Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SCARA5 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse SCARA5
Arg83-Pro491
Accession # Q8K299

Specificity

Detects mouse SCARA5 in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human SCARA5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse SCARA5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SCARA5 (Catalog # 4754-SR)

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCARA5

Scavenger receptor A5 (SCARA5; also known as testis expressed scavenger receptor Tesr) is a Class A scavenger receptor that is related to CL-P1, MARCO, SCARA3, and SR-A1. All are type II transmembrane proteins that contain a collagenous stalk (1‑3). Mature mouse SCARA5 consists of a 60 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 410 aa C-terminal extracellular domain (ECD) that contains a coiled-coil, a collagen-like, and a scavenger receptor cysteine-rich (SRCR) region (4, 5). Within the ECD, mouse SCARA5 shares 87% and 98% aa sequence identity with human and rat SCARA5, respectively. It shares 24%‑29% aa sequence identity with CL-P1, MARCO, SCARA3, and SR-A1. Alternative splicing generates one isoform that lacks the SRCR domain and a second that additionally lacks the cytoplasmic and transmembrane regions. SCARA5 is a cell surface disulfide-linked homotrimer of > 250 kDa (5). It is highly expressed in testicular Sertoli and germ cells and more weakly in the epithelia of other tissues (4, 5). During mouse development, SCARA5 expression is attenuated in female embryos at the time of sex determination, whereas it is maintained in the developing testis (4). SCARA5 is re-expressed in the adult ovary (4). SCARA5 binds heat-killed bacterial particles but not yeast particles or modified LDL (5).

References

  1. Murphy, J.E. et al. (2005) Atherosclerosis 182:1.
  2. Mukhopadhyay, S. And S. Gordon (2004) Immunobiology 209:39.
  3. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  4. Sarraj, M.A. et al. (2002) Dev. Dyn. 234:1026.
  5. Jiang, Y. et al. (2006) J. Biol. Chem. 281:11834.

Long Name

Scavenger Receptor Class A Member 5

Alternate Names

Tesr

Entrez Gene IDs

286133 (Human); 71145 (Mouse); 305974 (Rat)

Gene Symbol

SCARA5

UniProt

Q8K299

Additional SCARA5 Products

Product Documents for Mouse SCARA5 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse SCARA5 Antibody

For research use only

Related Research Areas

Primordial and Mature Germ Cells

Citations for Mouse SCARA5 Antibody

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