Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 428208
View all SCD-1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse SCD-1
Met1-Ser355
Accession # P13516

Specificity

Detects mouse SCD-1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse SCD‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse SCD‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCD-1

Stearoyl-CoA Desaturase 1 (SCD-1) is a four-transmembrane endoplasmic reticulum protein that is a rate-limiting enzyme in the formation of triglycerides, cholesteryl esters and phospholipids from saturated fatty acids. Its expression in adipose and liver is enhanced by insulin and certain dietary sugars and fats, and down‑regulated by TNF-alpha. SCD-1 is one of four iron-containing murine stearoyl-CoA desaturases that share 79‑88% aa identity. The 355 aa, predicted 41 kDa mouse SCD-1 shares 92% and 84% aa identity with rat and human SCD-1, respectively.

Long Name

Stearoyl CoA-desaturase 1, Isoform of the Delta9 Desaturase

Alternate Names

SCD1

Entrez Gene IDs

6319 (Human); 20249 (Mouse)

Gene Symbol

SCD

UniProt

P13516

Additional SCD-1 Products

Product Documents for Mouse SCD‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse SCD‑1 Antibody

For research use only

Related Research Areas

Redox Enzymes

Citations for Mouse SCD‑1 Antibody

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