Mouse Siglec-3/CD33 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Siglec-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) Siglec-7 and rhSiglec-9 and less than 5% cross-reactivity with recombinant mouse (rm) Siglec-F, rmSiglec-2, rhSiglec‑3, rhSiglec-5, and rhSiglec-10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Siglec-3/CD33
    Gln17-Val244
    Accession # Q63994
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Siglec‑3/CD33
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-3/CD33
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) cell-surface lectins that recognize sialic acid within specific glycan structures. Mouse Siglec-3, also known as CD33 and GP67, is expressed primarily on myeloid precursors in the bone marrow and on granulocytes in peripheral blood. Two isoforms have been reported for mouse Siglec-3 that display distinct cytoplasmic domains resulting from differential mRNA splicing.
  • Long Name:
    Sialic Acid Binding Ig-like Lectin 3
  • Entrez Gene IDs:
    945 (Human); 12489 (Mouse)
  • Alternate Names:
    CD33 antigen (gp67); CD33 antigen; CD33 molecule; CD33; FLJ00391; gp67; myeloid cell surface antigen CD33; p67; sialic acid binding Ig-like lectin 3; Sialic acid-binding Ig-like lectin 3; Siglec3; Siglec-3; SIGLEC3gp67
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