Mouse PRDM14 Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PRDM14 in ELISA.
  • Source
    Monoclonal Rat IgG2B Clone # 856235
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse PRDM14
    Met1-Glu191
    Accession # E9Q3T6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
PRDM14 in D3 Mouse Embryonic Stem Cells. PRDM14 was detected in immersion fixed D3 mouse embryonic stem cells using Rat Anti-Mouse PRDM14 Monoclonal Antibody (Catalog # MAB8097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRDM14

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 243 – 360) and six zinc finger repeats (aa 390 - 558). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Within aa 1-191, mouse PRDM14 shares 46% aa sequence identity with human PRDM14.

  • Long Name:
    PR Domain Containing 14
  • Entrez Gene IDs:
    63978 (Human); 383491 (Mouse)
  • Alternate Names:
    MGC59730; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRDM14; PR-domain zinc finger protein 14
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency
    Stem Cell Reports, 2016;7(6):1072-1086.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. PRDM14 Drives OCT3/4 Recruitment via Active Demethylation in the Transition from Primed to Naive Pluripotency
    Stem Cell Reports, 2016;7(6):1072-1086.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
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Rat IgG2B Isotype Control

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Secondary Antibodies
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WB, Simple Western HAF005 7  
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Rat IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Rat IgG Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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