Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB8634P-025
FAB8634P-100
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
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Mouse Pref-1/DLK1/FA1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Pref-1/DLK1/FA1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1168B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref‑1/DLK1/FA1 PE‑conjugated Monoclonal Antibody (Catalog # FAB8634P, filled histogram) or isotype control antibody (Catalog # IC105P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385).  It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively.

Long Name
Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1
Entrez Gene IDs
8788 (Human); 13386 (Mouse)
Alternate Names
delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

Product Datasheets

FAQs

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Isotype Controls

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