Mouse/Rat M-Cadherin/Cadherin-15 Antibody

  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse M‑Cadherin/Cadherin‑15 in direct ELISAs and mouse and rat M‑Cadherin/Cadherin‑15 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human M-Cadherin, recombinant mouse (rm) Cadherin-13, or rmP-Cadherin is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 800512
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse M‑Cadherin/Cadherin‑15
    Val22-Ala605
    Accession # P33146
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat M‑Cadherin/Cadherin‑15 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and L6 rat myoblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse M‑Cadherin/
Cadherin‑15 Monoclonal Antibody (Catalog # MAB76771) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for M‑Cadherin/Cadherin‑15 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-Cadherin/Cadherin-15

CDH-15 (Cadherin 15; also M-cadherin, Muscle cadherin and Cadherin-14) is a 125-127 kDa atypical member of the classical cadherin family, cadherin superfamily of molecules. It is expressed by muscle satellite cells, cells of the embryonic myotome, and hematopoietic bone marrow stem cells. CDH-15 appears to bind homotypically in trans, thus allowing for the identification and subsequent fusion of myoblast precursors, particularly those in slow-twitch (or red fiber) muscles. This is accompanied by a downregulation of mitochondrial induced apoptosis. Mouse CDH-15 is synthesized as a 784 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, a 38 aa propeptide, and a 725 aa mature region. The mature region is expressed as a type I transmembrane glycoprotein that possesses a 546 aa extracellular region (aa 60-605) and a 159 aa cytoplasmic domain (aa 626-784). The extracellular region shows five consecutive cadherin domains. Over aa 22-605, mouse CDH-15 shares 88% and 97% aa sequence identity with human and rat CDH-15, respectively.

  • Long Name:
    M-Cadherin [Myotubule]
  • Entrez Gene IDs:
    1013 (Human); 12555 (Mouse); 361432 (Rat)
  • Alternate Names:
    cadherin 15, M-cadherin (myotubule); cadherin 15, type 1, M-cadherin (myotubule); Cadherin-14; Cadherin-15; cadherin-3; CDH14; CDH14cadherin-14; CDH15; CDH3cadherin-15; CDHM; MCAD; MCadherin; M-Cadherin; MRD3; Muscle cadherin; muscle-cadherin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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