|Intra-Assay Precision||Inter-Assay Precision|
The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Samples (n=4)||100||98-103|
|Cell Lysates (n=2)||106||106-107|
|Mouse EDTA Plasma (n=4)||97||92-101|
|Rat EDTA Plasma (n=4)||104||101-108|
|Tissue Homogenates (n=4)||110||105-115|
Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.
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