Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7089G-100ug
Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody in Flow Cytometry
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Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. View Larger

Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7089G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

Product Datasheets

FAQs

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Reviews for Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody

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Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody
By Anonymous on 07/20/2018
Application: Flow Sample Tested: Peripheral blood leukocytes Species: Mouse

When used at a 1:10 dilution this antibody can reliably be used to detected expression of S1P1 on peripheral blood lymphocytes. When I tired to used this antibody at a lower concentration (higher dilution) I was not able to detect staining. Thus, a large amount of antibody is needed to stain each sample.