HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
Mouse/Rat HGF Quantikine ELISA Kit
R&D Systems | Catalog # MHG00
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (25 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL)
Sensitivity
12.1 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)
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Product Summary for Mouse/Rat HGF Quantikine ELISA Kit
The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse, Rat
Specificity
Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Platelet-poor EDTA Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 217 | 452 | 1168 | 218 | 447 | 1107 |
| Standard Deviation | 8.6 | 19.7 | 62.2 | 14.9 | 21.0 | 87.7 |
| CV% | 4.0 | 4.4 | 5.3 | 6.8 | 4.7 | 7.9 |
Recovery for Mouse/Rat HGF Quantikine ELISA Kit
The recovery of HGF spiked into various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=4) | 98 | 85-106 |
| Mouse Platelet-poor EDTA Plasma (n=4) | 104 | 101-106 |
| Mouse Serum (n=4) | 96 | 84-112 |
| Mouse Tissue Homogenates (n=3) | 87 | 80-101 |
| Rat Cell Culture Supernates (n=4) | 102 | 85-114 |
| Rat Platelet-poor EDTA Plasma (n=4) | 108 | 100-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat HGF Quantikine ELISA Kit
Mouse/Rat HGF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HGF
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
Additional HGF Products
Product Documents for Mouse/Rat HGF Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat HGF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse/Rat HGF Quantikine ELISA Kit
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Sample Tested: Serum and PlasmaVerified Customer | Posted 02/25/2023
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Sample Tested: Cell culture supernatantVerified Customer | Posted 06/28/2022Calibration curve performed greatly.
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Protocols
View specific protocols for Mouse/Rat HGF Quantikine ELISA Kit (MHG00):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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