The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
Cell Lysates (n=2)
Mouse EDTA Plasma (n=4)
Rat EDTA Plasma (n=4)
Tissue Homogenates (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.
Read what people are saying who have used Mouse/Rat cAMP Parameter Assay Kit.