Mouse/Rat Cystatin C Quantikine ELISA Kit

(13 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
  • Sensitivity
    12.9 pg/mL
  • Assay Range
    125.0 - 8,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant mouse and rat Cystatin C
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat Cystatin C Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Cystatin C in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse Cystatin C and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse or rat Cystatin C showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse and rat Cystatin C.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean43489430654659423257
Standard Deviation14.929.285.943.551.2213
CV%3.43.32.89.45.46.5

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean43489430654659423257
Standard Deviation14.929.285.943.551.2213
CV%3.43.32.89.45.46.5

Recovery

The recovery of Cystatin C spiked into cell culture media was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 98-113
Linearity
To assess the linearity of the assay, samples containing high concentrations of mouse or rat Cystatin C were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat Cystatin C Quantikine ELISA Kit
Mouse/Rat Cystatin C Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Cystatin C
Cystatin C is a secreted cysteine protease inhibitor that inhibits Cathepsins B, H, K, L, and S. Cystatin C serum concentration correlates closely to the glomerular filtration rate (GFR); elevated levels are associated with coronary artery and cardiovascular disease risk. Dysregulation of Cystatin C can modulate tumor growth and metastasis. In humans, the L68Q variant forms dimers and oligomers more easily than wild type protein and is the cause for hereditary Cystatin C amyloid angiopathy.
    • Entrez Gene IDs
      1471 (Human); 13010 (Mouse); 25307 (Rat);
    • Alternate Names
      ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    13 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Dexamethasone Modifies Cystatin C-Based Diagnosis of Acute Kidney Injury During Cisplatin-Based Chemotherapy
      Authors: TJ Pianta, JW Pickering, L Succar, M Chin, T Davidson, NA Buckley, F Mohamed, ZH Endre
      Kidney Blood Press. Res, 2017;42(1):62-75.
      Species: Rat
      Sample Type: Urine
    2. A peptide vaccine targeting angiotensin II attenuates the cardiac dysfunction induced by myocardial infarction
      Authors: R Watanabe, JI Suzuki, K Wakayama, Y Maejima, M Shimamura, H Koriyama, H Nakagami, H Kumagai, Y Ikeda, H Akazawa, R Morishita, I Komuro, M Isobe
      Sci Rep, 2017;7(0):43920.
      Species: Rat
      Sample Type: Serum
    3. Prenatal hypoxia leads to hypertension, renal renin-angiotensin system activation and exacerbates salt-induced pathology in a sex-specific manner
      Authors: SL Walton, H Bielefeldt, RR Singh, J Li, TM Paravicini, MH Little, KM Moritz
      Sci Rep, 2017;7(1):8241.
      Species: Mouse
      Sample Type: Plasma
    4. Validation of a routine two-sample iohexol plasma clearance assessment of GFR and an evaluation of common endogenous markers in a rat model of CKD
      Authors: ME Turner, KJ Laverty, PS Jeronimo, M Kaufmann, G Jones, CA White, RM Holden, MA Adams
      Physiol Rep, 2017;5(9):.
      Species: Rat
      Sample Type: Plasma
    5. Megalin dependent urinary cystatin C excretion in ischemic kidney injury in rats
      Authors: D Jensen, C Kierulf-La, MLV Kristensen, R Nørregaard, K Weyer, R Nielsen, EI Christense, H Birn
      PLoS ONE, 2017;12(6):e0178796.
      Species: Mouse
      Sample Type: Plasma
    6. Effect of Antifibrotic MicroRNAs Crosstalk on the Action of N-acetyl-seryl-aspartyl-lysyl-proline in Diabetes-related Kidney Fibrosis
      Sci Rep, 2016;6(0):29884.
      Species: Mouse
      Sample Type: Plasma
    7. Beneficial Effects of Sarpogrelate and Rosuvastatin in High Fat Diet/Streptozotocin-Induced Nephropathy in Mice
      Authors: DH Kim, BH Choi, SK Ku, JH Park, E Oh, MK Kwak
      PLoS ONE, 2016;11(4):e0153965.
      Species: Mouse
      Sample Type: Urine
    8. Obesity-induced kidney injury is attenuated by amelioration of aberrant PHD2 activation in proximal tubules
      Sci Rep, 2016;6(0):36533.
      Species: Mouse
      Sample Type: Urine
    9. Discordance in cathepsin B and cystatin C expressions in bronchoalveolar fluids between murine bleomycin-induced fibrosis and human idiopathic fibrosis
      Respir Res, 2016;17(1):118.
      Species: Mouse
      Sample Type: Tissue Homogenates
    10. Aggravated Cardiac Remodeling post Aortocaval Fistula in Unilateral Nephrectomized Rats.
      Authors: Wu J, Cheng Z, Gu Y, Zou W, Zhang M, Zhu P, Hu S
      PLoS ONE, 2015;10(8):e0134579.
      Species: Rat
      Sample Type: Plasma
    11. An Atherogenic Paigen-Diet Aggravates Nephropathy in Type 2 Diabetic OLETF Rats.
      Authors: Nozako M, Koyama T, Nagano C, Sato M, Matsumoto S, Mitani K, Yasufuku R, Kohashi M, Yoshikawa T
      PLoS ONE, 2015;10(11):e0143979.
      Species: Rat
      Sample Type: Urine
    12. Interactions of DPP-4 and integrin beta1 influences endothelial-to-mesenchymal transition.
      Authors: Shi S, Srivastava S, Kanasaki M, He J, Kitada M, Nagai T, Nitta K, Takagi S, Kanasaki K, Koya D
      Kidney Int, 2015;88(3):479-89.
      Species: Mouse
      Sample Type: Plasma
    13. Intrarenal resistance index for the assessment of acute renal injury in a rat liver transplantation model.
      Authors: Kong H, Chen F, He Y, Wu L, Wang L, Zhu S, Zheng S
      BMC Nephrol, 2013;14(0):55.
      Species: Rat
      Sample Type: Serum
    Expand to show all 13 Citations
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