Mouse Sca-1/Ly6 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1226
Sca‑1/Ly6 in Mouse Kidney.
2 Images
Product Details
Citations (5)
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Mouse Sca-1/Ly6 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Sca-1/Ly6 in Western blots.
Source
Monoclonal Rat IgG2A Clone # 177228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
Leu27-Gly119
Accession # CAA28351
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Sca‑1/Ly6
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Sca‑1/Ly6 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

Sca‑1/Ly6 in Mouse Kidney. Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse kidney using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry Sca-1/Ly6 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Sca‑1/Ly6 in Mouse Thymus. Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sca-1/Ly6

Sca-1 is an 18 kDa phosphatidylositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2,3). Sca-1-positive HSC can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal (2-7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9-12).

References
  1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
  2. Spangrude, G.I. et al. (1988) Science 241:58.
  3. Spangrude, G.I. et al. (1993) Blood 82:3327.
  4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
  5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
  6. Yamamoto, Y. et al. (1996) Blood 88:445.
  7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
  8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
  9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
  10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
  11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
Long Name
Stem Cell Antigen-1/Lymphocyte Antigen 6
Entrez Gene IDs
110454 (Mouse)
Alternate Names
C6orf23; G6D; Ly6; Ly-6; LY6-D; LY6G6D; lymphocyte antigen 6 family member G6D; MEGT1; NG25; Sca1; Sca-1

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Citations for Mouse Sca-1/Ly6 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate
    Authors: Hansen, SL;Larsen, HL;Pikkupeura, LM;Maciag, G;Guiu, J;Müller, I;Clement, DL;Mueller, C;Johansen, JV;Helin, K;Lerdrup, M;Jensen, KB;
    Science advances
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  2. Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis
    Authors: JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom
    Nature Communications, 2021-06-08;12(1):3464.
    Species: Mouse
    Sample Types: Organoid
    Applications: IHC
  3. Characterization of radioresistant epithelial stem cell heterogeneity in the damaged mouse intestine
    Authors: T Sato, M Sase, S Ishikawa, M Kajita, J Asano, T Sato, Y Mori, T Ohteki
    Sci Rep, 2020-05-22;10(1):8308.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
    Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
    Cell Stem Cell, 2017-12-14;22(1):35-49.e7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments.
    Authors: Sergejeva S, Johansson AK, Malmhall C, Lotvall J
    Blood, 2003-09-25;103(4):1270-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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