Detects mouse Rae‑1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.
Monoclonal Rat IgG2A Clone # 205001
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
2.5 µg/106 cells
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.3-0.9 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of Recombinant Mouse NKG2D Fc Chimera (Catalog # 139-NK) to immobilized Recombinant Mouse Rae-1 epsilon Fc Chimera (Catalog # 1135-RA) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rae‑1 epsilon in BaF3 Mouse Cell Line Transfected with Mouse Rae-1 epsilon and eGFP by Flow Cytometry. BaF3 mouse pro-B cell line transfected with (A) mouse Rae-1 epsilon or (B) mouse Rae-1 alpha and eGFP was stained with Rat Anti-Mouse Rae‑1 epsilon Monoclonal Antibody (Catalog # MAB1135) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1 epsilon
Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig‑like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑7).
Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
Cerwenka, A. et al. (2000) Immunity 12:721.
Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
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