Mouse Podocalyxin Antibody

Catalog # Availability Size / Price Qty
AF1556
AF1556-SP
Detection of Mouse Podocalyxin by Western Blot.
2 Images
Product Details
Citations (17)
FAQs
Supplemental Products
Reviews (2)

Mouse Podocalyxin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse Podocalyxin by Western Blot. View Larger

Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Podocalyxin in Mouse Thymus. View Larger

Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Citations for Mouse Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice
    Authors: R Hatano, A Takeda, Y Abe, K Kawaguchi, I Kazama, M Matsubara, S Asano
    Sci Rep, 2018;8(1):4512.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  2. Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy
    Authors: TJ Chozinski, C Mao, AR Halpern, JW Pippin, SJ Shankland, CE Alpers, B Najafian, JC Vaughan
    Sci Rep, 2018;8(1):10396.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Deletion of Inositol-Requiring Enzyme-1? in Podocytes Disrupts Glomerular Capillary Integrity and Autophagy
    Authors: DR Kaufman, J Papillon, L Larose, T Iwawaki, AV Cybulsky
    Mol. Biol. Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1
    Authors: SK Jha, K Rauniyar, T Karpanen, VM Leppänen, P Brouillard, M Vikkula, K Alitalo, M Jeltsch
    Sci Rep, 2017;7(1):4916.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice
    Sci Rep, 2016;6(0):30739.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  6. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2016;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  7. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance
    Nat Commun, 2016;7(0):12329.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC - Fresh
  8. Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
    Authors: Cunha S, Bocci M, Lovrot J, Eleftheriou N, Roswall P, Cordero E, Lindstrom L, Bartoschek M, Haller B, Pearsall R, Mulivor A, Kumar R, Larsson C, Bergh J, Pietras K
    Cancer Res, 2015;75(12):2445-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC Frozen
  9. Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
    Authors: Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T
    Am J Pathol, 2014;184(8):2211-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: ICC Fluorescence
  10. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  11. Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
    Authors: Lenoir O, Milon M, Virsolvy A, Henique C, Schmitt A, Masse J, Kotelevtsev Y, Yanagisawa M, Webb D, Richard S, Tharaux P
    J Am Soc Nephrol, 2014;25(5):1050-62.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  12. Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
    Authors: Fan J, Ponferrada V, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang R
    Development, 2014;141(2):448-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Not Specified
  13. Clustered PI(4,5)P(2) accumulation and ezrin phosphorylation in response to CLIC5A.
    Authors: Al-Momany A, Li L, Alexander R, Ballermann B
    J Cell Sci, 2014;127(24):5164-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
    Authors: Anderberg C, Cunha S, Zhai Z, Cortez E, Pardali E, Johnson J, Franco M, Paez-Ribes M, Cordiner R, Fuxe J, Johansson B, Goumans M, Casanovas O, ten Dijke P, Arthur H, Pietras K
    J Exp Med, 2013;210(3):563-79.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  15. Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) lambda/iota.
    Authors: Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber T, Schiffer M
    Am J Pathol, 2013;183(6):1945-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  16. Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
    Authors: Pierchala BA, Munoz MR, Tsui CC
    Kidney Int., 2010;78(9):868-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  17. Novel regulators of kidney development from the tips of the ureteric bud.
    Authors: Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
    J. Am. Soc. Nephrol., 2005;16(7):1993-2002.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Reviews for Mouse Podocalyxin Antibody

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Mouse Podocalyxin Antibody
By Anonymous on 02/20/2017
Application: IHC Sample Tested: Adult heart Species: Mouse

10um think hearts sections. Fixed with 4% PFA at RT for 10mins, permeabilized using 0.3% triton for 30mins at RT followed by 1% BSA block. Incubated with Goat Podocalyxin (1/100 concentration) at 4’C – O/N. Washed using 1xPBS, Detected using Donkey anti-goat alexa 568 (1/200 concentration). Washed and mounted using prolong antifade reagent with DAPI


Mouse Podocalyxin Antibody
By Anonymous on 04/22/2016
Application: Immunofluorescence staining of whole mount tracheal blood vessels Sample Tested: Tracheal whole mount Species: Mouse

1:200 dilution