Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin E2/PN1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin E2/PN1
Ser20-Pro397
Accession # Q07235

Specificity

Detects mouse Serpin E2/PN1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin I2, recombinant mouse (rm) Serpin C1, rmSerpin D1, and rmSerpin F2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse Serpin E2/PN1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Serpin E2/PN1 (Catalog # 2175-PI)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Serpin E2 (0.2 µg/mL, Catalog # 2175-PI) inhibition of Trypsin (0.05 µg/mL) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002 ). The Neutralization Dose (ND50) is typically 1.1 µg/mL.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin E2/PN1

Serpin E2, also known as protease nexin I or glial-derived nexin (GDN), is a member of the Serpin superfamily of the serine protease inhibitors (1). Serpin E2 is a potent inhibitor of thrombin, plasmin and plasminogen activators (2). It is differentially expressed during neuronal differentiation and is able to transform human embryonic kidney cells into neuron-like cells (3). Its over‑expression in mice leads to progressive neuronal and motor dysfunction in these animals (4). It is also over‑expressed in the majority of pancreatic carcinoma as well as gastric and colorectal cancer samples whereas it is weakly expressed in all normal pancreas and chronic pancreatitis tissue samples (5). It plays an important role in controlling male fertility because its knockout male mice show a marked impairment in fertility from the onset of sexual maturity and its abnormal expression is found in the semen of men with seminal dysfunction (6).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Rossignol, P. et al. (2004) J. Biol. Chem. 279:10346.
  3. Lin, H.J. et al. (2005) Int. J. Dev. Neurosci. 23:9.
  4. Meins, M. et al. (2001) J. Neurosci. 21:8830.
  5. Buchholz, M. et al. (2003) Cancer Res. 63:4945.
  6. Murer, V. et al. (2001) Proc. Natl. Acad. Sci. USA 98:3029.

Alternate Names

GDN, GdNPF, Proteinase Nexin 1

Entrez Gene IDs

5270 (Human); 20720 (Mouse)

Gene Symbol

SERPINE2

UniProt

Q07235

Additional Serpin E2/PN1 Products

Product Documents for Mouse Serpin E2/PN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Serpin E2/PN1 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse Serpin E2/PN1 Antibody

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