of WT and Gdf2−/− mice and tumor vascularization was analyzed 9 days after tumor detection. a Representative images of the tumors stained for podocalyxin (red), lectin (green) and cell nuclei (blue, Hoechst). Scale bar 50 μm. b Vascular density quantified by podocalyxin positive area (% of tumor area) and (c) assessment of vessel diameter using Ferret’s theorem (WT n = 7, Gdf2−/−n = 13, 1 representative experiment out of 2). d Quantification of vessel perfusion by lectin staining (% area of lectin/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). e Representative images of the tumors stained for podocalyxin (red), alpha -smooth muscle actin ( alpha -SMA) (green) and cell nuclei (blue, Hoechst). Scale bar 100 μm. f alpha -SMA staining quantification (% area of alpha -SMA/podocalyxin) (WT n = 8, Gdf2−/− n = 7, 1 representative experiment out of 3). b, c, d, f Data are the median ± interquartile range. Statistical analysis: Mann-Whitney test. *p ≤ 0.05 and **p ≤ 0.01 significantly different Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30165893), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry View Larger

Detection of Podocalyxin in Neuro-2A cells by Flow Cytometry Neuro-2A cells were stained with Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556, filled histogram) or isotype control antibody (Catalog # 4-001-A, open histogram) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

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Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citations for Mouse Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

