Mouse/Rat cAMP Parameter Assay Kit

  (13 citations)
(1 Review)
    
Datasheet / CoA / SDS
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Homogenates (50 uL), EDTA Plasma (50 uL), Urine (10 uL)
  • Sensitivity
    1.54 nmol/L
  • Assay Range
    3.8 - 240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)
  • Specificity
    Cyclic AMP in mouse and rat samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC53 , Parameter Immunoassay Control Set 764 for Mouse/Rat cAMP - Please Inquire
Product Summary
The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 27.7 58.7 96.9 28.2 61.7 102
Standard Deviation 0.5 1 3.9 2.2 4.8 6.5
CV% 1.8 1.7 4 7.8 7.8 6.4

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 100 98-103
Cell Lysates (n=2) 106 106-107
Mouse EDTA Plasma (n=4) 97 92-101
Rat EDTA Plasma (n=4) 104 101-108
Tissue Homogenates (n=4) 110 105-115
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 cAMP [Biotin]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

  • Long Name:
    Cyclic AMP
  • Alternate Names:
    cAMP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Molecular mechanisms of calcium signaling in the modulation of small intestinal ion transports and bicarbonate secretion
    Authors: X Yang, G Wen, B Tuo, F Zhang, H Wan, J He, S Yang, H Dong
    Oncotarget, 2018;9(3):3727-3740.
    Species: Mouse
    Sample Type: Protein
  2. Gq activity- and ?-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility
    Authors: DL Zhang, YJ Sun, ML Ma, YJ Wang, H Lin, RR Li, ZL Liang, Y Gao, Z Yang, DF He, A Lin, H Mo, YJ Lu, MJ Li, W Kong, KY Chung, F Yi, JY Li, YY Qin, J Li, ARB Thomsen, AW Kahsai, ZJ Chen, ZG Xu, M Liu, D Li, X Yu, JP Sun
    Elife, 2018;7(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  3. Molecular signaling underlying bulleyaconitine A (BAA)-induced microglial expression of prodynorphin
    Authors: TF Li, HY Wu, YR Wang, XY Li, YX Wang
    Sci Rep, 2017;7(0):45056.
    Species: Rat
    Sample Type: Cell Lysates
  4. Exendin-4, a glucagon-like peptide-1 analogue accelerates healing of chronic gastric ulcer in diabetic rats
    Authors: YC Chen, CC Ho, CH Yi, XZ Liu, TT Cheng, CF Lam
    PLoS ONE, 2017;12(11):e0187434.
    Species: Rat
    Sample Type: Tissue Homogenates
  5. Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells
    Authors: R Shi, ZT Xiao, YJ Zheng, YL Zhang, JW Xu, JH Huang, WL Zhou, PB Li, WW Su
    Cell. Physiol. Biochem., 2017;44(3):1146-1160.
    Species: Rat
    Sample Type: Cell Culture Supernates
  6. Targeting G-protein coupled receptor-related signaling pathway in a murine xenograft model of appendiceal pseudomyxoma peritonei
    Authors: AK Dilly, BD Honick, YJ Lee, ZS Guo, HJ Zeh, DL Bartlett, HA Choudry
    Oncotarget, 2017;8(63):106888-106900.
    Species: Mouse
    Sample Type: Tissue Homogenates
  7. p38? MAPK signaling mediates exenatide-stimulated microglial ?-endorphin expression
    Authors: HY Wu, XF Mao, H Fan, YX Wang
    Mol. Pharmacol, 2017;0(0):.
    Species: Rat
    Sample Type: Cell Lysates
  8. Increased efficacy of a dendritic cell-based therapeutic cancer vaccine with adenosine receptor antagonist and CD73 inhibitor
    Authors: S Arab, N Kheshtchin, M Ajami, M Ashurpoor, A Safvati, A Namdar, R Mirzaei, N Mousavi Ni, F Jadidi-Nia, MH Ghahremani, J Hadjati
    Tumour Biol, 2017;39(3):1010428317695.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  9. Effects of Aluminium on long-term Memory in Rats and on SIRT1 Mediating the Transcription of CREB-Dependent Gene in Hippocampus
    Authors: D Yan, C Jin, Y Cao, L Wang, X Lu, J Yang, S Wu, Y Cai
    Basic Clin. Pharmacol. Toxicol., 2017;0(0):.
    Species: Rat
    Sample Type: Tissue Homogenates
  10. Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice
    Sci Rep, 2016;6(0):30887.
    Species: Mouse
    Sample Type: Tissue Homogenates
  11. Neurofibromatosis type 1 alternative splicing is a key regulator of Ras signaling in neurons.
    Authors: Hinman M, Sharma A, Luo G, Lou H
    Mol Cell Biol, 2014;34(12):2188-97.
    Species: Mouse
    Sample Type: Cell Lysates
  12. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
    Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
    J Immunol, 2014;192(8):3765-77.
    Species: Rat
    Sample Type: Cell Lysates
  13. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
    Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
    Nat Commun, 2014;5(0):4725.
    Species: Mouse
    Sample Type: Tissue Homogenates
Expand to show all Citations

FAQs

No product specific FAQs exist for this product, however you may

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Parameter Immunoassay Control Set 764 for Mouse/Rat cAMP

Ctrl QC53
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 1 review)

Have you used Mouse/Rat cAMP Parameter Assay Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse/Rat cAMP Parameter Assay Kit.

Filter by Ratings
Excellent
(1)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
  Excellent
  Anonymous 07/24/2017
Show

Summary

Sample Tested CHO Chinese hamster ovary cell line

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee