Mouse/Rat HGF Quantikine ELISA Kit

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MHG00
Mouse/Rat HGF ELISA Standard Curve
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Product Details
Procedure
Citations (29)
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Reviews (2)

Mouse/Rat HGF Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)
Sensitivity
12.1 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)
Specificity
Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Recovery

The recovery of HGF spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 98 85-106
Mouse Platelet-poor EDTA Plasma (n=4) 104 101-106
Mouse Serum (n=4) 96 84-112
Mouse Tissue Homogenates (n=3) 87 80-101
Rat Cell Culture Supernates (n=4) 102 85-114
Rat Platelet-poor EDTA Plasma (n=4) 108 100-116

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat HGF ELISA Linearity
Mouse/Rat HGF ELISA Linearity

Scientific Data

Mouse/Rat HGF ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HGF

HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.

Long Name:
Hepatocyte Growth Factor
Entrez Gene IDs:
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names:
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat HGF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Ideal Duration of Pretreatment Using a Gelatin Hydrogel Nonwoven Fabric Prior to Subcutaneous Islet Transplantation
    Authors: Saito, R;Inagaki, A;Nakamura, Y;Imura, T;Kanai, N;Mitsugashira, H;Endo, Y;Katano, T;Suzuki, S;Tokodai, K;Kamei, T;Unno, M;Watanabe, K;Tabata, Y;Goto, M;
    Cell transplantation
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. The acid-sensing nociceptor TRPV1 controls breast cancer progression in bone via regulating HGF secretion from sensory neurons
    Authors: Okui, T;Hiasa, M;Hata, K;Roodman, GD;Nakanishi, M;Yoneda, T;
    Research square
    Species: Mouse
    Sample Types: Bone Marrow, Serum
  3. Combinational Treatment Involving Decellularized Extracellular Matrix Hydrogels With Mesenchymal Stem Cells Increased the Efficacy of Cell Therapy in Pancreatitis
    Authors: Kojima, H;Kushige, H;Yagi, H;Nishijima, T;Moritoki, N;Nagoshi, N;Nakano, Y;Tanaka, M;Hori, S;Hasegawa, Y;Abe, Y;Kitago, M;Nakamura, M;Kitagawa, Y;
    Cell transplantation
    Species: Rat
    Sample Types: Cell Culture Supernates
  4. Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin
    Authors: Y Iso, S Usui, H Suzuki
    Pharmaceutics, 2023-03-24;15(4):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  5. Impact of endothelial nitric oxide synthase activation on accelerated liver regeneration in a rat ALPPS model
    Authors: H Masuo, A Shimizu, H Motoyama, K Kubota, T Notake, T Yoshizawa, K Hosoda, K Yasukawa, A Kobayashi, Y Soejima
    World Journal of Gastroenterology, 2023-02-07;29(5):867-878.
    Species: Rat
    Sample Types: Serum
  6. Effect of pretreatment with a synbiotic on Perfluorooctanoic acid-induced liver damage after sub-acute oral exposure in C57BL/6J mice
    Authors: M Soltani, M Pourfarzam, AH Sharifabad, AK Neisiani, MK Mousavi, M Aliomrani
    Toxicology and Applied Pharmacology, 2022-12-23;459(0):116360.
    Species: Mouse
    Sample Types: Serum
  7. Therapeutic effects of adipose-derived mesenchymal stem/stromal cells with enhanced migration ability and hepatocyte growth factor secretion by low-molecular-weight heparin treatment in bleomycin-induced mouse models of systemic sclerosis
    Authors: T Suzuka, T Kotani, T Saito, S Matsuda, T Sato, T Takeuchi
    Arthritis Research & Therapy, 2022-10-07;24(1):228.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Dry preserved multilayered fibroblast cell sheets are a new manageable tool for regenerative medicine to promote wound healing
    Authors: Y Matsuno, M Yanagihara, K Ueno, T Saito, H Kurazumi, R Suzuki, S Katsura, A Oga, K Hamano
    Scientific Reports, 2022-07-22;12(1):12519.
    Species: Mouse
    Sample Types: Cell Lysates
  9. Combination Therapy of Placenta-Derived Mesenchymal Stem Cells with WKYMVm Promotes Hepatic Function in a Rat Model with Hepatic Disease via Vascular Remodeling
    Authors: JH Jun, S Park, JY Kim, JY Lim, GT Park, JH Kim, GJ Kim
    Cells, 2022-01-11;11(2):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  10. Enhanced cellular engraftment of adipose-derived mesenchymal stem cell spheroids by using nanosheets as scaffolds
    Authors: H Nagano, Y Suematsu, M Takuma, S Aoki, A Satoh, E Takayama, M Kinoshita, Y Morimoto, S Takeoka, T Fujie, T Kiyosawa
    Scientific Reports, 2021-07-14;11(1):14500.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Analysis of structural components of decellularized scaffolds in renal fibrosis
    Authors: R Zhang, J Jiang, Y Yu, F Wang, N Gao, Y Zhou, X Wan, Z Wang, P Wei, J Mei
    Bioactive materials, 2021-01-16;6(7):2187-2197.
    Species: Rat
    Sample Types: Cell Culture Supernates
  12. Antifibrotic therapy by sustained release of low molecular weight heparin from poly(lactic-co-glycolic acid) microparticles on bleomycin-induced pulmonary fibrosis in mice