86 Citations: Showing 1 - 10
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  1. TIMP-2 and IGFBP7 in human kidney biopsies in renal disease
    Authors: Moritz Schanz, Martin Kimmel, Mark Dominik Alscher, Kerstin Amann, Christoph Daniel
    Clinical Kidney Journal
  2. Partial Mural Cell Ablation Disrupts Coronary Vasculature Integrity and Induces Systolic Dysfunction
    Authors: Cornuault, L;Hérion, FX;Bourguignon, C;Rouault, P;Foussard, N;Alzieu, P;Chapouly, C;Gadeau, AP;Couffinhal, T;Renault, MA;
    Journal of the American Heart Association
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Authors: Chen, X;Wolfe, DA;Bindu, DS;Zhang, M;Taskin, N;Goertsen, D;Shay, TF;Sullivan, EE;Huang, SF;Ravindra Kumar, S;Arokiaraj, CM;Plattner, VM;Campos, LJ;Mich, JK;Monet, D;Ngo, V;Ding, X;Omstead, V;Weed, N;Bishaw, Y;Gore, BB;Lein, ES;Akrami, A;Miller, C;Levi, BP;Keller, A;Ting, JT;Fox, AS;Eroglu, C;Gradinaru, V;
    Nature communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Scalable projected Light Sheet Microscopy for high-resolution imaging of large samples
    Authors: Chen, Y;Gong, C;Chauhan, S;De La Cruz, ED;Datta, MS;Tomer, R;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Whole-mount immunofluorescence staining of blood and lymphatic vessels in murine small intestine
    Authors: Satu Paavonsalo, Yelin Subashi, Madeleine H. Lackman, Sinem Karaman
    STAR Protocols
  6. Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse
    Authors: Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong et al.
    Frontiers in Cell and Developmental Biology
  7. Altered hemodynamics and vascular reactivity in a mouse model with severe pericyte deficiency
    Authors: Stobart JL, Erlebach E, Gl�ck C et al.
    Journal of Cerebral Blood Flow & Metabolism
  8. Functional gene delivery to and across brain vasculature of systemic AAVs with endothelial-specific tropism in rodents and broad tropism in primates
    Authors: Xinhong Chen, Damien A. Wolfe, Dhanesh Sivadasan Sivadasan Bindu, Mengying Zhang, Naz Taskin, David Goertsen et al.
    bioRxiv
  9. Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
    Authors: IS Zaitoun, YS Song, HB Zaitoun, CM Sorenson, N Sheibani
    Cells, 2022-10-21;11(20):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts
    Authors: Peter Baluk, Donald M. McDonald
    Methods in Molecular Biology
  11. Severe cerebellar malformations in mutant mice demonstrate a role for PDGF-C/PDGFR alpha signalling in cerebellar development
    Authors: Sara Gillnäs, Radiosa Gallini, Liqun He, Christer Betsholtz, Johanna Andrae
    Biology Open
  12. Conserved meningeal lymphatic drainage circuits in mice and humans
    Authors: Laurent Jacob, Jose de Brito Neto, Stephanie Lenck, Celine Corcy, Farhat Benbelkacem, Luiz Henrique Geraldo et al.
    Journal of Experimental Medicine
  13. Interplay of vascular endothelial growth factor receptors in organ-specific vessel maintenance
    Authors: Sinem Karaman, Satu Paavonsalo, Krista Heinolainen, Madeleine H. Lackman, Amanda Ranta, Karthik A. Hemanthakumar et al.
    Journal of Experimental Medicine
  14. Role of miRNA-671-5p in Mediating Wnt/ beta -Catenin-Triggered Podocyte Injury
    Authors: Chunhong Wang, Jiafeng Liu, Xiaoyao Zhang, Qiyan Chen, Xiaoyan Bai, Xue Hong et al.
    Frontiers in Pharmacology
  15. High-resolution fluorescence-guided transcranial ultrasound mapping in the live mouse brain
    Authors: H Estrada, J Robin, A Özbek, Z Chen, A Marowsky, Q Zhou, D Beck, B le Roy, M Arand, S Shoham, D Razansky
    Science Advances, 2021-12-08;7(50):eabi5464.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Imbalanced Activation of Wnt-/ beta -Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation
    Authors: Philipp-Sebastian Koch, Kajetan Sandorski, Joschka Heil, Christian D. Schmid, Sina W. Kürschner, Johannes Hoffmann et al.
    Frontiers in Physiology
  17. Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures
    Authors: K Gkatzis, P Panza, S Peruzzo, DY Stainier
    Elife, 2021-09-29;10(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  18. Characterization of the blood–brain barrier in genetically diverse laboratory mouse strains
    Authors: Johanna Schaffenrath, Sheng-Fu Huang, Tania Wyss, Mauro Delorenzi, Annika Keller
    Fluids and Barriers of the CNS
  19. A mouse model of prenatal exposure to Interleukin-6 to study the developmental origin of health and disease
    Authors: T Srivastava, T Joshi, DP Heruth, MH Rezaiekhal, RE Garola, J Zhou, VC Boinpelly, MF Ali, US Alon, M Sharma, GB Vanden Heu, P Mahajan, L Priya, Y Jiang, ET McCarthy, VJ Savin, R Sharma, M Sharma
    Scientific Reports, 2021-06-24;11(1):13260.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Mast Cells Are the Trigger of Small Vessel Disease and Diastolic Dysfunction in Diabetic Obese Mice
    Authors: Sarah Guimbal, Lauriane Cornuault, Paul Rouault, Pierre-Louis Hollier, Candice Chapouly, Marie-Lise Bats et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  21. Talin-dependent integrin activation is required for endothelial proliferation and postnatal angiogenesis
    Authors: Fadi E. Pulous, Jamie C. Carnevale, Zaki Al-Yafeai, Brenna H. Pearson, Jamie A. G. Hamilton, Curtis J. Henry et al.
