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Mouse ROBO4 MAb (Clone 274940)

Catalog #: MAB5004 Datasheet / COA / SDS

Discontinued Product

MAB5004 has been discontinued.
View all ROBO4 products.
Detection of ROBO4 in bEnd.3 Mouse Cell Line by Flow Cytometry.
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Mouse ROBO4 MAb (Clone 274940) Summary

Species Reactivity
Mouse
Specificity
Detects mouse ROBO4. Stains mouse ROBO4 transfectants but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 274940
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with mouse ROBO4
aa 28-1012
Accession # Q8C310
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ROBO4 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of ROBO4 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse ROBO4 Monoclonal Antibody (Catalog # MAB5004, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO4

Members of the ROBO family are a group of highly conserved transmembrane glycoproteins that make up a small subgroup of the immunoglobulin (Ig) superfamily. They are characterized structurally by five C2-type Ig-like repeats, three fibronectin type III domains, a transmembrane region, and an intracellular domain with three (ROBO3) or four (ROBO1, 2) CC (conserved cytoplasmic) motifs. ROBO4, a fourth putative family member, has been identified, but lacks one of the three fibronectin-like domains and two of the five Ig-like domains. ROBOs are best known for their roles as receptors for the Slit family of repellent axon guidance cues.

Long Name
Roundabout Homolog 4
Entrez Gene IDs
54538 (Human); 74144 (Mouse)
Alternate Names
ECSM4; FLJ20798; Magic roundabout; MGC133352; MGC133353; MRB; ROBO4; roundabout homolog 4; roundabout homolog 4, magic roundabout (Drosophila)

Product Datasheets

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Product Datasheet
COA
SDS

FAQs

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