Mouse/Rat Draxin Antibody

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Detection of Rat Draxin by Western Blot.
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Product Details
Citations (1)
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Mouse/Rat Draxin Antibody Summary

Species Reactivity
Mouse, Rat
Detects mouse and rat Draxin in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Draxin is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Draxin
Accession # Q6PAL1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Draxin antibody by Western Blot. View Larger

Detection of Rat Draxin by Western Blot. Western blot shows lysates of rat embryonic hippocampal glial cells. PVDF Membrane was probed with 1 µg/mL of Mouse/Rat Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Draxin at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry Draxin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Draxin in Mouse Embryo. Draxin was detected in immersion fixed frozen sections of mouse embryo (E13) using Mouse Draxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6149) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cells in the midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Draxin

Draxin (Dorsal repulsive axon guidance protein; also neucrin) is a secreted, 58 kDa, presumable glycoprotein member of the Draxin family of molecules. In mammals, it is expressed in neurons (axons), astroglia, and likely cells of the developing somite. Draxin acts as a Wnt antagonist, apparently by binding to LRP6. The net effect is to block neural crest migration, and the organization of axons into functional tracts or bundles (fasciculation). Mature mouse Draxin is 318 amino acids (aa) in length. It contains one potential N-linked glycosylation site, followed by a Cys-rich domain (aa 268-327). Mature mouse Draxin (aa 26-343) shares 80% aa identity with mature human Draxin, and 94% aa identity with mature rat Draxin.

Long Name
Dorsal Repulsive Axon Guidance Protein
Entrez Gene IDs
374946 (Human); 70433 (Mouse); 691317 (Rat)
Alternate Names
AGPA3119; C1orf187; chromosome 1 open reading frame 187; dorsal inhibitory axon guidance protein; Dorsal repulsive axon guidance protein; Draxin; FLJ34999; MGC117222; Neucrin; UNQ3119

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Citation for Mouse/Rat Draxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. DRAXIN regulates interhemispheric fissure remodelling to influence the extent of corpus callosum formation
    Authors: L Morcom, TJ Edwards, E Rider, D Jones-Davi, JW Lim, KS Chen, RJ Dean, J Bunt, Y Ye, I Gobius, R Suárez, S Mandelstam, EH Sherr, LJ Richards
    Elife, 2021-05-04;10(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot


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