Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Polyclonal Goat IgG
View all Neuropilin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑1
Phe22-Ala810 (Lys811Arg), Ser829-Asp854
Accession # Q9QWJ9

Specificity

Detects mouse and rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 8% cross-reactivity with recombinant human Neuropilin‑1 is observed, and less than 1% cross-reactivity with recombinant rat Neuropilin-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antibody

Detection of Neuropilin-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Neuropilin‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Neuropilin-1 antibody in PC-12 Rat Cell Line antibody by Flow Cytometry.

Detection of Neuropilin‑1 in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Goat Anti-Mouse/Rat Neuropilin-1 Alexa Fluor® 700-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB566N, filled histogram) or isotype control antibody (Catalog # IC108N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Mouse Neuropilin-1 by Flow Cytometry

Detection of Mouse Neuropilin-1 by Flow Cytometry

pTregs show a higher susceptibility to convert into exFoxp3 cells after transfer into dry eye hosts. pTregs (CD4+CD25+Nrp-1−) and tTregs (CD4+CD25+Nrp-1+) from transgenic dry eye disease (DED) mice (Foxp3-GFP×R26-RFP) were adoptively transferred into control and DED transplant recipients. (A and B) Draining lymph nodes were harvested at day 14 post-transplantation. Single cell suspensions were analyzed by flow cytometry. Frequencies of exFoxp3 cells (GFP−RFP+) from control and DED hosts that received (A) pTregs (59.02 ± 0.92% vs. 78.26 ± 0.69%, n = 4, ***p < 0.0001 unpaired two-tailed Student’s t test) or (B) tTregs was assessed. (C) Representative flow cytometry plots showing frequencies of IL-17 and IFN gamma -expressing exFoxp3 cells in draining lymph nodes from control and dry eye hosts. (D) Nrp1+ tTregs or Nrp1− pTregs from hosts with accepted allografts were adoptively transferred to dry eye hosts, and graft survival was assessed for 8 weeks (n = 10, χ2 = 0.6087, p = 0.4353). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29728574), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Neuropilin-1 by Flow Cytometry

Detection of Mouse Neuropilin-1 by Flow Cytometry

Dry eye disease promotes loss of Foxp3 expression by Tregs following corneal transplantation. Foxp3-GFP-CreXR26-RFP transgenic mice were used to trace Foxp3 expression after transplantation. Healthy corneal grafts were transplanted onto transgenic naïve control hosts or dry eye (DED) hosts. Draining lymph nodes were harvested 2 weeks post-transplantation. Single cell suspensions were analyzed by flow cytometry. (A) Representative flow cytometry plots and (B) statistical analysis of flow cytometry data showing frequencies of exFoxp3 cells in Foxp3-GFPXR26-RFP control and DED hosts (n = 6/group, ***p < 0.001). (C) Representative flow cytometry plots and (D, E) statistical analyses of flow cytometry data showing frequencies of exFoxp3 cells that express IFN gamma (***p < 0.001) and IL-17 (**p < 0.01) in Foxp3-GFPXR26-RFP control and DED hosts (n = 6/group). Data is presented as mean ± SEM (error bar). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29728574), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Neuropilin-1 by Flow Cytometry

Detection of Mouse Neuropilin-1 by Flow Cytometry

Dry eye disease reduces graft survival and Treg function after allograft survival. Healthy corneal grafts were transplanted on normal control hosts or dry eye (DED) hosts. (A) Corneal graft opacity (***p < 0.001) and (B) graft survival were assessed for 8 weeks (n = 10/group, **p < 0.01). Draining lymph nodes were harvested 2 weeks post-transplantation. Single cell suspensions were analyzed by flow cytometry. (C) Frequencies of CD4+Foxp3+ Tregs from control vs. DED hosts was determined using flow cytometry (n = 5 and ***p < 0.001) (D) Treg suppression assay (n = 6) was performed using CD4+CD25− responders from rejected control hosts, and CD4+CD25+Tregs from control and dry eye hosts (**p < 0.01). Data is presented as mean ± SEM (error bar). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29728574), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Optimal dilution of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

5 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line and PC‑12 rat adrenal pheochromocytoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-1

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted semaphorin subfamily as well as some isoforms of VEGF family proteins.

Alternate Names

BDCA-4, CD304, Neuropilin1, NRP1

Entrez Gene IDs

8829 (Human); 18186 (Mouse); 246331 (Rat); 100286859 (Porcine)

Gene Symbol

NRP1

UniProt

Q9QWJ9

Additional Neuropilin-1 Products

Product Documents for Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antibody

* This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for Rat Neuropilin‑1 Alexa Fluor® 700‑conjugated Antibody

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