Detects mouse Siglec-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 and less than 5% cross-reactivity with recombinant mouse (rm) Siglec-F, rmSiglec-2, rhSiglec‑3, rhSiglec-5, and rhSiglec-10 is observed.
Polyclonal Goat IgG
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Siglec-3/CD33 Gln17-Val244 Accession # Q63994
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Mouse Siglec‑3/CD33
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type)
cell-surface lectins that recognize sialic acid within specific glycan
structures. Mouse Siglec-3, also known as CD33 and GP67, is expressed
primarily on myeloid precursors in the bone marrow and on granulocytes
in peripheral blood. Two isoforms have been reported for mouse Siglec-3
that display distinct cytoplasmic domains resulting from differential
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