Mouse Podocalyxin Antibody

(13 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
    Ser21-Arg402
    Accession # Q9R0M4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    D3 mouse embryonic stem cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  • References:
    1. Li, J. et al. (2001) DNA Seq. 12(5):407.
    2. Hara, T. et al. (1999) Immunity 11(5):567.
  • Entrez Gene IDs:
    5420 (Human); 27205 (Mouse)
  • Alternate Names:
    GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017;7(1):4916.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016;7(0):12329.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  3. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016;6(0):30739.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
    Authors: Cunha S, Bocci M, Lovrot J, Eleftheriou N, Roswall P, Cordero E, Lindstrom L, Bartoschek M, Haller B, Pearsall R, Mulivor A, Kumar R, Larsson C, Bergh J, Pietras K
    Cancer Res, 2015;75(12):2445-56.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Frozen
  5. Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A.
    Authors: Al-Momany A, Li L, Alexander R, Ballermann B
    J Cell Sci, 2014;127(24):5164-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
    Authors: Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T
    Am J Pathol, 2014;184(8):2211-24.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: ICC Fluorescence
  7. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2014;141(2):448-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  8. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
    Authors: Lenoir O, Milon M, Virsolvy A, Henique C, Schmitt A, Masse J, Kotelevtsev Y, Yanagisawa M, Webb D, Richard S, Tharaux P
    J Am Soc Nephrol, 2014;25(5):1050-62.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota.
    Authors: Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M
    Am J Pathol, 2013;183(6):1945-59.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  11. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
    Authors: Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, Franco M, Paez-Ribes M, Cordiner R, Fuxe J, Johansson B, Goumans M, Casanovas O, ten Dijke P, Arthur H, Pietras K
    J Exp Med, 2013;210(3):563-79.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010;78(9):868-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005;16(7):1993-2002.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 13 Citations
Cell and Tissue Staining Kits
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 4.5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Immunofluorescence staining of whole mount tracheal blood vessels.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse Podocalyxin Antibody AF1556
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Very Good
 IHC Mouse Anonymous 02/20/2017
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Image Details
Immunohistochemistry Mouse Podocalyxin Antibody AF1556
Immunohistochemistry: Mouse Podocalyxin Antibody [AF1556]

Summary

ApplicationImmunohistochemistry
Sample TestedAdult heart
SpeciesMouse

Other Experimental Details

Other Experimental Details10um think hearts sections. Fixed with 4% PFA at RT for 10mins, permeabilized using 0.3% triton for 30mins at RT followed by 1% BSA block. Incubated with Goat Podocalyxin (1/100 concentration) at 4’C – O/N. Washed using 1xPBS, Detected using Donkey anti-goat alexa 568 (1/200 concentration). Washed and mounted using prolong antifade reagent with DAPI
Immunofluorescence staining of whole mount tracheal blood vessels Mouse Podocalyxin Antibody AF1556
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Excellent
 Immunofluorescence staining of whole mount tracheal blood vessels Mouse Anonymous 04/22/2016
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Image Details
Immunofluorescence staining of whole mount tracheal blood vessels Mouse Podocalyxin Antibody AF1556
Immunofluorescence staining of whole mount tracheal blood vessels: Mouse Podocalyxin Antibody [AF1556]

Summary

ApplicationImmunofluorescence staining of whole mount tracheal blood vessels
Sample TestedTracheal whole mount
SpeciesMouse

Other Experimental Details

Other Experimental Details1:200 dilution

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