RAMP2 (receptor activity modifying protein 2) is a 20 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature mouse RAMP2 is a 145 amino acid (aa) type I transmembrane glycoprotein that contains a 115 aa extracellular domain (ECD) (aa 45-159) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, based on human, aa 100-106 are critical for AM binding. Over aa 45-159, mouse RAMP2 shares 57% and 83% aa identity with human and rat RAMP2, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant mouse RAMP2
Ser45-Val159
Accession # Q9WUP0
Ser45-Val159
Accession # Q9WUP0
Specificity
Detects mouse RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) RAMP2 is observed, and less than 1% cross-reactivity with rhRAMP1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Mouse RAMP2 Antibody
Detection of Mouse RAMP2 by Western Blot.
Western blot shows lysates of D3 mouse embryonic stem cell line. PVDF Membrane was probed with 1 µg/mL of Mouse RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6500) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP2 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 9.
Detection of RAMP2 in bEnd.3 Mouse Cell Line by Flow Cytometry.
bEnd.3 mouse endothelioma cell line was stained with Mouse RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6500, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).
Applications for Mouse RAMP2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line
Sample: bEnd.3 mouse endothelioma cell line
Western Blot
1 µg/mL
Sample: D3 mouse embryonic stem cell line
Sample: D3 mouse embryonic stem cell line
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: RAMP2
Long Name
Receptor Activity Modifying Protein 2
Alternate Names
calcitonin receptor-like receptor activity modifying protein 2, Calcitonin-receptor-like receptor activity-modifying protein 2, CRLR activity-modifying protein 2, receptor (calcitonin) activity modifying protein 2, receptor (G protein-coupled) activity modifying protein 2, receptor activity modifying protein 2, receptor activity-modifying protein 2, receptor-activity-modifying protein 2
Gene Symbol
RAMP2
UniProt
Additional RAMP2 Products
Product Documents for Mouse RAMP2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse RAMP2 Antibody
For research use only
Related Research Areas
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