Mouse/Rat DARPP-32 Antibody
Mouse/Rat DARPP-32 Antibody Summary
Ala51-Thr204
Accession # Q9UD71.2
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse/Rat DARPP‑32 by Western Blot. Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DARPP-32
Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.
Product Datasheets
Citations for Mouse/Rat DARPP-32 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 9
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Regional vulnerability in a neurodegenerative disease: Delineating SCA1 CNS and muscle therapeutic targets using a conditional mutant ATXN1 mouse
Authors: L Duvick, WM Southern, K Benzow, HP Handler, JS Mitchell, H Kuivinen, UK Gadiparthi, P Yang, A Soles, C Scheeler, O Rainwater, S Shannah, E Larson, T Nichols-Me, Y You, B Oâ Callagh, HY Zoghbi, JM Ervasti, M Cvetanovic, MD Koob, HT Orr
bioRxiv : the preprint server for biology, 2023-04-11;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats
Authors: M Derbis, E Kul, D Niewiadoms, M Sekrecki, A Piasecka, K Taylor, RK Hukema, O Stork, K Sobczak
Nature Communications, 2021-02-24;12(1):1265.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease
Authors: ME Schmidt, NS Caron, AE Aly, FL Lemarié, L Dal Cengio, Y Ko, N Lazic, L Anderson, B Nguyen, LA Raymond, MR Hayden
Front Cell Neurosci, 2020-11-05;14(0):590569.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival
Authors: CJ Burrus, SU McKinstry, N Kim, MI Ozlu, AV Santoki, FY Fang, A Ma, YB Karadeniz, AK Worthingto, I Dragatsis, S Zeitlin, HH Yin, C Eroglu
Cell Rep, 2020-01-21;30(3):642-657.e6.
Species: Mouse
Sample Types: Tissue
Applications: IHC -
Potent and sustained huntingtin lowering via AAV5 encoding miRNA preserves striatal volume and cognitive function in a humanized mouse model of Huntington disease
Authors: NS Caron, AL Southwell, CC Brouwers, LD Cengio, Y Xie, HF Black, LM Anderson, S Ko, X Zhu, SJ van Devent, MM Evers, P Konstantin, MR Hayden
Nucleic Acids Res., 2020-01-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease
Authors: ME Schmidt, C Buren, JP Mackay, D Cheung, L Dal Cengio, LA Raymond, MR Hayden
BMC Biol., 2018-06-27;16(1):58.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease
Authors: R Gomez-Past, ET Burchfiel, DW Neef, AM Jaeger, E Cabiscol, SU McKinstry, A Doss, A Aballay, DC Lo, SS Akimov, CA Ross, C Eroglu, DJ Thiele
Nat Commun, 2017-02-13;8(0):14405.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.
Authors: Chen, Meng, Wan, Yehong, Ade, Kristen, Ting, Jonathan, Feng, Guoping, Calakos, Nicole
J Neurosci, 2011-06-29;31(26):9563-73.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling.
Authors: Teegarden SL, Scott AN, Bale TL
Neuroscience, 2009-05-22;162(4):924-32.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
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