Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 713412
View all S1P1/EDG-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530

Specificity

Detects mouse S1P

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody

Detection of S1P1/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of S1P1/EDG-1 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7089G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (IC006G). View our protocol for Staining Membrane-associated Proteins.

Detection of S1P1/EDG‑1 in mEDG-1 HEK transfectants by Flow Cytometry

Rat Anti-Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7089G) detection in mEDG-1 HEK/mCherry transfectants (A) or irrelevant mCherry transfectants (B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Mouse thymocytes; transfectants

Reviewed Applications

Read 1 review rated 3 using FAB7089G in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitution default not known

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

O08530

Additional S1P1/EDG-1 Products

Product Documents for Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews (1)

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  • Mouse S1P1/EDG-1 Alexa Fluor® 488-conjugated Antibody
    Name: Colleen Netherby
    Application: Flow Cytometry
    Sample Tested: Peripheral blood leukocytes
    Species: Mouse
    Verified Customer | Posted 07/20/2018
    When used at a 1:10 dilution this antibody can reliably be used to detected expression of S1P1 on peripheral blood lymphocytes. When I tired to used this antibody at a lower concentration (higher dilution) I was not able to detect staining. Thus, a large amount of antibody is needed to stain each sample.
    Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody FAB7089G

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FAQs for Mouse S1P1/EDG‑1 Alexa Fluor® 488‑conjugated Antibody

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  • Q: Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*,  be carried out at 4°C? 

    A: There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend.  Staining for 45 minutes is optimal with this antibody. 

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