Mouse/Rat DARPP-32 Antibody

Catalog # Availability Size / Price Qty
MAB4230
MAB4230-SP
Detection of Mouse/Rat DARPP‑32 by Western Blot.
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Product Details
Citations (8)
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Mouse/Rat DARPP-32 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
Source
Monoclonal Rat IgG2A Clone # 375604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DARPP-32
Ala51-Thr204
Accession # Q9UD71.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed paraffin-embedded sections of rat brain (corpus striatum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Mouse/Rat DARPP‑32 antibody by Western Blot. View Larger

Detection of Mouse/Rat DARPP‑32 by Western Blot. Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name
Dopamine and cAMP-regulated Phosphoprotein
Entrez Gene IDs
84152 (Human); 19049 (Mouse); 360616 (Rat)
Alternate Names
DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Product Datasheets

Citations for Mouse/Rat DARPP-32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats
    Authors: M Derbis, E Kul, D Niewiadoms, M Sekrecki, A Piasecka, K Taylor, RK Hukema, O Stork, K Sobczak
    Nature Communications, 2021;12(1):1265.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease
    Authors: ME Schmidt, NS Caron, AE Aly, FL Lemarié, L Dal Cengio, Y Ko, N Lazic, L Anderson, B Nguyen, LA Raymond, MR Hayden
    Front Cell Neurosci, 2020;14(0):590569.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival
    Authors: CJ Burrus, SU McKinstry, N Kim, MI Ozlu, AV Santoki, FY Fang, A Ma, YB Karadeniz, AK Worthingto, I Dragatsis, S Zeitlin, HH Yin, C Eroglu
    Cell Rep, 2020;30(3):642-657.e6.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  4. Potent and sustained huntingtin lowering via AAV5 encoding miRNA preserves striatal volume and cognitive function in a humanized mouse model of Huntington disease
    Authors: NS Caron, AL Southwell, CC Brouwers, LD Cengio, Y Xie, HF Black, LM Anderson, S Ko, X Zhu, SJ van Devent, MM Evers, P Konstantin, MR Hayden
    Nucleic Acids Res., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease
    Authors: ME Schmidt, C Buren, JP Mackay, D Cheung, L Dal Cengio, LA Raymond, MR Hayden
    BMC Biol., 2018;16(1):58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease
    Authors: R Gomez-Past, ET Burchfiel, DW Neef, AM Jaeger, E Cabiscol, SU McKinstry, A Doss, A Aballay, DC Lo, SS Akimov, CA Ross, C Eroglu, DJ Thiele
    Nat Commun, 2017;8(0):14405.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.
    Authors: Chen, Meng, Wan, Yehong, Ade, Kristen, Ting, Jonathan, Feng, Guoping, Calakos, Nicole
    J Neurosci, 2011;31(26):9563-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  8. Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling.
    Authors: Teegarden SL, Scott AN, Bale TL
    Neuroscience, 2009;162(4):924-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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