    Authors: T Saito, T Kotani, K Suzuki
    Sci Rep, 2020-11-04;10(1):19019.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns
    Authors: GW Park, J Heo, JY Kang, JW Yang, JS Kim, KD Kwon, BC Yu, SJ Lee
    Sci Rep, 2020-07-24;10(1):12448.
    Species: Rat
    Sample Types: Cell Culture Supernates
  14. The neovascularization effect of dedifferentiated fat cells
    Authors: H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto
    Sci Rep, 2020-06-08;10(1):9211.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Impaired integrin &alpha5 /&beta1 -mediated hepatocyte growth factor release by stellate cells of the aged liver
    Authors: F Rohn, C Kordes, T Buschmann, D Reichert, M Wammers, G Poschmann, K Stühler, AS Benk, F Geiger, JP Spatz, D Häussinger
    Aging Cell, 2020-03-11;0(0):e13131.
    Species: Rat
    Sample Types: Cell Culture Supernates
  16. Impact of obesity on breast cancer recurrence and minimal residual disease
    Authors: BL Ecker, JY Lee, CJ Sterner, AC Solomon, DK Pant, F Shen, J Peraza, L Vaught, S Mahendra, GK Belka, TC Pan, KH Schmitz, LA Chodosh
    Breast Cancer Res., 2019-03-13;21(1):41.
    Species: Mouse
    Sample Types: Serum
  17. Cardiac cell-integrated microneedle patch for treating myocardial infarction
    Authors: J Tang, J Wang, K Huang, Y Ye, T Su, L Qiao, MT Hensley, TG Caranasos, J Zhang, Z Gu, K Cheng
    Sci Adv, 2018-11-28;4(11):eaat9365.
    Species: Rat
    Sample Types: Cell Culture Supernates
  18. Mesenchymal Stem Cells Modified with Heme Oxygenase-1 Have Enhanced Paracrine Function and Attenuate Lipopolysaccharide-Induced Inflammatory and Oxidative Damage in Pulmonary Microvascular Endothelial Cells
    Authors: X Chen, Y Zhang, W Wang, Z Liu, J Meng, Z Han
    Cell. Physiol. Biochem., 2018-08-28;49(1):101-122.
    Species: Rat
    Sample Types: Cell Culture Supernates
  19. Decellularized Diaphragmatic Muscle Drives a Constructive Angiogenic Response In Vivo
    Authors: ME Alvarèz Fa, M Piccoli, C Franzin, A Sgrò, A Dedja, L Urbani, E Bertin, C Trevisan, P Gamba, AJ Burns, P De Coppi, M Pozzobon
    Int J Mol Sci, 2018-04-28;19(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Radiofrequency ablation (RFA) induced systemic tumor growth can be reduced by suppression of resultant heat shock proteins
    Authors: M Ahmed, G Kumar, S Gourevitch, T Levchenko, E Galun, V Torchilin, SN Goldberg
    Int J Hyperthermia, 2018-04-24;0(0):1-25.
    Species: Rat
    Sample Types: Serum
  21. Deletion of Macrophage Mineralocorticoid Receptor Protects Hepatic Steatosis and Insulin Resistance through ER?/HGF/MET Pathway
    Authors: YY Zhang, C Li, GF Yao, LJ Du, Y Liu, XJ Zheng, S Yan, JY Sun, Y Liu, MZ Liu, X Zhang, G Wei, W Tong, X Chen, Y Wu, S Sun, S Liu, Q Ding, Y Yu, H Yin, SZ Duan
    Diabetes, 2017-03-21;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016-12-19;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. First steps to define murine amniotic fluid stem cell microenvironment
    Sci Rep, 2016-11-15;6(0):37080.
    Species: Mouse
    Sample Types: Amniotic Fluid
  24. BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality
    Sci Rep, 2016-08-26;6(0):32399.
    Species: Mouse
    Sample Types: Tissue Homogenates
  25. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
    Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
    PLoS ONE, 2015-05-04;10(5):e0125797.
    Species: Mouse
    Sample Types: BALF
  26. Pharmacological Inhibition of KIT Activates MET Signaling in Gastrointestinal Stromal Tumors.
    Authors: Cohen N, Zeng S, Seifert A, Kim T, Sorenson E, Greer J, Beckman M, Santamaria-Barria J, Crawley M, Green B, Rossi F, Besmer P, Antonescu C, DeMatteo R
    Cancer Res, 2015-04-02;75(10):2061-70.
    Species: Mouse
    Sample Types: Serum
  27. Microvesicles derived from human umbilical cord mesenchymal stem cells facilitate tubular epithelial cell dedifferentiation and growth via hepatocyte growth factor induction.
    Authors: Ju, Guan-qun, Cheng, Jun, Zhong, Liang, Wu, Shuai, Zou, Xiang-yu, Zhang, Guang-yu, Gu, Di, Miao, Shuai, Zhu, Ying-jia, Sun, Jie, Du, Tao
    PLoS ONE, 2015-03-20;10(3):e0121534.
    Species: Rat
    Sample Types: Cell Culture Supernates
  28. The anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2.
    Sci Rep, 2015-03-04;5(0):8713.
    Species: Rat
    Sample Types: Serum
  29. Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds.
    Authors: Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G
    Stem Cells Transl Med, 2014-07-18;3(9):1079-89.
    Species: Mouse
    Sample Types: Protein

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Reviews for Mouse/Rat HGF Quantikine ELISA Kit

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Mouse/Rat HGF Quantikine ELISA Kit
By Anonymous on 02/25/2023
Sample Tested: Serum and Plasma

Mouse/Rat HGF Quantikine ELISA Kit
By Anonymous on 06/28/2022
Sample Tested: Cell culture supernatant

Calibration curve performed greatly.