Detection of Sca‑1/Ly6 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Sca‑1/Ly6 in Mouse Splenocytes. Sca‑1/Ly6 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse Sca‑1/Ly6 Monoclonal Antibody (Catalog # MAB1226) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).
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R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
BCL6 is critical for the development of a diverse primary B cell repertoire. Authors: Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jack HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alboran IM, Melnick AM, Ye BH, Muschen M J. Exp. Med., 2010;207(6):1209-21. Species: Mouse Sample Type: Whole Cells Application: Flow
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