Mouse Sca-1/Ly6 PE-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Sca‑1/Ly6 in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 177228
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
    Leu27-Gly119
    Accession # CAA28351
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit APC‑conjugated Monoclonal Antibody (Catalog # FAB1356A) and either (A) Rat Anti-Mouse Sca‑1/Ly6 PE‑conjugated Monoclonal Antibody (Catalog # FAB1226P) or (B) Rat IgG2A Phycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Sca-1/Ly6

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

  • References:
    1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
    2. Spangrude, G.I. et al. (1988) Science 241:58.
    3. Spangrude, G.I. et al. (1993) Blood 82:3327.
    4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
    5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
    6. Yamamoto, Y. et al. (1996, Blood 88:445.
    7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
    8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
    9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
    10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
    11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
    12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.
  • Long Name:
    Stem Cell Antigen-1/Lymphocyte Antigen 6
  • Entrez Gene IDs:
    110454 (Mouse)
  • Alternate Names:
    Ly6; Ly-6; Sca1; Sca-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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