IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.
Mouse/Rat IL-22 Quantikine ELISA Kit
R&D Systems | Catalog # M2200
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
8.2 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
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Product Summary for Mouse/Rat IL-22 Quantikine ELISA Kit
The Quantikine Mouse/Rat IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-22 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-22 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-22.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse, Rat
Specificity
Natural and recombinant mouse and rat IL-22
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean (pg/mL) | 45.9 | 129 | 628 | 43.9 | 122 | 596 |
| Standard Deviation | 1.9 | 7.5 | 22.1 | 3.4 | 6.7 | 25.2 |
| CV% | 4.2 | 5.8 | 3.5 | 7.7 | 5.5 | 4.2 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 34 | 34 | 34 |
| Mean (pg/mL) | 44.4 | 117 | 692 | 45.4 | 128 | 662 |
| Standard Deviation | 2.0 | 7.0 | 25.7 | 4.8 | 7.5 | 35.5 |
| CV% | 4.4 | 6.0 | 3.7 | 10.5 | 5.9 | 5.4 |
Recovery for Mouse/Rat IL-22 Quantikine ELISA Kit
The recovery of IL-22 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=4) | 98 | 93-103 |
| Mouse EDTA Plasma (n=4) | 95 | 86-106 |
| Mouse Heparin Plasma (n=4) | 94 | 83-102 |
| Mouse Serum (n=4) | 91 | 84-99 |
| Rat Cell Culture Supernates (n=4) | 98 | 91-102 |
| Rat EDTA Plasma (n=4) | 97 | 91-111 |
| Rat Heparin Plasma (n=4) | 93 | 88-101 |
| Rat Serum (n=4) | 96 | 90-107 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-22 in each matrix were diluted with the appropriate Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat IL-22 Quantikine ELISA Kit
Mouse/Rat IL-22 ELISA Cell Culture Supernate Standard Curve
Mouse/Rat IL-22 ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-22
Long Name
Interleukin 22
Alternate Names
IL-TIF, IL22
Gene Symbol
IL22
Additional IL-22 Products
Product Documents for Mouse/Rat IL-22 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse/Rat IL-22 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse/Rat IL-22 Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse/Rat IL-22 Quantikine ELISA Kit (M2200):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
120 µL Substrate Solution
120 µL Stop Solution
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