• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
  • Sensitivity
    8.2 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse and rat IL-22
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse/Rat IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-22 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-22 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-22.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020343434
Mean44.411769245.4128662
Standard Deviation2725.74.87.535.5
CV%4.463.710.55.95.4

Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020353535
Mean45.912962843.9122596
Standard Deviation1.97.522.13.46.725.2
CV%4.25.83.57.75.54.2

Recovery

The recovery of IL-22 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=4) 98 93-103
Mouse EDTA Plasma (n=4) 95 86-106
Mouse Heparin Plasma (n=4) 94 83-102
Mouse Serum (n=4) 91 84-99
Rat Cell Culture Supernates (n=4) 98 91-102
Rat EDTA Plasma (n=4) 97 91-111
Rat Heparin Plasma (n=4) 93 88-101
Rat Serum (n=4) 96 90-107
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-22 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
Mouse/Rat IL-22 Quantikine ELISA Kit
Mouse/Rat IL-22 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-22
IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
    • Long Name
      Interleukin 22
    • Entrez Gene IDs
      50616 (Human); 50929 (Mouse); 500836 (Rat);
    • Alternate Names
      Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 120 µL Substrate Solution
    12.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 120 µL Stop Solution
    14.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    26 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
      Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
      J. Immunol., 2017;0(0):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer's patches
      Authors: SH Kim, BH Cho, H Kiyono, YS Jang
      Sci Rep, 2017;7(1):3980.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    3. Ablation of IL-17 expression moderates experimental autoimmune myasthenia gravis disease severity
      Authors: G Aguilo-Sea, Y Xie, J Sheehan, LL Kusner, HJ Kaminski
      Cytokine, 2017;96(0):279-285.
      Species: Mouse
      Sample Type: Serum
    4. Biological effects of bone marrow mesenchymal stem cells on hepatitis B virus in�vitro
      Authors: WP Zheng, BY Zhang, ZY Shen, ML Yin, Y Cao, HL Song
      Mol Med Rep, 2017;15(5):2551-2559.
      Species: Rat
      Sample Type: Cell Culture Supernates
    5. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
      Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
      J Microbiol Immunol Infect, 2017;0(0):.
      Species: Mouse
      Sample Type: BALF
    6. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance.
      Authors: Basu R, Whitley S, Bhaumik S, Zindl C, Schoeb T, Benveniste E, Pear W, Hatton R, Weaver C
      Nat Immunol, 2015;16(3):286-95.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner.
      Authors: Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R
      Infect Immun, 2014;82(9):3723-39.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
      Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
      J Immunol, 2014;193(5):2330-9.
    9. Endogenous n-3 polyunsaturated fatty acids protect against imiquimod-induced psoriasis-like inflammation via the IL-17/IL-23 axis.
      Authors: Qin S, Wen J, Bai X, Chen T, Zheng R, Zhou G, Ma J, Feng J, Zhong B, Li Y
      Mol Med Rep, 2014;9(6):2097-104.
      Species: Mouse
      Sample Type: Serum
    10. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
      Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
      PLoS Pathog., 2012;8(7):e1002799.
      Species: Mouse
      Sample Type: Serum
    11. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
      Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
      PLoS ONE, 2012;7(9):e46311.
      Species: Mouse
      Sample Type: Vaginal Lavage
    12. IL-22 signaling contributes to West Nile encephalitis pathogenesis.
      Authors: Wang, Penghua, Bai, Fengwei, Zenewicz, Lauren A, Dai, Jianfeng, Gate, David, Cheng, Gong, Yang, Long, Qian, Feng, Yuan, Xiaoling, Montgomery, Ruth R, Flavell, Richard, Town, Terrence, Fikrig, Erol
