Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Semaphorin 4C Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4C
Glu22-Gly664
Accession # Q64151

Specificity

Detects mouse and rat Semaphorin 4C in Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant human Semaphorin 4C and less than 5% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4D, and rmSemaphorin 4G is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse/Rat Semaphorin 4C Antibody

Detection of Mouse Semaphorin 4C antibody by Western Blot (Non-Reducing).

Detection of Mouse Semaphorin 4C by Western Blot (Non-Reducing).

Western blot shows lysates of mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4C at approximately 240 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Semaphorin 4C antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Semaphorin 4C in Rat Cortical Stem Cells.

Semaphorin 4C was detected in immersion fixed rat cortical stem cells using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Semaphorin 4C antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Semaphorin 4C in Mouse Embryo.

Semaphorin 4C was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes of the developing cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat Semaphorin 4C antibody by Simple WesternTM.

Detection of Mouse and Rat Semaphorin 4C by Simple WesternTM.

Simple Western lane view shows lysates of mouse embryo tissue (15 d.p.c.), loaded at 0.2 mg/mL. A specific band was detected for Semaphorin 4C at approximately 117 kDa (as indicated) using 50 µg/mL of Sheep Anti-Mouse/Rat Semaphorin 4C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6120) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Mouse/Rat Semaphorin 4C Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed rat cortical stem cells

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (13 d.p.c.)

Simple Western

50 µg/mL
Sample: Mouse embryo tissue (15 d.p.c.)

Western Blot

1 µg/mL
Sample: Mouse embryo (15 d.p.c.) tissue

Reviewed Applications

Read 1 review rated 3 using AF6120 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Alternate Names

SEMA4C, SEMACL1, Semaf, SEMAI

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Gene Symbol

SEMA4C

UniProt

Q64151

Additional Semaphorin 4C Products

Product Documents for Mouse/Rat Semaphorin 4C Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat Semaphorin 4C Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse/Rat Semaphorin 4C Antibody

Customer Reviews for Mouse/Rat Semaphorin 4C Antibody (1)

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  • Mouse/Rat Semaphorin 4C Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Splenic B cells
    Species: Mouse
    Verified Customer | Posted 07/27/2016

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Protocols

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