Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Simple Western

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1168B
View all Pref-1/DLK1/FA1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163

Specificity

Detects mouse Pref-1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse Pref‑1/DLK1/FA1 Antibody

Detection of Mouse Pref‑1/DLK1/FA1 antibody by Western Blot.

Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot.

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Mouse Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Pref-1/DLK1/FA1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Pref-1/DLK1/FA1 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Pref-1/DLK1/FA1 antibody in Mouse Embryonic Liver Tissue by Immunohistochemistry (IHC-Fr).

Pref‑1/DLK1/FA1 in Mouse Embryonic Liver Tissue.

Pref-1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic liver tissue (13 d.p.c.) using Rabbit Anti-Mouse Pref-1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Pref-1/DLK1/FA1 antibody by Simple WesternTM.

Detection of Mouse Pref‑1/DLK1/FA1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Pref‑1/DLK1/FA1 at approximately 63 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

Applications for Mouse Pref‑1/DLK1/FA1 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line

Immunohistochemistry

1-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryonic liver tissue (13 d.p.c.)

Simple Western

1 µg/mL
Sample: Mouse embryo tissue

Western Blot

0.1 µg/mL
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein.  It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385).  It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Alternate Names

DLK-1, DLK1, FA1, pG2, Pref1, ZOG

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Gene Symbol

DLK1

UniProt

Q09163

Additional Pref-1/DLK1/FA1 Products

Product Documents for Mouse Pref‑1/DLK1/FA1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Pref‑1/DLK1/FA1 Antibody

For research use only

Citations for Mouse Pref‑1/DLK1/FA1 Antibody

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Protocols

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