    Angiogenesis
  22. Hepatocyte growth factor-regulated tyrosine kinase substrate is essential for endothelial cell polarity and cerebrovascular stability
    Authors: Zhenyang Yu, Jian Zeng, Jun Wang, Yaxiong Cui, Xiaopeng Song, Yizhe Zhang et al.
    Cardiovascular Research
  23. Role of IRE1 alpha in podocyte proteostasis and mitochondrial health
    Authors: José R. Navarro-Betancourt, Joan Papillon, Julie Guillemette, Takao Iwawaki, Chen-Fang Chung, Andrey V. Cybulsky
    Cell Death Discovery
  24. Single nuclei RNA-seq of mouse placental labyrinth development
    Authors: Bryan Marsh, Robert Blelloch
    eLife
  25. Mapping endothelial-cell diversity in cerebral cavernous malformations at single-cell resolution
    Authors: Fabrizio Orsenigo, Lei Liu Conze, Suvi Jauhiainen, Monica Corada, Francesca Lazzaroni, Matteo Malinverno et al.
    eLife
  26. Cytotoxic T-cells mediate exercise-induced reductions in tumor growth
    Authors: Helene Rundqvist, Pedro Veliça, Laura Barbieri, Paulo A Gameiro, David Bargiela, Milos Gojkovic et al.
    eLife
  27. Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function
    Authors: Amanda J. Eakin, Tamara Mc Erlain, Aileen Burke, Amy Eaton, Nuala Tipping, Gloria Allocca et al.
    Frontiers in Cell and Developmental Biology
  28. Feature-rich covalent stains for super-resolution and cleared tissue fluorescence microscopy
    Authors: C Mao, MY Lee, JR Jhan, AR Halpern, MA Woodworth, AK Glaser, TJ Chozinski, L Shin, JW Pippin, SJ Shankland, JTC Liu, JC Vaughan
    Sci Adv, 2020-05-27;6(22):eaba4542.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome
    Authors: M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama
    Nat Commun, 2020-03-12;11(1):1343.
    Species: Mouse
    Sample Types: Cells
    Applications: ICC
  30. Genetic Ablation of Calcium-independent Phospholipase A2 gamma Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice
    Authors: Hanan Elimam, Joan Papillon, Julie Guillemette, José R. Navarro-Betancourt, Andrey V. Cybulsky
    Scientific Reports
  31. The coronary artery disease risk-associated Plpp3 gene and its product lipid phosphate phosphatase 3 regulate experimental atherosclerosis
    Authors: Paul A. Mueller, Liping Yang, Margo Ubele, Guogen Mao, Jason Brandon, Julia Vandra et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  32. Retinoic acid receptor alpha as a novel contributor to adrenal cortex structure and function through interactions with Wnt and Vegfa signalling
    Authors: RM El Zein, AH Soria, JF Golib Dzib, AJ Rickard, FL Fernandes-, B Samson-Cou, I Giscos-Dou, A Rocha, M Poglitsch, CE Gomez-Sanc, L Amar, NB Ghyselinck, A Benecke, MC Zennaro, S Boulkroun
    Sci Rep, 2019-10-11;9(1):14677.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. Vascular regression precedes motor neuron loss in the FUS (1-359) ALS mouse model
    Authors: Martin Crivello, Marion C. Hogg, Elisabeth Jirström, Luise Halang, Ina Woods, Megan Rayner et al.
    Disease Models & Mechanisms
  34. Acute and chronic hypoxia differentially predispose lungs for metastases
    Authors: M Reiterer, R Colaço, P Emrouzneja, A Jensen, H Rundqvist, RS Johnson, C Branco
    Sci Rep, 2019-07-15;9(1):10246.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. Evaporative cooling provides a major metabolic energy sink
    Authors: I Kasza, D Adler, DW Nelson, CL Eric Yen, S Dumas, JM Ntambi, OA MacDougald, D Hernando, WP Porter, FA Best, CM Alexander
    Mol Metab, 2019-07-01;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  36. Loss of the transcription factor RBPJ induces disease-promoting properties in brain pericytes
    Authors: R Diéguez-Hu, K Kato, BD Giaimo, M Nieminen-K, H Arf, F Ferrante, M Bartkuhn, T Zimmermann, MG Bixel, HM Eilken, S Adams, T Borggrefe, P Vajkoczy, RH Adams
    Nat Commun, 2019-06-27;10(1):2817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. Endothelial cell clonal expansion in the development of cerebral cavernous malformations
    Authors: M Malinverno, C Maderna, A Abu Taha, M Corada, F Orsenigo, M Valentino, F Pisati, C Fusco, P Graziano, M Giannotta, QC Yu, YA Zeng, MG Lampugnani, PU Magnusson, E Dejana
    Nat Commun, 2019-06-24;10(1):2761.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  38. Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis
    Authors: G Genet, K Boyé, T Mathivet, R Ola, F Zhang, A Dubrac, J Li, N Genet, L Henrique G, L Benedetti, S Künzel, L Pibouin-Fr, JL Thomas, A Eichmann
    Nat Commun, 2019-05-28;10(1):2350.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  39. Foxc2 is essential for podocyte function
    Authors: D Nilsson, M Heglind, Z Arani, S Enerbäck
    Physiol Rep, 2019-05-01;7(9):e14083.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  40. Wnt/ beta -catenin links oxidative stress to podocyte injury and proteinuria
    Authors: Lili Zhou, Xiaowen Chen, Meizhi Lu, Qinyu Wu, Qian Yuan, Chengxiao Hu et al.
    Kidney International
  41. Maresin 1 attenuates neuroinflammation in a mouse model of perioperative neurocognitive disorders
    Authors: T. Yang, G. Xu, P.T. Newton, A.S. Chagin, S. Mkrtchian, M. Carlström et al.
    British Journal of Anaesthesia