      PLoS ONE, 2012;7(8):e44153.
      Species: Mouse
      Sample Type: Plasma
    13. Dectin-1-dependent interleukin-22 contributes to early innate lung defense against Aspergillus fumigatus.
      Authors: Gessner MA, Werner JL, Lilly LM, Nelson MP, Metz AE, Dunaway CW, Chan YR, Ouyang W, Brown GD, Weaver CT, Steele C
      Infect. Immun., 2012;80(1):410-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy.
      Authors: Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L
      J. Exp. Med., 2011;208(3):491-503.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    15. A novel role for IL-22R1 as a driver of inflammation.
      Authors: Savan R, McFarland AP, Reynolds DA
      Blood, 2011;117(2):575-84.
      Species: Mouse
      Sample Type: Serum
    16. IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6.
      Authors: Lindroos J, Svensson L, Norsgaard H
      J. Invest. Dermatol., 2011;131(5):1110-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    17. Influenza A inhibits Th17-mediated host defense against bacterial pneumonia in mice.
      Authors: Kudva A, Scheller EV, Robinson KM
      J. Immunol., 2011;186(3):1666-74.
      Species: Mouse
      Sample Type: BALF
    18. IL-23 and IL-17A, but not IL-12 and IL-22, are required for optimal skin host defense against Candida albicans.
      Authors: Kagami S, Rizzo HL, Kurtz SE
      J. Immunol., 2010;185(9):5453-62.
      Species: Mouse
      Sample Type: Tissue Homogenates
    19. Phosphodiesterase 7A inhibitor ASB16165 suppresses proliferation and cytokine production of NKT cells.
      Authors: Goto M, Murakawa M, Kadoshima-Yamaoka K, Tanaka Y, Inoue H, Murafuji H, Hayashi Y, Miura K, Nakatsuka T, Nagahira K, Chamoto K, Fukuda Y, Nishimura T
      Cell. Immunol., 2009;258(2):147-51.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    20. Differential IL-23 requirement for IL-22 and IL-17A production during innate immunity against Salmonella enterica serovar Enteritidis.
      Authors: Siegemund S, Schutze N, Schulz S, Wolk K, Nasilowska K, Straubinger RK, Sabat R, Alber G
      Int. Immunol., 2009;21(5):555-65.
      Species: Mouse
      Sample Type: Serum
    21. Dexamethasone suppresses interleukin-22 associated with bacterial infection in vitro and in vivo.
      Authors: Ziesche E, Scheiermann P, Bachmann M, Sadik CD, Hofstetter C, Zwissler B, Pfeilschifter J, Muhl H
      Clin. Exp. Immunol., 2009;157(3):370-6.
      Species: Mouse
      Sample Type: Plasma
    22. IL-22-dependent attenuation of T cell-dependent (ConA) hepatitis in herpes virus entry mediator deficiency.
      Authors: Wahl C, Wegenka UM, Leithauser F, Schirmbeck R, Reimann J
      J. Immunol., 2009;182(8):4521-8.
      Species: Mouse
      Sample Type: Serum
    23. IL-22 mediates mucosal host defense against Gram-negative bacterial pneumonia.
      Authors: Aujla SJ, Chan YR, Zheng M, Fei M, Askew DJ, Pociask DA, Reinhart TA, McAllister F, Edeal J, Gaus K, Husain S, Kreindler JL, Dubin PJ, Pilewski JM, Myerburg MM, Mason CA, Iwakura Y, Kolls JK
      Nat. Med., 2008;14(3):275-81.
      Species: Mouse
      Sample Type: Tissue Homogenates
    24. TH17 cells mediate steroid-resistant airway inflammation and airway hyperresponsiveness in mice.
      Authors: McKinley L, Alcorn JF, Peterson A, Dupont RB, Kapadia S, Logar A, Henry A, Irvin CG, Piganelli JD, Ray A, Kolls JK
      J. Immunol., 2008;181(6):4089-97.
      Species: Mouse
      Sample Type: BALF
    25. TGF-beta and IL-6 drive the production of IL-17 and IL-10 by T cells and restrain T(H)-17 cell-mediated pathology.
      Authors: McGeachy MJ, Bak-Jensen KS, Chen Y, Tato CM, Blumenschein W, McClanahan T, Cua DJ
      Nat. Immunol., 2007;8(12):1390-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    26. The development of inflammatory T(H)-17 cells requires interferon-regulatory factor 4.
      Authors: Brustle A, Heink S, Huber M, Rosenplanter C, Stadelmann C, Yu P, Arpaia E, Mak TW, Kamradt T, Lohoff M
      Nat. Immunol., 2007;8(9):958-66.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    Expand to show all 26 Citations
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