Mouse/Rat HGF Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
MHG00
HGF ELISA Kit ELISA
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Procedure
Citations (12)
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Mouse/Rat HGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)
Sensitivity
12.1 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)
Specificity
Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 217 452 1168 218 447 1107
Standard Deviation 8.6 19.7 62.2 14.9 21 87.7
CV% 4 4.4 5.3 6.8 4.7 7.9

Recovery

The recovery of HGF spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 98 85-106
Mouse Platelet-poor EDTA Plasma (n=4) 104 101-106
Mouse Serum (n=4) 96 84-112
Mouse Tissue Homogenates (n=3) 87 80-101
Rat Cell Culture Supernates (n=4) 102 85-114
Rat Platelet-poor EDTA Plasma (n=4) 108 100-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat HGF ELISA Linearity
Mouse/Rat HGF ELISA Linearity

Data Examples

Mouse/Rat HGF ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Long Name:
Hepatocyte Growth Factor
Entrez Gene IDs:
3082 (Human); 15234 (Mouse); 403441 (Canine)
Alternate Names:
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat HGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells
    Authors: X Chen, Y Zhang, W Wang, Z Liu, J Meng, Z Han
    Cell. Physiol. Biochem., 2018;49(1):101-122.
    Species: Rat
    Sample Types: Cell Culture Supernates
  2. Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo
    Authors: ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon
    Int J Mol Sci, 2018;19(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Cardiac cell-integrated microneedle patch for treating myocardial infarction
    Authors: J Tang, J Wang, K Huang, Y Ye, T Su, L Qiao, MT Hensley, TG Caranasos, J Zhang, Z Gu, K Cheng
    Sci Adv, 2018;4(11):eaat9365.
    Species: Rat
    Sample Types: Cell Culture Supernates
  4. Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance through ER?/HGF/MET Pathway
    Authors: YY Zhang, C Li, GF Yao, LJ Du, Y Liu, XJ Zheng, S Yan, JY Sun, Y Liu, MZ Liu, X Zhang, G Wei, W Tong, X Chen, Y Wu, S Sun, S Liu, Q Ding, Y Yu, H Yin, SZ Duan
    Diabetes, 2017;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  6. First steps to define murine amniotic fluid stem cell microenvironment
    Sci Rep, 2016;6(0):37080.
    Species: Mouse
    Sample Types: Amniotic Fluid
  7. BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality
    Sci Rep, 2016;6(0):32399.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Microvesicles derived from human umbilical cord mesenchymal stem cells facilitate tubular epithelial cell dedifferentiation and growth via hepatocyte growth factor induction.
    Authors: Ju, Guan-qun, Cheng, Jun, Zhong, Liang, Wu, Shuai, Zou, Xiang-yu, Zhang, Guang-yu, Gu, Di, Miao, Shuai, Zhu, Ying-jia, Sun, Jie, Du, Tao
    PLoS ONE, 2015;10(3):e0121534.
    Species: Rat
    Sample Types: Cell Culture Supernates
  9. The anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2.
    Sci Rep, 2015;5(0):8713.
    Species: Rat
    Sample Types: Serum
  10. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
    Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
    PLoS ONE, 2015;10(5):e0125797.
  11. Pharmacological Inhibition of KIT Activates MET Signaling in Gastrointestinal Stromal Tumors.
    Authors: Cohen N, Zeng S, Seifert A, Kim T, Sorenson E, Greer J, Beckman M, Santamaria-Barria J, Crawley M, Green B, Rossi F, Besmer P, Antonescu C, DeMatteo R
    Cancer Res, 2015;75(10):2061-70.
    Species: Mouse
    Sample Types: Serum
  12. Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds.
    Authors: Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G
    Stem Cells Transl Med, 2014;3(9):1079-89.
    Species: Mouse
    Sample Types: Protein

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