Mouse S1P1/EDG-1 Antibody

Catalog # Availability Size / Price Qty
MAB7089
MAB7089-SP
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry.
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Product Details
Citations (12)
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Mouse S1P1/EDG-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry S1P1/EDG‑1 in Mouse Heart. View Larger

S1P1/EDG‑1 in Mouse Heart. S1P1/EDG‑1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG‑1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter­stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

Product Datasheets

Citations for Mouse S1P1/EDG-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
    Authors: MJ Lang, M Mori, J Ruer-Laven, J Pieters
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State
    Authors: A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. CD8?? intraepithelial lymphocytes arise from two main thymic precursors
    Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. Ageing adversely affects the migration and function of marginal zone B cells
    Authors: VM Turner, NA Mabbott
    Immunology, 2017;151(3):349-362.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  5. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells
    Authors: A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  6. Thymic progenitors of TCR??(+) CD8?? intestinal intraepithelial lymphocytes require RasGRP1 for development
    Authors: DP Golec, RE Hoeppli, LM Henao Cavi, J McCann, MK Levings, TA Baldwin
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  7. IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
    Authors: Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K
    J Immunol, 2015;195(4):1408-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  8. Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
    Authors: Xu, Shengli, Ou, Xijun, Huo, Jianxin, Lim, Kristen, Huang, Yuhan, Chee, Sheena, Lam, Kong-Pen
    Nat Commun, 2015;6(0):6764.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  9. Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors.
    Authors: Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
    J Exp Med, 2014;211(13):2583-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  10. Moesin controls clathrin-mediated S1PR1 internalization in T cells.
    Authors: Nomachi, Akira, Yoshinaga, Masanori, Liu, Jaron, Kanchanawong, Pakorn, Tohyama, Kiyoshi, Thumkeo, Dean, Watanabe, Takeshi, Narumiya, Shuh, Hirata, Takako
    PLoS ONE, 2013;8(12):e82590.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  11. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
    Authors: Arnon, Tal I, Horton, Robert M, Grigorova, Irina L, Cyster, Jason G
    Nature, 2013;493(7434):684-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  12. The sphingosine 1-phosphate receptor S1P(2) maintains the homeostasis of germinal center B cells and promotes niche confinement.
    Authors: Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG
    Nat. Immunol., 2011;12(7):672-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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