PRDM14 in D3 Mouse Embryonic Stem Cells. PRDM14 was detected in immersion fixed D3 mouse embryonic stem cells using Rat Anti-Mouse PRDM14 Monoclonal Antibody (Catalog # MAB8097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 243 – 360) and six zinc finger repeats (aa 390 - 558). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Within aa 1-191, mouse PRDM14 shares 46% aa sequence identity with human PRDM14.
PR Domain Containing 14
Entrez Gene IDs:
63978 (Human); 383491 (Mouse)
MGC59730; PFM11; PR domain containing 14; PR domain zinc finger protein 14; PR domain-containing protein 14; PRDM14; PR-domain zinc finger protein 14
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