Mouse CD31/PECAM-1 Biotinylated Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD31/PECAM‑1 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM‑1
    Glu18-Lys590
    Accession # Q08481
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse CD31/PECAM‑1 (Catalog # 3628-PC)
  • Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse CD31/PECAM‑1 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3628, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD31/PECAM-1

PECAM-1 (platelet-endothelial cell adhesion molecule-1; also known as CD31) is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for transendothelial migration (TEM) (1-3). The extracellular domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

  • References:
    1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
    2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
    3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
    4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
    5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
    6. Ilan, N. et al. (2001) FASEB J. 15:362.
    7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
    8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
    9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
    10. Mamdouh, Z. et al. (2003) Nature 421:748.
    11. Gao, C. et al. (2003) Blood 102:169.
    12. Falati, S. et al. (2006) Blood 107:535.
    13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
    14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
  • Long Name:
    Platelet Endothelial Cell Adhesion Molecule 1
  • Entrez Gene IDs:
    5175 (Human); 18613 (Mouse); 29583 (Rat)
  • Alternate Names:
    adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; endoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Strategies to Mitigate Variability in Engineering Human Nasal Cartilage
    Authors: SHJ Andrews, M Kunze, A Mulet-Sier, L Williams, K Ansari, M Osswald, AB Adesida
    Sci Rep, 2017;7(1):6490.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Transcriptional Regulation of Cystathionine-gamma-Lyase in Endothelial Cells by NADPH Oxidase 4-Dependent Signaling.
    Authors: Mistry R, Murray T, Prysyazhna O, Martin D, Burgoyne J, Santos C, Eaton P, Shah A, Brewer A
    J Biol Chem, 2016;291(4):1774-88.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Clustering
  3. Filter-Dense Multicolor Microscopy.
    Authors: Kijani S, Yrlid U, Heyden M, Levin M, Boren J, Fogelstrand P
    PLoS ONE, 2015;10(3):e0119499.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Biotinylated Control

Ctrl BAF108 19
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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