Mouse/Rat Apolipoprotein H/ApoH Antibody

(1 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Apolipoprotein H/ApoH in Western blots and detects recombinant mouse Apolipoprotein H/ApoH in direct ELISAs. In direct ELISAs, approximately 40% cross-reactivity with recombinant human Apolipoprotein H/ApoH is observed.  
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Apolipoprotein H/ApoH
    Gly20-Cys345
    Accession # Q01339
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat and Mouse Apolipoprotein H/ApoH by Western Blot.

Western blot shows lysates of rat liver tissue and mouse serum. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse/Rat Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6575) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Apolipoprotein H/ApoH at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Apolipoprotein H/ApoH
Apolipoprotein H (ApoH), also known as beta 2‑Glycoprotein I/ beta 2-GPI, is a variably glycosylated member of the complement control superfamily of proteins with a molecular weight of aproximately 50 kDa (1, 2). Mature mouse ApoH consists of four tandem Sushi/SCR repeats followed by one Sushi-like repeat (3, 4). Mature mouse ApoH shares 76% and 42% aa sequence identity with human and rat ApoH, respectively. Hepatocyte-derived ApoH binds directly to negatively charged phospholipids (5). It circulates as a component of lipoprotein particles and as a lipid-free serum protein (6). ApoH also associates with liposomes and apoptotic cell debris, thereby enabling their renal clearance via Megalin uptake (7, 8). Circulating levels of ApoH are postively correlated with triglyceride-rich lipoprotein (VLDL) components in type II diabetes (9). ApoH inhibits thrombosis by blocking the activation of Coagulation Factor XI but also shows procoagulant activity by inhibiting the activation of Protein C (10, 11). ApoH can be cleaved by Plasmin at Lys317‑Thr318, an action that is enhanced by heparin (12, 13). ApoH cleavage reduces its ability to bind phospholipids and inhibit Factor XI activation but confers the ability to bind Plasminogen (10, 12, 14). Cleaved ApoH also demonstrates antiangiogenic activity, whereas intact ApoH does not (14). The production of antibodies against ApoH is a hallmark of Antiphospholipid Syndrome (APS), an autoimmune disorder that leads to hypercoagulability and recurrent miscarriages (15). ApoH binds to the surface antigen of Hepatitis B Virus and is associated with the development of HBV-induced hepatocellular carcinoma (6, 16).
  • References:
    1. Crook, M.A. et al. (2010) Atherosclerosis 209:32.
    2. Miyakis, S. et al. (2004) Thromb. Res. 114:335.
    3. Nonaka, M. et al. (1992) Genomics 13:1082.
    4. Lozier, J. et al. (1984) Proc. Natl. Acad. Sci. 81:3640.
    5. Wurm, H. (1984) Int. J. Biochem. 16:511.
    6. Mehdi, H. et al. (1994) J. Virol. 68:2415.
    7. Chonn, A. et al. (1995) J. Biol. Chem. 270:25845.
    8. Moestrup, S.K. et al. (1998) J. Clin. Invest. 102:902.
    9. Castro, A. et al. (2010) Atherosclerosis 209:201.
    10. Shi, T. et al. (2004) Proc. Natl. Acad. Sci. 101:3939.
    11. Mori, T. et al. (1996) Thromb. Haemost. 75:49.
    12. Hunt, J. et al. (1993) Proc. Natl. Acad. Sci. 90:2141.
    13. Guerin, J. et al. (2002) J. Biol. Chem. 277:2644.
    14. Sakai, T. et al. (2007) Am. J. Pathol. 171:1659.
    15. Adams, M. (2008) Semin. Thromb. Haemost. 34:251.
    16. Jing, X. et al. (2010) J. Cancer Res. Clin. Oncol. 16:1671.
  • Entrez Gene IDs:
    350 (Human); 11818 (Mouse); 287774 (Rat)
  • Alternate Names:
    Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Gram Negative Bacterial Inflammation Ameliorated by the Plasma Protein Beta 2-Glycoprotein I
    Sci Rep, 2016;6(0):33656.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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