Mouse S1P1/EDG-1 Alexa Fluor® 647-conjugated Antibody Summary
Accession # O08530
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB7089R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and GFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB7089R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.
Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*, be carried out at 4°C?
There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend. Staining for 45 minutes is optimal with this antibody.
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