Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin E1/PAI-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E1/PAI-1
Leu24-Ser402
Accession # AAH54091

Specificity

Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human Serpin E1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Serpin E1/PAI‑1 Antibody

Detection of Mouse Serpin E1/PAI-1 antibody by Western Blot.

Detection of Mouse Serpin E1/PAI‑1 by Western Blot.

Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Mouse Serpin E1/PAI-1 antibody by Simple WesternTM.

Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM.

Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Serpin E1/PAI-1 by Western Blot

Detection of Serpin E1/PAI-1 by Western Blot

In vivo expression of exogenous Wnt1 before IR downregulates renal Wnt/ beta -catenin target genes in mice after AKI-CKD progression. (A,B) mRNA expression of PAI-1 and MMP-7 in different groups as indicated. (C–E) Representative Western blot analyses of PAI-1 and Klotho protein levels. (F) mRNA expression of Klotho. *P < 0.05; **P < 0.01. n = 5. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34819873), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Serpin E1/PAI-1 by Western Blot

Detection of Mouse Serpin E1/PAI-1 by Western Blot

Serpine1 expression in TGF-beta –treated NMuMG cells. Relative levels of mRNA Serpine1 (A) and protein SERPINE1 (B) in cells treated with TGF-beta at the indicated times. A representative immunoblotting of SERPINE1 is shown. Quantification of Serpine1 and SERPINE1 in three independent experiments is shown. Error bars represent S.D. ***p < 0.001, **p < 0.01 by two-tailed Student´s t test. Protein-loading normalization was performed by measuring total protein directly on the membrane using the criterion stain-free gel imaging system. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41420-024-01886-8), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Serpin E1/PAI‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E1/PAI‑1, see our available Western blot detection antibodies

Simple Western

12.5 µg/mL
Sample: Mouse placenta tissue

Western Blot

0.25 µg/mL
Sample: Mouse placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin E1/PAI-1

Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse)

Gene Symbol

SERPINE1

UniProt

AAH54091

Additional Serpin E1/PAI-1 Products

Product Documents for Mouse Serpin E1/PAI‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Serpin E1/PAI‑1 Antibody

For research use only

Citations for Mouse Serpin E1/PAI‑1 Antibody

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