Mouse/Rat CD31/PECAM-1 Alexa Fluor® 488-conjugated Antibody

(9 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
    Glu18-Lys590
    Accession # Q08481
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM‑1 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD31/PECAM-1

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  • References:
    1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
    2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
    3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
    4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
    5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
    6. Ilan, N. et al. (2001) FASEB J. 15:362.
    7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
    8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
    9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
    10. Mamdouh, Z. et al. (2003) Nature 421:748.
    11. Gao, C. et al. (2003) Blood 102:169.
    12. Falati, S. et al. (2006) Blood 107:535.
    13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
    14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis
    Authors: S Kijani, AM Vázquez, M Levin, J Borén, P Fogelstran
    Physiol Rep, 2017;5(14):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017;19(3):189-201.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Age-dependent modulation of vascular niches for haematopoietic stem cells
    Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
    Nature, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Age-dependent modulation of vascular niches for haematopoietic stem cells
    Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
    Nature, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Oral mucosa harbors a high frequency of endothelial cells - a novel postnatal cell source for angiogenic regeneration
    Stem Cells Dev., 2016;0(0):.
    Species: Rat
    Sample Type: Whole Cells
    Application: Flow
  6. Blood flow controls bone vascular function and osteogenesis
    Nat Commun, 2016;7(0):13601.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Ctyometry
  7. Filter-Dense Multicolor Microscopy.
    Authors: Kijani S, Yrlid U, Heyden M, Levin M, Boren J, Fogelstrand P
    PLoS ONE, 2015;10(3):e0119499.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  8. Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
    Authors: Ramasamy S, Kusumbe A, Wang L, Adams R
    Nature, 2014;507(7492):376-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014;507(7492):323-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Alexa Fluor® 488-conjugated Control

Ctrl IC108G
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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