Mouse Serpin E1/PAI-1 Antibody Summary
Leu24-Ser402
Accession # AAH54091
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse Serpin E1/PAI‑1 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin E1/PAI-1 at approximately 45-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Mouse Serpin E1/PAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI-1 at approximately 56 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Mouse Serpin E1/PAI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3828) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin E1/PAI-1
Serpin E1, also known as Plasminogen Activator Inhibitor-1 (PAI-1), is the principal inhibitor of urokinase-type and tissue-type plasminogen activators. It plays a major role in many processes such as angiogenesis, tumor invasion, and obesity. The amino acid sequence of mouse PAI-1 is 89%, 79% and 76% identical to that of rat, human/chimpanzee and dog.
Product Datasheets
Citations for Mouse Serpin E1/PAI-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Reduced secretion of neuronal growth regulator 1 contributes to impaired adipose-neuronal crosstalk in obesity
Authors: E Duregotti, CM Reumiller, U Mayr, M Hasman, LE Schmidt, SA Burnap, K Theofilato, J Barallobre, A Beran, M Grandoch, A Viviano, M Jahangiri, M Mayr
Nature Communications, 2022-11-25;13(1):7269.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Acquired Deficiency of Peroxisomal Dicarboxylic Acid Catabolism is a Metabolic Vulnerability in Hepatoblastoma
Authors: H Wang, J Lu, X Chen, M Schwalbe, JE Gorka, JA Mandel, J Wang, ES Goetzman, S Ranganatha, SF Dobrowolsk, EV Prochownik
The Journal of Biological Chemistry, 2021-01-13;0(0):100283.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells
Authors: J Miao, J Huang, C Luo, H Ye, X Ling, Q Wu, W Shen, L Zhou
Physiological Reports, 2021-01-01;9(2):e14696.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
C-X-C motif chemokine receptor 4 aggravates renal fibrosis through activating JAK/STAT/GSK3&beta/&beta-catenin pathway
Authors: Y Liu, Q Feng, J Miao, Q Wu, S Zhou, W Shen, Y Feng, FF Hou, Y Liu, L Zhou
J. Cell. Mol. Med., 2020-03-02;0(0):.
Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot -
Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction
Authors: J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou
Aging Cell, 2019-07-18;0(0):e13004.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Thymosin beta4 and its degradation product, Ac-SDKP, are novel reparative factors in renal fibrosis.
Authors: Zuo Y, Chun B, Potthoff S, Kazi N, Brolin T, Orhan D, Yang H, Ma L, Kon V, Myohanen T, Rhaleb N, Carretero O, Fogo A
Kidney Int, 2013-06-05;84(6):1166-75.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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