Mouse S1P1/EDG‑1 Antibody
R&D Systems | Catalog # MAB7089
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 713412
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Product Specifications
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Accession # O08530
Specificity
Detects mouse S1P
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse S1P1/EDG‑1 Antibody
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry.
Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
S1P1/EDG‑1 in Mouse Heart.
S1P1/EDG-1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of S1P1/EDG-1 by Flow Cytometry
CD69 and S1PR1 expression on CD4 T cells before and following cell transfer. Activated OT-II T cells were analyzed before cell transfer (A) for expression of CD69 and S1PR1 or for CD69 expression ex vivo 24 h after transfer into resting (PBS) or inflamed [lipopolysaccharide (LPS)] ear pinnae (B,C). Before transfer, the OT-II T cells were treated with an S1PR1 antagonist or agonist as in Figure 5B. In panel (A), cells are gated on live CD4+ CD45+ cells; representative of three independent samples. In panels (B,C), data are combined from two to three independent experiments with ≥3 animals per group. In this figure, p < 0.05 is indicated by * and p < 0.001 by **. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29225602), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse S1P1/EDG‑1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP, and mouse thymocytes
Sample: HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG‑1 and GFP, and mouse thymocytes
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse heart
Sample: Perfusion fixed frozen sections of mouse heart
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: S1P1/EDG-1
Long Name
Endothelial Differentiation Gene 1
Alternate Names
CD363, EDG1, S1PR1
Gene Symbol
S1PR1
UniProt
Additional S1P1/EDG-1 Products
Product Documents for Mouse S1P1/EDG‑1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse S1P1/EDG‑1 Antibody
For research use only
Citations for Mouse S1P1/EDG‑1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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