Mouse SCARA5 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF4754
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Mouse SCARA5 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SCARA5 in Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human SCARA5 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse SCARA5
Arg83-Pro491
Accession # Q8K299
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SCARA5 (Catalog # 4754-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SCARA5

Scavenger receptor A5 (SCARA5; also known as testis expressed scavenger receptor Tesr) is a Class A scavenger receptor that is related to CL-P1, MARCO, SCARA3, and SR-A1. All are type II transmembrane proteins that contain a collagenous stalk (1‑3). Mature mouse SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 410 aa C-terminal extracellular domain (ECD) that contains a coiled-coil, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) region (4, 5). Within the ECD, mouse SCARA5 shares 87% and 98% aa sequence identity with human and rat SCARA5, respectively. It shares 24%‑29% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternative splicing generates one isoform that lacks the SRCR domain and a second that additionally lacks the cytoplasmic and transmembrane regions. SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

References
  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
  3. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  4. Sarraj, M.A. et al. (2002) Dev. Dyn. 234:1026.
  5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.
Long Name
Scavenger Receptor Class A Member 5
Entrez Gene IDs
286133 (Human); 71145 (Mouse); 305974 (Rat)
Alternate Names
FLJ23907; MGC45780; SCARA5; scavenger receptor class A, member 5 (putative); Tesr; testis expressed scavenger receptor; UNQ2938/PRO28700

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