• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (25 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL)
  • Sensitivity
    12.1 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma)
  • Specificity
    Natural and recombinant HGF. Recombinant mouse HGF single chain precursor cross-reacts 100% in this assay.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat HGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse or rat HGF in cell culture supernates, tissue homogenates, serum, and platelet-poor plasma. It contains NS0-expressed recombinant mouse HGF and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural HGF showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural HGF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean21745211682184471107
Standard Deviation8.619.762.214.92187.7
CV%44.45.36.84.77.9

Recovery

The recovery of HGF spiked into various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 98 85-106
Mouse Platelet-poor EDTA Plasma (n=4) 104 101-106
Mouse Serum (n=4) 96 84-112
Mouse Tissue Homogenates (n=3) 87 80-101
Rat Cell Culture Supernates (n=4) 102 85-114
Rat Platelet-poor EDTA Plasma (n=4) 108 100-116
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse/rat HGF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat HGF Quantikine ELISA Kit
Mouse/Rat HGF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HGF
HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
    • Long Name
      Hepatocyte Growth Factor
    • Entrez Gene IDs
      3082 (Human); 15234 (Mouse); 403441 (Canine);
    • Alternate Names
      deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    8 Citations: Showing 1 - 8
    Filter your results:

    Species
    Sample Type
    1. First steps to define murine amniotic fluid stem cell microenvironment
      Sci Rep, 2016;6(0):37080.
      Species: Mouse
      Sample Type: Amniotic Fluid
    2. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
      Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
      Species: Rat
      Sample Type: Tissue Homogenates
    3. BubR1 Insufficiency Impairs Liver Regeneration in Aged Mice after Hepatectomy through Intercalated Disc Abnormality
      Sci Rep, 2016;6(0):32399.
      Species: Mouse
      Sample Type: Tissue Homogenates
    4. Microvesicles derived from human umbilical cord mesenchymal stem cells facilitate tubular epithelial cell dedifferentiation and growth via hepatocyte growth factor induction.
      Authors: Ju, Guan-qun, Cheng, Jun, Zhong, Liang, Wu, Shuai, Zou, Xiang-yu, Zhang, Guang-yu, Gu, Di, Miao, Shuai, Zhu, Ying-jia, Sun, Jie, Du, Tao
      PLoS ONE, 2015;10(3):e0121534.
      Species: Rat
      Sample Type: Cell Culture Supernates
    5. The anti-fibrotic effects of mesenchymal stem cells on irradiated lungs via stimulating endogenous secretion of HGF and PGE2.
      Sci Rep, 2015;5(0):8713.
      Species: Rat
      Sample Type: Serum
    6. Regulation of hepatocyte growth factor in mice with pneumonia by peptidases and trans-alveolar flux.
      Authors: Raymond W, Xu X, Nimishakavi S, Le C, McDonald D, Caughey G
      PLoS ONE, 2015;10(5):e0125797.
    7. Pharmacological Inhibition of KIT Activates MET Signaling in Gastrointestinal Stromal Tumors.
      Authors: Cohen N, Zeng S, Seifert A, Kim T, Sorenson E, Greer J, Beckman M, Santamaria-Barria J, Crawley M, Green B, Rossi F, Besmer P, Antonescu C, DeMatteo R
      Cancer Res, 2015;75(10):2061-70.
      Species: Mouse
      Sample Type: Serum
    8. Capillary force seeding of hydrogels for adipose-derived stem cell delivery in wounds.
      Authors: Garg R, Rennert R, Duscher D, Sorkin M, Kosaraju R, Auerbach L, Lennon J, Chung M, Paik K, Nimpf J, Rajadas J, Longaker M, Gurtner G
      Stem Cells Transl Med, 2014;3(9):1079-89.
      Species: Mouse
      Sample Type: Protein
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Cell Lysis Buffer 2

    ELISA 895347 2
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....

    Have you used Mouse/Rat HGF Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Order Details

    Contact Information

    R&D Systems Guarantee

    Customers Who Viewed
    This Item Also Viewed

    Feedback

    Customer Information