  42. aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability
    Authors: M Riddell, A Nakayama, T Hikita, F Mirzapours, T Kawamura, A Pasha, M Li, M Masuzawa, M Looso, T Steinbache, K Ebnet, M Potente, T Hirose, S Ohno, I Fleming, S Gattenlöhn, PP Aung, T Phung, O Yamasaki, T Yanagi, H Umemura, M Nakayama
    Nat Commun, 2018-12-17;9(1):5357.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  43. APOL1 risk allele RNA contributes to renal toxicity by activating protein kinase R
    Authors: Koji Okamoto, Jason W. Rausch, Hidefumi Wakashin, Yulong Fu, Joon-Yong Chung, Patrick D. Dummer et al.
    Communications Biology
  44. Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele
    Authors: M Takahashi, M Tamura, S Sato, K Kawakami
    Dis Model Mech, 2018-10-25;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  45. PAR ‐3 controls endothelial planar polarity and vascular inflammation under laminar flow
    Authors: Takao Hikita, Fatemeh Mirzapourshafiyi, Pedro Barbacena, Meghan Riddell, Ayesha Pasha, Mengnan Li et al.
    EMBO reports
  46. BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer
    Authors: M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly
    J. Exp. Clin. Cancer Res., 2018-08-30;37(1):209.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  47. Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy
    Authors: TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan
    Sci Rep, 2018-07-10;8(1):10396.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Ste20-like kinase, SLK, a novel mediator of podocyte integrity
    Authors: Andrey V. Cybulsky, Joan Papillon, Julie Guillemette, Natalya Belkina, Genaro Patino-Lopez, Elena Torban
    American Journal of Physiology-Renal Physiology
  49. EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF
    Authors: E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi
    EMBO Rep., 2018-04-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. Extracellular retention of PDGF-B directs vascular remodeling in mouse hypoxia-induced pulmonary hypertension
    Authors: Philip Tannenberg, Ya-Ting Chang, Lars Muhl, Bàrbara Laviña, Hanna Gladh, Guillem Genové et al.
    American Journal of Physiology-Lung Cellular and Molecular Physiology
  51. Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice
    Authors: R Hatano, A Takeda, Y Abe, K Kawaguchi, I Kazama, M Matsubara, S Asano
    Sci Rep, 2018-03-14;8(1):4512.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  52. Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury
    Authors: DO Dias, H Kim, D Holl, B Werne Soln, J Lundeberg, M Carlén, C Göritz, J Frisén
    Cell, 2018-03-01;173(1):153-165.e22.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  53. Pleiotropic activity of systemically delivered angiogenin in the SOD1G93Amouse model
    Authors: M Crivello, SL O'Riordan, I Woods, S Cannon, L Halang, KS Coughlan, MC Hogg, SA Lewandowsk, JHM Prehn
    Neuropharmacology, 2018-02-25;133(0):503-511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  54. Neurog3-dependent pancreas dysgenesis causes ectopic pancreas in Hes1 mutant mice
    Authors: Mette C. Jørgensen, Kristian H. de Lichtenberg, Caitlin A. Collin, Rasmus Klinck, Jeppe H. Ekberg, Maja S. Engelstoft et al.
    Development
  55. Development and plasticity of meningeal lymphatic vessels
    Authors: Salli Antila, Sinem Karaman, Harri Nurmi, Mikko Airavaara, Merja H. Voutilainen, Thomas Mathivet et al.
    Journal of Experimental Medicine
  56. Loss of Vascular CD34 Results in Increased Sensitivity to Lung Injury
    Authors: Bernard C. Lo, Matthew J. Gold, Sebastian Scheer, Michael R. Hughes, Jessica Cait, Erin Debruin et al.
    American Journal of Respiratory Cell and Molecular Biology
  57. Genetic and pharmacological inhibition of microRNA-92a maintains podocyte cell cycle quiescence and limits crescentic glomerulonephritis
    Authors: Carole Henique, Guillaume Bollée, Xavier Loyer, Florian Grahammer, Neeraj Dhaun, Marine Camus et al.
    Nature Communications
  58. HS3ST1 genotype regulates antithrombin's inflammomodulatory tone and associates with atherosclerosis
    Authors: Nicole C. Smits, Takashi Kobayashi, Pratyaksh K. Srivastava, Sladjana Skopelja, Julianne A. Ivy, Dustin J. Elwood et al.
    Matrix Biology
  59. Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity
    Authors: Yixin Wang, Yi Jin, Maarja Andaloussi Mäe, Yang Zhang, Henrik Ortsäter, Christer Betsholtz et al.
    Development
  60. Loss of mucin-type O-glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney
    Authors: K Song, J Fu, J Song, BH Herzog, K Bergstrom, Y Kondo, JM McDaniel, S McGee, R Silasi-Man, F Lupu, H Chen, H Bagavant, L Xia
    J. Biol. Chem., 2017-08-25;292(40):16491-16497.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  61. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017-07-07;7(1):4916.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  62. Deletion of Inositol-Requiring Enzyme-1? in Podocytes Disrupts Glomerular Capillary Integrity and Autophagy
    Authors: DR Kaufman, J Papillon, L Larose, T Iwawaki, AV Cybulsky
