Mouse S100A9 Antibody

(9 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S100A9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A10, rhS100B, or rhS100P is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 372510
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse S100A9
    Met1-Lys113
    Accession # P31725
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse S100A9 by Western Blot. Western blot shows lysates of mouse leukocytes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A9 at approximately 14 kDa (as indicated). This experiment was conducted under reducing (R) and non-reducing (NR) conditions and using Immunoblot Buffer Group 1.
Detection of Mouse S100A9 by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A9 at approximately 19 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A9

S100A9, also known as calgranulin B or migration inhibitory factor-related protein 14 (MRP14), belongs to the S100 family of calcium binding proteins of the EF-hand type. It is highly expressed in myeloid cells and other cell types including keratinocytes, epithelial cells and endothelial cells. S100A9 is a cytosolic protein that can also be released into the extracellular milieu. It is associated with various inflammatory diseases.

  • Long Name:
    S100 Calcium Binding Protein A9
  • Entrez Gene IDs:
    6280 (Human); 20202 (Mouse)
  • Alternate Names:
    CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. A Critical Role of Zinc Importer AdcABC in Group A Streptococcus-Host Interactions During Infection and Its Implications for Vaccine Development
    Authors: N Makthal, K Nguyen, H Do, M Gavagan, P Chandrangs, JD Helmann, RJ Olsen, M Kumaraswam
    EBioMedicine, 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Interleukin 6 regulates psoriasiform inflammation-associated thrombosis
    JCI Insight, 2016;1(20):e89384.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. The calcium-binding protein complex S100A8/A9 has a crucial role in controlling macrophage-mediated renal repair following ischemia/reperfusion.
    Authors: Dessing, Mark C, Tammaro, Alessand, Pulskens, Wilco P, Teske, Gwendoli, Butter, Loes M, Claessen, Nike, van Eijk, Marco, van der Poll, Tom, Vogl, Thomas, Roth, Johannes, Florquin, Sandrine, Leemans, Jaklien
    Kidney Int, 2015;87(1):85-94.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. RAGE expression in tumor-associated macrophages promotes angiogenesis in glioma.
    Authors: Chen X, Zhang L, Zhang I, Liang J, Wang H, Ouyang M, Wu S, da Fonseca A, Weng L, Yamamoto Y, Yamamoto H, Natarajan R, Badie B
    Cancer Res, 2014;74(24):7285-97.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. ID1 promotes breast cancer metastasis by S100A9 regulation.
    Authors: Gumireddy K, Li A, Kossenkov A, Cai K, Liu Q, Yan J, Xu H, Showe L, Zhang L, Huang Q
    Mol Cancer Res, 2014;12(9):1334-43.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Proteomic analysis of the role of S-nitrosoglutathione reductase in lipopolysaccharide-challenged mice.
    Proteomics, 2012;12(12):2024-35.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012;7(9):e46311.
    Species: Mouse
    Sample Type: Vaginal Lavage
    Application: ELISA Development
  8. S100A8/A9 is not involved in host defense against murine urinary tract infection.
    Authors: Dessing MC, Butter LM, Teske GJ
    PLoS ONE, 2010;5(10):e13394.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
    Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
    J. Immunol., 2008;181(10):7420-7.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
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Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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