Mouse/Rat CREB Antibody

Catalog # Availability Size / Price Qty
MAB5435
MAB5435-SP
Detection of Mouse/Rat CREB by Western Blot.
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Mouse/Rat CREB Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
The antibody detects endogenous mouse and rat CREB on Western blots.
Source
Monoclonal Rat IgG2A Clone # 485422
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CREB
Met1-Lys69
Accession # NP_001032815
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse/Rat CREB by Western Blot. View Larger

Detection of Mouse/Rat CREB by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse/Rat CREB Monoclonal Antibody (Catalog # MAB5435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CREB

The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP family of transcription factors. Each bZIP family member contains a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. CREB becomes phosphorylated at specific residues in response to DNA damage and circadian cycles.

Long Name
cAMP Response Element-binding Protein
Entrez Gene IDs
1385 (Human); 12912 (Mouse)
Alternate Names
active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein

Product Datasheets

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