    Mol. Biol. Cell, 2017-04-20;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  63. Compound genetically engineered mouse models of cancer reveal dual targeting of ALK1 and endoglin as a synergistic opportunity to impinge on angiogenic TGF-beta signaling
    Authors: Nikolas M. Eleftheriou, Jonas Sjölund, Matteo Bocci, Eliane Cortez, Se-Jin Lee, Sara I. Cunha et al.
    Oncotarget
  64. Vascular development in the vertebrate pancreas
    Authors: D. Berfin Azizoglu, Diana C. Chong, Alethia Villasenor, Judith Magenheim, David M. Barry, Simon Lee et al.
    Developmental Biology
  65. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016-08-05;6(0):30739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  66. Loss of endothelial barrier integrity in mice with conditional ablation of podocalyxin (Podxl) in endothelial cells
    Authors: Angélica Horrillo, Gracia Porras, Matilde S. Ayuso, Consuelo González-Manchón
    European Journal of Cell Biology
  67. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016-07-29;7(0):12329.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  68. CD34 Promotes Pathological Epi-Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy
    Authors: Martin J. Siemerink, Michael R. Hughes, Marchien G. Dallinga, Tomek Gora, Jessica Cait, Ilse M. C. Vogels et al.
    PLOS ONE
  69. Anti-metastatic action of FAK inhibitor OXA-11 in combination with VEGFR-2 signaling blockade in pancreatic neuroendocrine tumors
    Authors: Ingrid Moen, Matthew Gebre, Vanesa Alonso-Camino, Debbie Chen, David Epstein, Donald M. McDonald
    Clinical & Experimental Metastasis
  70. tPA Deficiency in Mice Leads to Rearrangement in the Cerebrovascular Tree and Cerebroventricular Malformations
    Authors: Christina Stefanitsch, Anna-Lisa E. Lawrence, Anna Olverling, Ingrid Nilsson, Linda Fredriksson
    Frontiers in Cellular Neuroscience
  71. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2015-11-19;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  72. Wt1 and beta -catenin cooperatively regulate diaphragm development in the mouse
    Authors: Nicole D. Paris, Garry L. Coles, Kate G. Ackerman
    Developmental Biology
  73. Identification of a neurovascular signaling pathway regulating seizures in mice
    Authors: Linda Fredriksson, Tamara K. Stevenson, Enming J. Su, Margaret Ragsdale, Shannon Moore, Stefan Craciun et al.
    Annals of Clinical and Translational Neurology
  74. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
    Authors: Cunha S, Bocci M, Lovrot J, Eleftheriou N, Roswall P, Cordero E, Lindstrom L, Bartoschek M, Haller B, Pearsall R, Mulivor A, Kumar R, Larsson C, Bergh J, Pietras K
    Cancer Res, 2015-06-15;75(12):2445-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC-Fr
  75. Mutual Antagonism of Wilms’ Tumor 1 and beta -Catenin Dictates Podocyte Health and Disease
    Authors: Lili Zhou, Yingjian Li, Weichun He, Dong Zhou, Roderick J. Tan, Jing Nie et al.
    Journal of the American Society of Nephrology
  76. Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A.
    Authors: Al-Momany A, Li L, Alexander R, Ballermann B
    J Cell Sci, 2014-10-24;127(24):5164-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  77. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
    Authors: Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T
    Am J Pathol, 2014-06-18;184(8):2211-24.
    Species: Mouse, Rat
    Sample Types: Whole Tissue
    Applications: ICC
  78. Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
    Authors: Lenoir O, Milon M, Virsolvy A, Henique C, Schmitt A, Masse J, Kotelevtsev Y, Yanagisawa M, Webb D, Richard S, Tharaux P
    J Am Soc Nephrol, 2014-04-10;25(5):1050-62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  79. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014-03-12;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  80. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2013-12-18;141(2):448-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  81. Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota.
    Authors: Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M
    Am J Pathol, 2013-10-03;183(6):1945-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  82. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
    Authors: Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, Franco M, Paez-Ribes M, Cordiner R, Fuxe J, Johansson B, Goumans M, Casanovas O, ten Dijke P, Arthur H, Pietras K
    J Exp Med, 2013-02-11;210(3):563-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  83. Integrin Beta 1 Suppresses Multilayering of a Simple Epithelium
    Authors: Jichao Chen, Mark A. Krasnow
    PLoS ONE
  84. Oral monosaccharide therapies to reverse renal and muscle hyposialylation in a mouse model of GNE myopathy
    Authors: Terren K. Niethamer, Tal Yardeni, Petcharat Leoyklang, Carla Ciccone, Adrian Astiz-Martinez, Katherine Jacobs et al.
    Molecular Genetics and Metabolism
  85. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010-07-21;78(9):868-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  86. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Mouse Podocalyxin Antibody
By Troy Trevino on 03/22/2023
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Brain (cerebellum) tissue Species: Mouse

