Detects mouse Serpin E1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human Serpin E1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Serpin E2 and rmSerpin I1 is observed.
Detection of Mouse Serpin E1/PAI‑1 by Western Blot.
Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI‑1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM.
Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin E1/PAI-1
Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.
Plasminogen Activator Inhibitor
Entrez Gene IDs:
5054 (Human); 18787 (Mouse)
Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1
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