Mouse SIGNR1/CD209b PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse SIGNR1 in direct ELISAs and Western blots. In Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant mouse SIGNR4.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse SIGNR1
    Accession # Q8CJ91
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of SIGNR1/CD209b in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Goat Anti-Mouse SIGNR1/CD209b PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1836P) or (B) Normal Goat IgG Phycoerythrin Control (Catalog # IC108P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIGNR1/CD209b

SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC-SIGN and DC-SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC-SIGN and DC-SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta  and gamma isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%-70% aa sequence identity with mouse DC‑SIGN, SIGNR2, 3, and 4, and 64%-68% aa sequence identity with human DC-SIGN, human L-SIGN, and rat DC-SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11-13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).

  • References:
    1. Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
    2. Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
    3. McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
    4. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
    5. Parent, S.A. et al. (2002) Gene 293:33.
    6. Kang, Y.-S. et al. (2003) Int. Immunol. 15:177.
    7. Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327.
    8. Galustian, C. et al. (2003) Int. Immunol. 16:853.
    9. Taylor, P.R. et al. (2004) J. Immunol. 172:1157.
    10. Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
    11. Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962.
    12. Lanoue, A. et al. (2004) J. Exp. Med. 200:1383.
    13. Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
    14. Nagaoka, K. et al. (2005) Int. Immunol. 17:827.
    15. Takahara, K. et al. (2003) Int. Immunol. 16:819.
  • Long Name:
    SIGN-related 1
  • Entrez Gene IDs:
    69165 (Mouse)
  • Alternate Names:
    CD209 antigen like protein B; CD209b; DC SIGN related protein 1; DC SIGNR1; OtB7
Related Research Areas
Isotype Controls
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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