Mouse/Rat PDGF-BB Quantikine ELISA Kit

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MBB00
PDGF-BB ELISA Kit ELISA
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Procedure
Citations (12)
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Mouse/Rat PDGF-BB Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
19.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat PDGF-BB.  This assay cross-reacts 10.2% with recombinant Rat PDGF-AB, 5.3% with recombinant Human PDGF-AB, 64.7% with recombinant Human PDGF-BB, 38.1% with natural Human PDGF and 65.2% with natural Porcine PDGF.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse/Rat PDGF-BB Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse/rat PDGF-BB in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat PDGF-BB and antibodies raised against recombinant human PDGF-BB. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse/rat PDGF-BB showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse/rat PDGF-BB.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 22 24 24
Mean 49.7 252 823 52.1 246 811
Standard Deviation 4.5 18.7 36.7 5.3 21.1 40.4
CV% 9.1 7.4 4.5 10.2 8.6 5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 22 22 23
Mean 61.1 298 1019 69.5 305 1048
Standard Deviation 5.6 24.6 40.3 6.6 26.3 98.5
CV% 9.2 8.3 4 9.5 8.6 9.4

Recovery

The recovery of PDGF-BB spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Mouse Cell Culture Supernates (n=8) 100 89-118
Mouse Heparin Plasma (n=5) 96 85-113
Rat Cell Culture Supernates (n=8) 102 94-114
Rat EDTA Plasma (n=5) 102 86-111
Rat Heparin Plasma (n=5) 102 85-111
Rat Serum (n=5) 100 89-113

Linearity

To assess the linearity of the assay, samples spiked with or containing PDGF-BB in each matrix were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat PDGF-BB ELISA Linearity
Mouse/Rat PDGF-BB ELISA Linearity

Data Examples

Mouse/Rat PDGF-BB ELISA Cell Culture Supernate Standard Curve

Mouse/Rat PDGF-BB ELISA Serum/Plasma Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-BB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:
Platelet-derived Growth Factor BB
Entrez Gene IDs:
5155 (Human); 24628 (Rat)
Alternate Names:
PDGFBB; PDGF-BB
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat PDGF-BB Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Rosmarinic acid attenuates hepatic fibrogenesis via suppression of hepatic stellate cell activation/proliferation and induction of apoptosis
    Authors: NM El-Lakkany, WH El-Maadawy, SH Seif El-Di, OA Hammam, SH Mohamed, SM Ezzat, MM Safar, S Saleh
    Asian Pac J Trop Med, 2017;10(5):444-453.
    Species: Rat
    Sample Types: Tissue Homogenates
  2. Freeze-Dried Platelet-Rich Plasma Accelerates Bone Union with Adequate Rigidity in Posterolateral Lumbar Fusion Surgery Model in Rats
    Sci Rep, 2016;6(0):36715.
    Species: Rat
    Sample Types: Plasma
  3. Integration of growth factor gene delivery with collagen-triggered wound repair cascades using collagen-mimetic peptides
    Authors: Morgan A Urello
    Bioeng Transl Med, 2016;1(2):207-219.
    Species: Mouse
    Sample Types: Complex Sample Type
  4. The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages
    Nat Commun, 2016;7(0):11385.
    Species: Mouse
    Sample Types: Cell Lysates
  5. Treatment of refractory cutaneous ulcers with mixed sheets consisting of peripheral blood mononuclear cells and fibroblasts
    Authors: Koji Ueno
    Sci Rep, 2016;6(0):28538.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Uncoupling between inflammatory and fibrotic responses to silica: evidence from MyD88 knockout mice.
    Authors: Re S, Giordano G, Yakoub Y, Devosse R, Uwambayinema F, Couillin I, Ryffel B, Marbaix E, Lison D, Huaux F
    PLoS ONE, 2014;9(7):e99383.
    Species: Mouse
    Sample Types: BALF
  7. Pulsed estrogen therapy prevents post-OVX porcine dura mater microvascular network weakening via a PDGF-BB-dependent mechanism.
    Authors: Glinskii, Olga V, Huxley, Virginia, Glinskii, Vladimir, Rubin, Leona J, Glinsky, Vladisla
    PLoS ONE, 2013;8(12):e82900.
    Species: Mouse
    Sample Types: Cell Lysates
  8. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011;121(11):4548-66.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Statins restore ischemic limb blood flow in diabetic microangiopathy via eNOS/NO upregulation but not via PDGF-BB expression.
    Authors: Fujii T, Onimaru M, Yonemitsu Y, Kuwano H, Sueishi K
    Am. J. Physiol. Heart Circ. Physiol., 2008;294(6):H2785-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. The role of the receptor for advanced glycation end-products in lung fibrosis.
    Authors: He M, Kubo H, Ishizawa K, Hegab AE, Yamamoto Y, Yamamoto H, Yamaya M
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(6):L1427-36.
    Species: Mouse
    Sample Types: BALF
  11. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Types: Plasma
  12. Pharmacological inhibition of epsilon PKC suppresses chronic inflammation in murine cardiac transplantation model.
    Authors: Koyanagi T, Noguchi K, Ootani A, Inagaki K, Robbins RC, Mochly-Rosen D
    J. Mol. Cell. Cardiol., 2007;43(4):517-22.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Mouse/Rat PDGF-BB Quantikine ELISA Kit
By Anonymous on 06/19/2018
Sample Tested: Serum