Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB3628A-100
FAB3628A-025
Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse CD31/PECAM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human CD31 and recombinant porcine CD31 is observed. Detects mouse CD31 and rat CD31 in flow cytometry.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1
Glu18-Lys590
Accession # Q08481
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD31/PECAM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CD31/PECAM-1 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CD31/PECAM‑1 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Goat Anti-Mouse/Rat CD31/PECAM-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3628A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mouse CD31/PECAM-1 by Flow Cytometry View Larger

Detection of Mouse CD31/PECAM-1 by Flow Cytometry CD31hiEmcnhi vessel and bone formation decreased during ageing but increased in bone regeneration. A, Representative images of CD31 (green), EMCN (red) and Osterix (white) immunostaining. Scale bar, 100 μm. G, growth plate. B, Quantification of CD31 and EMCN positive vessel volume in distal femora. C, Quantitative analysis of Osterix‐positive (OSX+) osteoprogenitors in distal femora. D and E, FACS analysis dot plot (D) and quantification (E) of CD31hiEMCNhi ECs. F, Representative images of CD31 (green) and EMCN (red) immunostaining in bone regeneration area after femoral trabecular bone ablation. Nuclei, DAPI (blue). Scale bar, 100 μm. G and H, Representative μCT images (G) and quantitative μCT analysis (H) of bone regeneration after femoral trabecular bone ablation. Selected areas for the measurements of bone volume (BV)/tissue volume (TV) were indicated with a yellow square. Data are shown as mean ± SD, (n = 6 in B, C, E and H). *P < .05; **P < .01 by one‐way ANOVA Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32080957), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD31/PECAM-1

PECAM-1 (Platelet-Endothelial Cell Adhesion Molecule-1), also known as CD31, is a 130 kDa type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily (1, 2). Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 is concentrated at cell-cell junctions and is required for Transendothelial Migration (TEM) (1-3). The Extracellular Domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation (3, 4). The cytoplasmic domain contains Immunoregulatory Tyrosine-based Inhibitory and Switch Motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules (1, 5). Metalloproteinase-mediated ectodomain shedding occurs during apoptosis (6) but increased serum PECAM-1 ectodomain in HIV and active multiple sclerosis occurs independent of apoptosis (7, 8). In humans, expression of six isoforms with exon deletions in the cytoplasmic domain is tissue- and stage-specific, but full-length PECAM-1 is predominant. A form lacking the ITSM predominates in mouse (9). Mouse PECAM-1 ECD shows 77%, 63%, 63%, 63%, and 61% amino acid (aa) identity with rat, human, canine, porcine, and bovine PECAM-1, respectively. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions (3, 10). Homotypic adhesion and signaling functions also strongly suppress mitochondria-dependent apoptosis (11). In platelets, PECAM-1 is necessary for limiting thrombus formation (12) and promoting integrin-mediated clot retraction and platelet spreading (13), but mechanisms for these phenomena are unclear. PECAM-/- mice are deficient in chemokine-mediated chemotaxis (14).

References
  1. Ilan, N. and J.A. Madri (2003) Curr. Opin. Cell Biol. 15:515.
  2. Xie, Y. and W.A. Muller (1993) Proc. Natl. Acad. Sci. USA 90:5569.
  3. Liao, F. et al. (1997) J. Exp. Med. 185:1349.
  4. Nakada, M.T. et al. (2000) J. Immunol. 164:452.
  5. Chemnitz, J.M. et al. (2004) J. Immunol. 173:945.
  6. Ilan, N. et al. (2001) FASEB J. 15:362.
  7. Eugenin, E.A. et al. (2006) J. Leukoc. Biol. 79:444.
  8. Losy, J. et al. (1999) J. Neuroimmunol. 99:169.
  9. Wang, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284:H1008.
  10. Mamdouh, Z. et al. (2003) Nature 421:748.
  11. Gao, C. et al. (2003) Blood 102:169.
  12. Falati, S. et al. (2006) Blood 107:535.
  13. Wee, J.L. and D.E. Jackson (2005) Blood 106:3816.
  14. Wu, Y. et al. (2005) J. Immunol. 175:3484.
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Entrez Gene IDs
5175 (Human); 18613 (Mouse); 29583 (Rat)
Alternate Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule

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Citations for Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. miR-188-3p targets skeletal endothelium coupling of angiogenesis and osteogenesis during ageing
    Authors: WZ He, M Yang, Y Jiang, C He, YC Sun, L Liu, M Huang, YR Jiao, KX Chen, J Hou, M Huang, YL Xu, X Feng, Y Liu, Q Guo, H Peng, Y Huang, T Su, Y Xiao, Y Li, C Zeng, G Lei, XH Luo, CJ Li
    Cell Death & Disease, 2022-05-25;13(5):494.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis
    Authors: X Liu, Y Chai, G Liu, W Su, Q Guo, X Lv, P Gao, B Yu, G Ferbeyre, X Cao, M Wan
    Nature Communications, 2021-03-23;12(1):1832.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MiR-497?195 cluster regulates angiogenesis during coupling with osteogenesis by maintaining endothelial Notch and HIF-1? activity
    Authors: M Yang, CJ Li, X Sun, Q Guo, Y Xiao, T Su, ML Tu, H Peng, Q Lu, Q Liu, HB He, TJ Jiang, MX Lei, M Wan, X Cao, XH Luo
    Nat Commun, 2017-07-07;8(0):16003.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
    Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
    Nat. Cell Biol, 2017-02-20;19(3):189-201.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Age-dependent modulation of vascular niches for haematopoietic stem cells
    Authors: AP Kusumbe, SK Ramasamy, T Itkin, MA Mäe, UH Langen, C Betsholtz, T Lapidot, RH Adams
    Nature, 2016-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone.
    Authors: Kusumbe A, Ramasamy S, Adams R
    Nature, 2014-03-12;507(7492):323-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody

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Mouse/Rat CD31/PECAM-1 APC-conjugated Antibody
By Anonymous on 04/29/2021
Application: Flow
Reason for Rating: Works excellent in the mouse bone marrow cells. Sample Tested: Bone marrow cells Species: Mouse