Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit

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MFK00
Mouse/Rat Flt-3 Ligand ELISA Standard Curve
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Citations (24)
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Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
5 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse Flt-3 Ligand and natural rat Flt-3 Ligand
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat Flt-3 Ligand Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat Flt-3 Ligand levels in cell culture supernates and serum. It contains NS0-expressed recombinant mouse Flt-3 Ligand and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse or rat Flt-3 Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse/rat Flt-3 Ligand.

Recovery

The recovery of mouse Flt-3 Ligand spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=5) 99 88-107
Mouse Serum (n=5) 95 82-106
Rat Cell Culture Supernates (n=5) 108 99-119
Rat Serum (n=5) 95 86-114

Linearity

To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse Flt-3 Ligand in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse/Rat Flt-3 Ligand ELISA Linearity

Scientific Data

Mouse/Rat Flt-3 Ligand ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Flt-3 Ligand/FLT3L

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Long Name:
fms-like Tyrosine Kinase 3 Ligand
Entrez Gene IDs:
2323 (Human); 14256 (Mouse); 102125470 (Cynomolgus Monkey); 493796 (Feline)
Alternate Names:
FL; FLG3L; Flt3 ligand; Flt-3 Ligand; Flt3L; FLT3LG; fms-related tyrosine kinase 3 ligand; SL cytokine
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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  1. PEGylated thrombopoietin mimetic, JNJ?26366821 a novel prophylactic radiation countermeasure for acute radiation injury
    Authors: Holmes-Hampton, GP;Kumar, VP;Biswas, S;Stone, S;Sharma, NK;Legesse, B;Vercellino, J;Guha, C;Eichenbaum, G;Ghosh, SP;
    Scientific reports
    Species: Mouse
    Sample Types: Serum
  2. Gut dysbiosis promotes the breakdown of oral tolerance mediated through dysfunction of mucosal dendritic cells
    Authors: T Fukaya, T Uto, S Mitoma, H Takagi, Y Nishikawa, M Tominaga, N Choijookhu, Y Hishikawa, K Sato
    Cell Reports, 2023-04-25;42(5):112431.
    Species: Mouse
    Sample Types: Serum
  3. Activation of neuronal FLT3 promotes exaggerated sensorial and emotional pain-related behaviors facilitating the transition from acute to chronic pain
    Authors: A Tassou, M Thouaye, D Gilabert, A Jouvenel, JP Leyris, C Sonrier, L Diouloufet, I Mechaly, S Mallié, J Bertin, M Chentouf, M Neiveyans, M Pugnière, P Martineau, B Robert, X Capdevila, J Valmier, C Rivat
    Progress in neurobiology, 2023-01-13;0(0):102405.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy
    Authors: J Zhu, Y Ke, Q Liu, J Yang, F Liu, R Xu, H Zhou, A Chen, J Xiao, F Meng, L Yu, R Li, J Wei, B Liu
    Nature Communications, 2022-12-03;13(1):7466.
    Species: Bacteria - Lactococcus lactis
    Sample Types: Cell Lysates
  5. Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality
    Authors: VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh
    Genes, 2022-09-28;13(10):.
    Species: Mouse
    Sample Types: Serum
  6. Mitigation of total body irradiation-induced mortality and hematopoietic injury of mice by a thrombopoietin mimetic (JNJ-26366821)
    Authors: VP Kumar, GP Holmes-Ham, S Biswas, S Stone, NK Sharma, B Hritzo, M Guilfoyle, G Eichenbaum, C Guha, SP Ghosh
    Scientific Reports, 2022-03-03;12(1):3485.
    Species: Mouse
    Sample Types: Serum
  7. Differences in Cell-Intrinsic Inflammatory Programs of Yolk Sac and Bone Marrow Macrophages
    Authors: S Elhag, C Stremmel, A Zehrer, J Plocke, R Hennel, M Keuper, C Knabe, J Winterhalt, V Gölling, L Tomas, T Weinberger, M Fischer, L Liu, F Wagner, M Lorenz, K Stark, H Häcker, M Schmidt-Su, U Völker, M Jastroch, K Lauber, T Straub, B Walzog, E Hammer, C Schulz
    Cells, 2021-12-17;10(12):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Gremlin 1+ fibroblastic niche maintains dendritic cell homeostasis in lymphoid tissues
    Authors: VN Kapoor, S Müller, S Keerthivas, M Brown, C Chalouni, EE Storm, A Castiglion, R Lane, M Nitschke, CX Dominguez, JL Astarita, AT Krishnamur, CB Carbone, Y Senbabaogl, AW Wang, X Wu, V Cremasco, M Roose-Girm, L Tam, J Doerr, MZ Chen, WP Lee, Z Modrusan, YA Yang, R Bourgon, W Sandoval, AS Shaw, FJ de Sauvage, I Mellman, C Moussion, SJ Turley
    Nature Immunology, 2021-04-26;22(5):571-585.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. microRNA and Metabolite Signatures Linked to Early Consequences of Lethal Radiation
