Mouse/Rat Semaphorin 4C Antibody Summary
Accession # Q64151
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Semaphorin 4C by Western Blot (
Semaphorin 4C in Rat Cortical Stem Cells. Semaphorin 4C was detected in immersion fixed rat cortical stem cells using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Semaphorin 4C in Mouse Embryo. Semaphorin 4C was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes of the developing cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat Semaphorin 4C by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue (15 d.p.c.), loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 117 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 4C
Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.
Citations for Mouse/Rat Semaphorin 4C Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Plexin B2 and Semaphorin 4C Guide T Cell Recruitment and Function in the Germinal Center
Authors: H Yan, L Wu, C Shih, S Hou, J Shi, T Mao, W Chen, B Melvin, RJ Rigby, Y Chen, H Jiang, RH Friedel, CG Vinuesa, H Qi
Cell Rep, 2017;19(5):995-1007.
Sample Types: Whole Cells
Applications: Flow Cytometry
Semaphorin 4C Protects against Allergic Inflammation: Requirement of Regulatory CD138+ Plasma Cells
Authors: Bruce D Mazer
J. Immunol., 2016;0(0):.
Sample Types: Cell Lysates
Applications: Western Blot
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