Mouse S1P1/EDG-1 Alexa Fluor® 405-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
  • Source
    Monoclonal Rat IgG2a Clone # 713412
  • Immunogen
    Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
    Accession # O08530
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: S1P1/EDG-1
S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.
  • Long Name:
    Endothelial Differentiation Gene 1
  • Entrez Gene IDs:
    1901 (Human); 13609 (Mouse)
  • Alternate Names:
    CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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