    Authors: N Chakrabort, A Gautam, GP Holmes-Ham, VP Kumar, S Biswas, R Kumar, D Hamad, G Dimitrov, AO Olabisi, R Hammamieh, SP Ghosh
    Sci Rep, 2020-03-25;10(1):5424.
    Species: Mouse
    Sample Types: Serum
  10. Mesenchymal stem cell-mediated Notch2 activation overcomes radiation-induced injury of the hematopoietic system
    Authors: A Kim, S Shim, MJ Kim, JK Myung, S Park
    Sci Rep, 2018-06-18;8(1):9277.
    Species: Mouse
    Sample Types: Plasma
  11. Threshold levels of Flt3-ligand are required for the generation and survival of lymphoid progenitors and B-cell precursors.
    Authors: Dolence JJ, Gwin K, Frank E, Medina KL
    Eur. J. Immunol., 2011-02-01;41(2):324-34.
    Species: Mouse
    Sample Types: Serum
  12. Flt3 permits survival during infection by rendering dendritic cells competent to activate NK cells.
    Authors: Eidenschenk C, Crozat K, Krebs P, Arens R, Popkin D, Arnold CN, Blasius AL, Benedict CA, Moresco EM, Xia Y, Beutler B
    Proc. Natl. Acad. Sci. U.S.A., 2010-05-10;107(21):9759-64.
    Species: Mouse
    Sample Types: Serum
  13. Homeostasis of dendritic cells in lymphoid organs is controlled by regulation of their precursors via a feedback loop.
    Authors: Hochweller K, Miloud T, Striegler J, Naik S, Hammerling GJ, Garbi N
    Blood, 2009-09-18;114(20):4411-21.
    Species: Mouse
    Sample Types: Serum
  14. Ectodomain shedding of FLT3 ligand is mediated by TNF-alpha converting enzyme.
    Authors: Horiuchi K, Morioka H, Takaishi H, Akiyama H, Blobel CP, Toyama Y
    J. Immunol., 2009-06-15;182(12):7408-14.
    Species: Mouse
    Sample Types: Serum
  15. Knock-in of an internal tandem duplication mutation into murine FLT3 confers myeloproliferative disease in a mouse model.
    Authors: Li L, Piloto O, Nguyen HB, Greenberg K, Takamiya K, Racke F, Huso D, Small D
    Blood, 2008-02-01;111(7):3849-58.
    Species: Mouse
    Sample Types: Serum
  16. Effects of DNA- and Mycobacterium bovis BCG-based delivery of the Flt3 ligand on protective immunity to Mycobacterium tuberculosis.
    Authors: Triccas JA, Shklovskaya E, Spratt J, Ryan AA, Palendira U, Fazekas de St Groth B, Britton WJ
    Infect. Immun., 2007-08-27;75(11):5368-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. Nanosized bioceramic particles could function as efficient gene delivery vehicles with target specificity for the spleen.
    Authors: Tan K, Cheang P, Ho IA, Lam PY, Hui KM
    Gene Ther., 2007-03-08;14(10):828-35.
    Species: Mouse
    Sample Types: Serum
  18. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007-02-01;178(3):1553-63.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. The long-term but not the short-term antiviral effect of IFN-alpha depends on Flt3 ligand and pDC.
    Authors: Vollstedt S, O&amp;apos;Keeffe M, Ryf B, Glanzmann B, Hochrein H, Suter M
    Eur. J. Immunol., 2006-05-01;36(5):1231-40.
    Species: Mouse
    Sample Types: Serum
  20. Fms-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the brain.
    Authors: Curtin JF, King GD, Barcia C, Liu C, Hubert FX, Guillonneau C, Josien R, Anegon I, Lowenstein PR, Castro MG
    J. Immunol., 2006-03-15;176(6):3566-77.
    Species: Rat
    Sample Types: Serum
  21. Inflammatory and anti-glioma effects of an adenovirus expressing human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment with hsFlt3L inhibits intracranial glioma progression.
    Authors: Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, Puntel M, Goverdhana S, Lowenstein PR, Castro MG
    Mol. Ther., 2004-12-01;10(6):1071-84.
    Species: Rat
    Sample Types: Tissue Homogenates
  22. Conditional expression of murine Flt3 ligand leads to expansion of multiple dendritic cell subsets in peripheral blood and tissues of transgenic mice.
    Authors: Manfra DJ, Chen SC, Jensen KK, Fine JS, Wiekowski MT, Lira SA
    J. Immunol., 2003-03-15;170(6):2843-52.
    Species: Mouse
    Sample Types: Serum
  23. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
    Authors: Miller G, 107331, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
    Lamin A/C impairments cause mitochondrial dysfunction by attenuating PGC1alpha and the NAMPT-NAD+ pathway, 2002-09-15;169(6):2875-85.
    Species: Mouse
    Sample Types: Serum
  24. Modulation of marrow proliferation and chemosensitivity by tumor-produced cytokines from syngeneic pancreatic tumor lines.
    Authors: Blumenthal RD, Reising A, Leon E
    Clin. Cancer Res., 2002-05-01;8(5):1301-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Mouse/Rat Flt-3 Ligand/FLT3L Quantikine ELISA Kit
By Anonymous on 10/26/2022
Sample Tested: Cell culture supernatant

Cell culture supernatants from BHK-21 cells