Mouse R-Spondin 1 Antibody

Catalog # Availability Size / Price Qty
AF3474
AF3474-SP
R‑Spondin 1 in Mouse Ovary.
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Product Details
Citations (7)
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Mouse R-Spondin 1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross‑reactivity with recombinant human R-Spondin 1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) R-Spondin 3 and rmR-Spondin 4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse R-Spondin 1 (R&D Systems, Catalog # 3474-RS)
Ser21-Gly209
Accession # Q9Z132
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry R-Spondin 1 antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr). View Larger

R‑Spondin 1 in Mouse Ovary. R-Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R-Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Western Blot Detection of Mouse R-Spondin 1 by Western Blot View Larger

Detection of Mouse R-Spondin 1 by Western Blot RSPO1 protein content and distribution pattern during male gonad development.Left panel: (Upper part) Western blot analysis of RSPO1 in 13.5, 16.5 and 18.5 dpc embryonic male gonads, and 13.5 dpc male germ cells. As RSPO1 western blot negative control 17.5 dpc intestine is also reported. Two different bands, corresponding to the immature (27 KDa band) and the glycosilated isoform (30 KDa band), were detected. (Lower part) Confocal microscopy analysis of RSPO1 distribution observed by whole mount immunofluorescence (FITC signal) in 15.5 dpc ovary (positive control) and in 13.5 dpc male germ cells. RSPO1 immuno-labeled germ cells were double stained with anti-VASA (germ cell marker) antibody. Right panel: Confocal microscopy analysis of RSPO1 distribution observed by whole mount immunofluorescence (FITC signal) in 11.5 (A), 12.5 (C and D), 13.5 (E and F), 16.5 (G and H), and 18.5 dpc (I and L) male UGRs at different magnifications. In B the corresponding bright field of 11.5 dpc male genital ridge is reported. ce: coelomic epithelium; gr: genital ridge; ms: mesonephros; tc: testicular cords; ts: testis, ov: ovary. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124213), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1 - 3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34 - 135) followed by a thrombospondin (TSP-1) motif (aa 147 - 207) and a region rich in basic residues (aa 211 - 263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether R‑Spondin 1 binds LRP-6 directly (10 - 12).

References
  1. Lowther, W. et al. (2005) J. Virol. 79:10093.
  2. Kim, K-A. et al. (2006) Cell Cycle 5:23.
  3. Nam, J-S. et al. (2007) Gene Expr. Patterns 7:306.
  4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
  5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
  6. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
  7. Parma, P. et al. (2006) Nat. Genet. 38:1304.
  8. Kim, K-A. et al. (2005) Science 309:1256.
  9. Zhao, J. et al. (2007) Gastroenterology 132:1331.
  10. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  11. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
  12. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse); 102122369 (Cynomolgus Monkey)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

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Citations for Mouse R-Spondin 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Female-Biased Expression of R-spondin 1 in Chicken Embryonic Gonads Is Estrogen-Dependent
    Authors: Mingde Zheng, Xikui Liu, Yu Meng, Xiao Lin, Jiahui Li, Jianguo Zhu et al.
    Animals (Basel)
  2. RSPOs facilitated HSC activation and promoted hepatic fibrogenesis
    Authors: X Yin, H Yi, L Wang, W Wu, X Wu, L Yu
    Oncotarget, 2016-09-27;7(39):63767-63778.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015-04-24;10(4):e0124213.
    Species: Mouse
    Sample Types: Cell Fraction, Cell Lysates, Whole Tissue
    Applications: Bioassay, IHC, Western Blot
  4. Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
    Authors: Saha S, Bhanja P, Kabarriti R
    PLoS ONE, 2011-09-15;6(9):e24072.
    Species: Mouse
    Sample Types: Serum
    Applications: Western Blot
  5. R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
    Authors: Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL
    Diabetologia, 2011-04-12;54(7):1726-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011-01-28;6(1):e16366.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Adult mouse fibroblasts retain organ-specific transcriptomic identity
    Authors: Forte, E;Ramialison, M;Nim, HT;Mara, M;Li, JY;Cohn, R;Daigle, SL;Boyd, S;Stanley, EG;Elefanty, AG;Hinson, JT;Costa, MW;Rosenthal, NA;Furtado, MB;
    eLife

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