Mouse Podocalyxin Antibody
By Ali Almousawi on 03/08/2023
Application: IHC Sample Tested: Brain (vasculature) Species: Mouse

Podocalyxin detection in formalin fixed paraffin embedded sections of mouse brain using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at a dilution of 1:500 overnight at 4 °C. DAPI and Hoechst 33342 were used together for nuclear stain. The podocalyxin staining denoted micro vessels in brain sections for endothelial cell barrier disruption analysis by probing for serum protein deposition proximal to brain microvasculature. This image was taken on a Zeiss LSM 710 Confocal Microscope (META) with Zeiss Plan Apo 63x/1.40 oil


Mouse Podocalyxin Antibody
By Anonymous on 05/04/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult brain Species: Mouse

Dilution 1:200


Mouse Podocalyxin Antibody
By Anonymous on 02/20/2017
Application: IHC Sample Tested: Adult heart Species: Mouse

10um think hearts sections. Fixed with 4% PFA at RT for 10mins, permeabilized using 0.3% triton for 30mins at RT followed by 1% BSA block. Incubated with Goat Podocalyxin (1/100 concentration) at 4’C – O/N. Washed using 1xPBS, Detected using Donkey anti-goat alexa 568 (1/200 concentration). Washed and mounted using prolong antifade reagent with DAPI


Mouse Podocalyxin Antibody
By Stephen Wilson on 04/22/2016
Sample Tested: Tracheal whole mount Species: Mouse

Immunofluorescence staining of whole mount tracheal blood vessels. 1:200 dilution