Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 713412
View all S1P1/EDG-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530

Specificity

Detects mouse S1P

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

Detection of S1P1/EDG-1 antibody in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP antibody by Flow Cytometry.

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P1/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry.

Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry.

Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB7089A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP and mouse thymocytes

Reviewed Applications

Read 2 reviews rated 4 using FAB7089A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

O08530

Additional S1P1/EDG-1 Products

Product Documents for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

For research use only

Citations for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

Customer Reviews for Mouse S1P1/EDG‑1 APC‑conjugated Antibody (2)

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2 Customer Ratings
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  • Mouse S1P1/EDG-1 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 10/25/2018
  • Mouse S1P1/EDG-1 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Adult splenocytes, bone marrow and Adult thymocytes
    Species: Mouse
    Verified Customer | Posted 08/24/2017

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FAQs for Mouse S1P1/EDG‑1 APC‑conjugated Antibody

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  • Q: Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*,  be carried out at 4°C? 

    A: There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend.  Staining for 45 minutes is optimal with this antibody. 

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