Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Serpin E1/PAI-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI‑1
Leu24-Ser402
Accession # AAH54091

Specificity

Detects mouse Serpin E1/PAI‑1 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant human (rh) Serpin E1 is observed, and less than 2% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serpin E1/PAI‑1 Biotinylated Antibody

Detection of Mouse Serpin E1/PAI-1 antibody by Western Blot.

Detection of Mouse Serpin E1/PAI‑1 by Western Blot.

Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3828) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions.

Applications for Mouse Serpin E1/PAI‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample:   Mouse placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Gene Symbol

SERPINE1

UniProt

AAH54091

Additional Serpin E1/PAI-1 Products

Product Documents for Mouse Serpin E1/PAI‑1 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Serpin E1/PAI‑1 Biotinylated Antibody

For research use only

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FAQs

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail