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Mouse/Rat cAMP Parameter Assay Kit

R&D Systems | Catalog # KGE012B

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Key Product Details

Assay Length

3.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Tissue Homogenates (50 µL), EDTA Plasma (50 µL), Urine (10 µL)

Sensitivity

1.54 nmol/L

Assay Range

3.75-240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)
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Product Summary for Mouse/Rat cAMP Parameter Assay Kit

The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Mouse, Rat

Specificity

Cyclic AMP in mouse and rat samples

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Tissue Homogenates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (nmol/L) 27.7 58.7 96.9 28.2 61.7 102
Standard Deviation 0.5 1.0 3.9 2.2 4.8 6.5
CV% 1.8 1.7 4.0 7.8 7.8 6.4

Recovery for Mouse/Rat cAMP Parameter Assay Kit

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 100 98-103
Cell Lysates (n=2) 106 106-107
Mouse EDTA Plasma (n=4) 97 92-101
Rat EDTA Plasma (n=4) 104 101-108
Tissue Homogenates (n=4) 110 105-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Mouse/Rat cAMP ELISA Linearity

Scientific Data Images for Mouse/Rat cAMP Parameter Assay Kit

Mouse/Rat cAMP ELISA Standard Curve

Mouse/Rat cAMP ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: cAMP

cAMP (adenosine 3’5’-cyclic monophosphate) is a ubiquitous intracellular second messenger that mediates a diverse range of cellular processes in all organisms from bacteria to higher eukaryotes. It is converted from adenosine triphosphate (ATP) by adenylyl cyclases (ACs), and is inactivated by phosphodiesterases (PDEs) which catalyze its hydrolysis to 5’-AMP. In mammals, upon interaction with extracellular ligands, G protein coupled receptors (GPCRs) linked to Gαs activate the family of nine transmembrane ACs to increase intracellular cAMP. In contrast, GPCRs associated with Gαi/o inhibit the synthesis of cAMP by transmembrane ACs. cAMP binds and activates protein kinase A (PKA), the guanine nucleotide exchange factors Epac1/2, and cyclic nucleotide-gated ion channels. It can be released extracellularly where it is converted to adenosine by phosphodiesterases and nucleotidases.

Long Name

Cyclic AMP

Alternate Names

Cyclic AMP

Additional cAMP Products

Product Documents for Mouse/Rat cAMP Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse/Rat cAMP Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Regulatory T Cells (Tregs)

Citations for Mouse/Rat cAMP Parameter Assay Kit

Customer Reviews for Mouse/Rat cAMP Parameter Assay Kit (2)

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  • Mouse/Rat cAMP Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Kidney tissue
    Verified Customer | Posted 03/30/2023
  • Mouse/Rat cAMP Parameter Assay Kit
    Name: Anonymous
    Sample Tested: CHO Chinese hamster ovary cell line
    Verified Customer | Posted 07/24/2017

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