Mouse/Rat IL-22 Quantikine ELISA Kit

  (26 citations)     
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Assay Procedure
Citations (26)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    8.2 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse and rat IL-22
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse/Rat IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-22 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-22 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-22.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 35 35 35
Mean 45.9 129 628 43.9 122 596
Standard Deviation 1.9 7.5 22.1 3.4 6.7 25.2
CV% 4.2 5.8 3.5 7.7 5.5 4.2

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 34 34 34
Mean 44.4 117 692 45.4 128 662
Standard Deviation 2 7 25.7 4.8 7.5 35.5
CV% 4.4 6 3.7 10.5 5.9 5.4

Recovery

The recovery of IL-22 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 98 93-103
Mouse EDTA Plasma (n=4) 95 86-106
Mouse Heparin Plasma (n=4) 94 83-102
Mouse Serum (n=4) 91 84-99
Rat Cell Culture Supernates (n=4) 98 91-102
Rat EDTA Plasma (n=4) 97 91-111
Rat Heparin Plasma (n=4) 93 88-101
Rat Serum (n=4) 96 90-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-22 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
 IL-22 [HRP]
 IL-22 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
  • Long Name:
    Interleukin 22
  • Entrez Gene IDs:
    50616 (Human); 50929 (Mouse); 500836 (Rat)
  • Alternate Names:
    Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 120 µL Substrate Solution
  12.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 120 µL Stop Solution
  14.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Sample Type
  1. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
  2. Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer's patches
    Authors: SH Kim, BH Cho, H Kiyono, YS Jang
    Sci Rep, 2017;7(1):3980.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  3. Ablation of IL-17 expression moderates experimental autoimmune myasthenia gravis disease severity
    Authors: G Aguilo-Sea, Y Xie, J Sheehan, LL Kusner, HJ Kaminski
    Cytokine, 2017;96(0):279-285.
    Species: Mouse
    Sample Type: Serum
  4. Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro
    Authors: WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song
    Mol Med Rep, 2017;15(5):2551-2559.
    Species: Rat
    Sample Type: Cell Culture Supernates
  5. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Mouse
    Sample Type: BALF
  6. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
    Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
    Nat Immunol, 2015;16(3):286-95.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  7. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
    Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
    Infect Immun, 2014;82(9):3723-39.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
    Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
    J Immunol, 2014;193(5):2330-9.
  9. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis.
    Authors: Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y
    Mol Med Rep, 2014;9(6):2097-104.
    Species: Mouse
    Sample Type: Serum
  10. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
    Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
    PLoS Pathog., 2012;8(7):e1002799.
    Species: Mouse
    Sample Type: Serum
  11. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012;7(9):e46311.
    Species: Mouse
    Sample Type: Vaginal Lavage
  12. IL-22 signaling contributes to West Nile encephalitis pathogenesis.
    Authors: Wang, Penghua, Bai, Fengwei, Zenewicz, Lauren A, Dai, Jianfeng, Gate, David, Cheng, Gong, Yang, Long, Qian, Feng, Yuan, Xiaoling, Montgomery, Ruth R, Flavell, Richard, Town, Terrence, Fikrig, Erol
    PLoS ONE, 2012;7(8):e44153.
    Species: Mouse
    Sample Type: Plasma
  13. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
    Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
    Infect. Immun., 2012;80(1):410-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  14. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
    Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
    J. Exp. Med., 2011;208(3):491-503.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  15. A novel role for IL-22R1 as a driver of inflammation.
    Authors: Savan R, McFarland AP, Reynolds DA
    Blood, 2011;117(2):575-84.
    Species: Mouse
    Sample Type: Serum
  16. IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6.
    Authors: Lindroos J, Svensson L, Norsgaard H
    J. Invest. Dermatol., 2011;131(5):1110-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
  17. Influenza A inhibits Th17-mediated host defense against bacterial pneumonia in mice.
    Authors: Kudva A, Scheller EV, Robinson KM
    J. Immunol., 2011;186(3):1666-74.
    Species: Mouse
    Sample Type: BALF
  18. IL-23 and IL-17A, but not IL-12 and IL-22, are required for optimal skin host defense against Candida albicans.
    Authors: Kagami S, Rizzo HL, Kurtz SE
    J. Immunol., 2010;185(9):5453-62.
    Species: Mouse
    Sample Type: Tissue Homogenates
  19. Phosphodiesterase 7A inhibitor ASB16165 suppresses proliferation and cytokine production of NKT cells.
    Authors: Goto M, Murakawa M, Kadoshima-Yamaoka K, Tanaka Y, Inoue H, Murafuji H, Hayashi Y, Miura K, Nakatsuka T, Nagahira K, Chamoto K, Fukuda Y, Nishimura T
    Cell. Immunol., 2009;258(2):147-51.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  20. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
    Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
    Int. Immunol., 2009;21(5):555-65.
    Species: Mouse
    Sample Type: Serum
  21. Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo.
    Authors: Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H
    Clin. Exp. Immunol., 2009;157(3):370-6.
    Species: Mouse
    Sample Type: Plasma
  22. IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency.
    Authors: Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J
    J. Immunol., 2009;182(8):4521-8.
    Species: Mouse
    Sample Type: Serum
  23. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
    Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
    Nat. Med., 2008;14(3):275-81.
    Species: Mouse
    Sample Type: Tissue Homogenates
  24. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
    Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
    J. Immunol., 2008;181(6):4089-97.
    Species: Mouse
    Sample Type: BALF
  25. TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology.
    Authors: McGeachy MJ, Bak-Jensen KS, Chen Y, Tato CM, Blumenschein W, McClanahan T, Cua DJ
    Nat. Immunol., 2007;8(12):1390-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  26. The development of inflammatory T(H)-17 cells requires interferon-regulatory factor 4.
    Authors: Brustle A, Heink S, Huber M, Rosenplanter C, Stadelmann C, Yu P, Arpaia E, Mak TW, Kamradt T, Lohoff M
    Nat. Immunol., 2007;8(9):958-66.
    Species: Mouse
    Sample Type: Cell Culture Supernates
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