Detection of Mouse Pref‑1/DLK1/FA1 by Western Blot.
Western blot shows lysates of 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and mouse embryo tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Pref‑1/DLK1/FA1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.
3T3‑L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Pref‑1/DLK1/FA1 in Mouse Embryonic Liver Tissue.
Pref‑1/DLK1/FA1 was detected in immersion fixed frozen sections of mouse embryonic liver tissue (13 d.p.c.) using Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Mouse Pref‑1/DLK1/FA1 by Simple WesternTM.
Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Pref‑1/DLK1/FA1 at approximately 63 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Mouse Pref‑1/DLK1/FA1 Monoclonal Antibody (Catalog # MAB8634). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385). It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively
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