Mouse S100A11 Antibody

Catalog # Availability Size / Price Qty
MAB5167
MAB5167-SP
Product Details
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Mouse S100A11 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A11 in direct ELISAs and Western blots. In direct ELISAs, ;no cross-reactivity with recombinant mouse S100A1, 4, 6, 8, 9, 10, 13, or 16 is observed, and approximately 75% cross‑reactivity with recombinant human (rh) S100A11 is observed. In Western blots, no cross‑reactivity with rhS100A11 is observed
Source
Monoclonal Rat IgG2A Clone # 493804
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse S100A11
Met1-Ile98
Accession # P50543
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse S100A11

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A11

S100A11, also called calgizzarin or S100C, is an antiproliferative 10 kDa protein that is a member of the S100 family of EF-hand calcium binding proteins. It is found in the cytosol of proliferating fibroblasts and keratinocytes, but in the nucleus in differentiating cells. TGF-beta treatment or calcium-induced S100A11 serine phosphorylation induces nuclear translocation of S100A11 and subsequent growth inhibition in normal cells. The 98 amino acid (aa) mouse S100A11 shares 82% and 94% aa identity with human and rat S100A11, respectively.

Long Name
S100 Calcium Binding Protein A11
Entrez Gene IDs
6282 (Human); 20195 (Mouse); 445415 (Rat)
Alternate Names
Calgizzarin; Metastatic lymph node gene 70 protein; MLN 70; MLN70; protein S100-A11; Protein S100-C; S100 calcium binding protein A11; S100 calcium-binding protein A11 (calgizzarin); S100 calcium-binding protein A11; S100A11; S100C; S100CS100 calcium binding protein A11 (calgizzarin)

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