Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 375604
View all DARPP-32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DARPP-32
Ala51-Thr204
Accession # Q9UD71.2

Specificity

Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse/Rat DARPP‑32 Antibody

Detection of Mouse/Rat DARPP‑32 antibody by Western Blot.

Detection of Mouse/Rat DARPP‑32 by Western Blot.

Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse/Rat DARPP‑32 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat brain (corpus striatum)

Western Blot

1 µg/mL
Sample: Mouse brain corpus striatum tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Q9UD71.2

Additional DARPP-32 Products

Product Documents for Mouse/Rat DARPP‑32 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse/Rat DARPP‑32 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for Mouse/Rat DARPP‑32 Antibody

Customer Reviews for Mouse/Rat DARPP‑32 Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat DARPP‑32 Antibody and earn rewards!

Have you used Mouse/Rat DARPP‑32 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies