Mouse/Rat Draxin Alexa Fluor® 488-conjugated Antibody

Catalog #: AF6149G Datasheet / COA / SDS

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AF6149G-100UG

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Mouse/Rat Draxin Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse, Rat

Specificity

Detects mouse and rat Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Draxin is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Draxin
Gly26-Ile343
Accession # Q6PAL1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Draxin

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 268-327). Mature mouse Draxin (aa 26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.

Long Name

Dorsal Repulsive Axon Guidance Protein

Entrez Gene IDs

374946 (Human); 70433 (Mouse); 691317 (Rat)

Alternate Names

AGPA3119; C1orf187; chromosome 1 open reading frame 187; dorsal inhibitory axon guidance protein; Dorsal repulsive axon guidance protein; Draxin; FLJ34999; MGC117222; Neucrin; UNQ3119