Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2A Clone # 177228
View all Sca-1/Ly6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351

Specificity

Detects mouse Sca-1/Ly6 in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Sca‑1/Ly6 Biotinylated Antibody

Sca‑1/Ly6 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr).

Sca‑1/Ly6 in Mouse Kidney.

Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse kidney using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Sca-1/Ly6 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

Sca‑1/Ly6 in Mouse Thymus.

Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Sca‑1/Ly6 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse thymus and mouse kidney

Western Blot

1 µg/mL
Sample: Recombinant Mouse Sca‑1/Ly6
under non-reducing conditions only

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Sca-1/Ly6

Sca-1 is an 18 kDa phosphatidylositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2,3). Sca-1-positive HSC can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal (2-7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9-12).

References

  1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
  2. Spangrude, G.I. et al. (1988) Science 241:58.
  3. Spangrude, G.I. et al. (1993) Blood 82:3327.
  4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
  5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
  6. Yamamoto, Y. et al. (1996) Blood 88:445.
  7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
  8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
  9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
  10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
  11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.

Long Name

Stem Cell Antigen-1/Lymphocyte Antigen 6

Alternate Names

Ly-6, Ly6, Sca1

Entrez Gene IDs

110454 (Mouse)

Gene Symbol

LY6A

UniProt

CAA28351

Additional Sca-1/Ly6 Products

Product Documents for Mouse Sca‑1/Ly6 Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Sca‑1/Ly6 Biotinylated Antibody

For research use only

Citations for Mouse Sca‑1/Ly6 Biotinylated Antibody

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Protocols

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