Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 256537
View all Semaphorin 6B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 6B
Leu27-Ser605 (predicted)
Accession # O54951

Specificity

Detects mouse Semaphorin 6B in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant human (rh) Semaphorin 6B is observed and no cross-reactivity with rhSemaphorin 3A, recombinant mouse Semaphorin 3B, 3C, 3E, 3F, 6A, 6C, 6D, or 7A is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse Semaphorin 6B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Semaphorin 6B

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 6B

Semaphorin 6B is a type I transmembrane protein belonging to the Semaphorin family. It is highly expressed in human brain and is expressed at lower levels in other tissues. A splice variant of Semaphorin 6B encodes a secreted protein.

Alternate Names

Sema6B

Entrez Gene IDs

10501 (Human); 20359 (Mouse)

Gene Symbol

SEMA6B

UniProt

O54951

Additional Semaphorin 6B Products

Product Documents for Mouse Semaphorin 6B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Semaphorin 6B Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Mouse Semaphorin 6B Antibody

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