Mouse/Rat cAMP Parameter Assay Kit

(5 citations)
(1 Review)
  
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Homogenates (50 uL), EDTA Plasma (50 uL), Urine (10 uL)
  • Sensitivity
    1.54 nmol/L
  • Assay Range
    3.8 - 240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)
  • Specificity
    Cyclic AMP in mouse and rat samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC53, Parameter Immunoassay Control Set 764 for Mouse/Rat cAMP - Please Inquire
Product Summary
The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean27.758.796.928.261.7102
Standard Deviation0.513.92.24.86.5
CV%1.81.747.87.86.4

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 100 98-103
Cell Lysates (n=2) 106 106-107
Mouse EDTA Plasma (n=4) 97 92-101
Rat EDTA Plasma (n=4) 104 101-108
Tissue Homogenates (n=4) 110 105-115
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat cAMP Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

    • Long Name
      Cyclic AMP
    • Alternate Names
      cAMP;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    5 Citations: Showing 1 - 5
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    Species
    Sample Type
    1. Molecular signaling underlying bulleyaconitine A (BAA)-induced microglial expression of prodynorphin
      Authors: TF Li, HY Wu, YR Wang, XY Li, YX Wang
      Sci Rep, 2017;7(0):45056.
      Species: Rat
      Sample Type: Cell Lysates
    2. Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice
      Sci Rep, 2016;6(0):30887.
      Species: Mouse
      Sample Type: Tissue Homogenates
    3. Neurofibromatosis type 1 alternative splicing is a key regulator of Ras signaling in neurons.
      Authors: Hinman M, Sharma A, Luo G, Lou H
      Mol Cell Biol, 2014;34(12):2188-97.
      Species: Mouse
      Sample Type: Cell Lysates
    4. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
      Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
      J Immunol, 2014;192(8):3765-77.
      Species: Rat
      Sample Type: Cell Lysates
    5. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
      Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
      Nat Commun, 2014;5(0):4725.
      Species: Mouse
      Sample Type: Tissue Homogenates
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    Parameter Immunoassay Control Set 764 for Mouse/Rat cAMP

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    Average Rating: 5 (Based on 1 review)

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    Sample TestedCHO Chinese hamster ovary cell line

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