The cAMP response element binding protein (CREB), also known as CREB1, belongs to the bZIP family of transcription factors. Each bZIP family member contains a basic domain that mediates DNA binding and a leucine zipper domain that facilitates dimerization. Within the promoter of target genes, CREB dimers bind cAMP response elements, defined by the palindromic consensus sequence TGACGTCA. CREB becomes phosphorylated at specific residues in response to DNA damage and circadian cycles.
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 485422
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Product Specifications
Immunogen
E. coli-derived recombinant mouse CREB
Met1-Lys69
Accession # NP_001032815
Met1-Lys69
Accession # NP_001032815
Specificity
The antibody detects endogenous mouse and rat CREB on Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse/Rat CREB Antibody
Detection of Mouse/Rat CREB by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse/Rat CREB Monoclonal Antibody (Catalog # MAB5435) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CREB at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Mouse/Rat CREB Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line
Sample: A20 mouse B cell lymphoma cell line and M1 mouse myeloid leukemia cell line
Reviewed Applications
Read 1 review rated 2 using MAB5435 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CREB
Long Name
cAMP Response Element-binding Protein
Alternate Names
CREB1
Gene Symbol
CREB1
UniProt
Additional CREB Products
Product Documents for Mouse/Rat CREB Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat CREB Antibody
For research use only
Customer Reviews for Mouse/Rat CREB Antibody (1)
2 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Lung single-cell suspensionSpecies: MouseVerified Customer | Posted 06/06/2019
Bio-Techne ResponseThank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
TGF-beta Signaling Pathways
VEGF - VEGF R2 Signaling Pathways
