Mouse Siglec-3/CD33 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2220
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Mouse Siglec-3/CD33 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec‑3/CD33 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 is observed and less than 5% cross-reactivity with recombinant mouse (rm) Siglec-F, rmSiglec-2, rhSiglec-3, rhSiglec-5 and rhSiglec-10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Siglec‑3/CD33
Gln17-Val244
Accession # Q63994
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Siglec‑3/CD33

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-3/CD33

Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) cell-surface lectins that recognize sialic acid within specific glycan structures. Mouse Siglec-3, also known as CD33 and GP67, is expressed primarily on myeloid precursors in the bone marrow and on granulocytes in peripheral blood. Two isoforms have been reported for mouse Siglec-3 that display distinct cytoplasmic domains resulting from differential mRNA splicing.

Long Name
Sialic Acid Binding Ig-like Lectin 3
Entrez Gene IDs
945 (Human); 12489 (Mouse); 102117580 (Cynomolgus Monkey)
Alternate Names
CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67

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