Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 485422
View all CREB Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse CREB
Met1-Lys69
Accession # NP_001032815

Specificity

The antibody detects endogenous mouse and rat CREB on Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse/Rat CREB Antibody

Detection of Mouse/Rat CREB antibody by Western Blot.

Detection of Mouse/Rat CREB by Western Blot.

Western blot shows lysates of A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse/Rat CREB Monoclonal Antibody (Catalog # MAB5435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Mouse/Rat CREB Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 2 using MAB5435 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CREB

The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP family of transcription factors. Each bZIP family member contains a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. CREB becomes phosphorylated at specific residues in response to DNA damage and circadian cycles.

Long Name

cAMP Response Element-binding Protein

Alternate Names

CREB1

Entrez Gene IDs

1385 (Human); 12912 (Mouse)

Gene Symbol

CREB1

UniProt

NP_001032815

Additional CREB Products

Product Documents for Mouse/Rat CREB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat CREB Antibody

For research use only

Customer Reviews for Mouse/Rat CREB Antibody (1)

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  • Mouse/Rat CREB Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Lung single-cell suspension
    Species: Mouse
    Verified Customer | Posted 06/06/2019
    Mouse/Rat CREB Antibody MAB5435
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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