Mouse Serpin E2/PN1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2175
Product Details
FAQs
Supplemental Products
Reviews

Mouse Serpin E2/PN1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin E2/PN1 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Fas, recombinant mouse Fas, and recombinant feline Fas (under non-reducing conditions) is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
Ser20-Pro397
Accession # Q07235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin E2/PN1

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
  3. Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
  4. Meins, M. et al. (2001) J. Neurosci. 21:8830.
  5. Buchholz, M. et al. (2003) Cancer Res. 63:4945.
  6. Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.
Entrez Gene IDs
5270 (Human); 20720 (Mouse)
Alternate Names
GDN; GdNPF; GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2; glia-derived nexin; glial-derived neurite promoting factor; Peptidase inhibitor 7; PI-7; PI7DKFZp686A13110; PN-1; PN1nexin; PNI; Protease nexin 1; Protease nexin I; Proteinase Nexin 1; Serpin E2; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse Serpin E2/PN1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Serpin E2/PN1 Biotinylated Antibody and earn rewards!

Have you used Mouse Serpin E2/PN1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review