Mouse SIGNR1/CD209b Antibody

Catalog # Availability Size / Price Qty
AF1836
AF1836-SP
Product Details
Citations (3)
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Mouse SIGNR1/CD209b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SIGNR1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse (rm) SIGNR3 and rmSIGNR4 is observed, and approximately 10% cross-reactivity with recombinant human DC-SIGNR and rmDC‑SIGN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SIGNR1
Ser76-Gly325
Accession # Q8CJ91
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse SIGNR1/CD209 Fc Chimera (Catalog # 1836-SR)
Adhesion Blockade
The adhesion of human ICAM-3 expressing CHO Chinese hamster ovary cells (5 x 104 cells/well) to immobilized Recombinant Mouse SIGNR1/CD209b Fc Chimera (Catalog # 1836-SR, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIGNR1/CD209b

SIGNR1 belongs to the family of C-type lectins that participate in innate immune responses by binding and clearing pathogens (1-3). SIGNR1 is one of four mouse proteins, encoded by separate genes, with significant homology to DC‑SIGN and DC‑SIGNR (4). Mouse SIGNR1 consists of a 53 amino acid (aa) cytoplasmic domain, a 20 aa transmembrane segment, and a 252 aa extracellular domain that contains a juxtamembrane neck region and a carbohydrate recognition domain (CRD) (4, 5). SIGNR1 is a 50kDa type II protein that forms high molecular weight aggregates as has been shown for DC‑SIGN and DC‑SIGNR (6, 7). The mouse SIGNR1 mRNA is extensively spliced, giving rise to multiple isoforms in addition to variability in the 5’ and 3’ untranslated regions. The alpha isoform is known as SIGNR1. The beta  and  gamma  isoforms lack the transmembrane segment, with the gamma isoform also showing a 21 aa insertion in the CRD. The δ isoform lacks almost the entire CRD (5). Functional differences between the isoforms have not been described. Within the CRD, mouse SIGNR1 shares 67%‑70% aa sequence identity with mouse DC‑SIGN, SIGNR2, 3, and 4, and 64% - 68% aa sequence identity with human DC‑SIGN, human L-SIGN, and rat DC‑SIGN. The CRD binds mannose, GlcNAc, and fucose structures on some bacterial strains, mycobacteria, and yeast (8). SIGNR1 is expressed on macrophages found in the splenic marginal zone, lymph node medulla, and peritoneum (6, 9, 10). It plays a dominant role in the clearance of circulating S. pneumoniae (11 - 13), enhances TLR4 signaling (14), and cooperates with Dectin-1 in the nonopsonic clearance of fungal pathogens (9, 15). SIGNR1 also binds mouse ICAM-2, human ICAM-2 and -3, and HIV gp120 (10).

References
  1. Marshall, A.S.J. and S. Gordon (2004) Eur. J. Immunol. 34:18.
  2. Cambi, A. and C.G. Figdor (2005) Curr. Opin. Immunol. 17:345.
  3. McGreal, E.P. et al. (2005) Curr. Opin. Immunol. 17:18.
  4. Park, C.G. et al. (2001) Int. Immunol. 13:1283.
  5. Parent, S.A. et al. (2002) Gene 293:33.
  6. Kang, Y.-S. et al. (2003) Int. Immunol. 15:177.
  7. Feinberg, H. et al. (2005) J. Biol. Chem. 280:1327.
  8. Galustian, C. et al. (2003) Int. Immunol. 16:853.
  9. Taylor, P.R. et al. (2004) J. Immunol. 172:1157.
  10. Geijtenbeek, T.B.H. et al. (2002) Blood 100:2908.
  11. Koppel, E.A. et al. (2005) Eur. J. Immunol. 35:2962.
  12. Lanoue, A. et al. (2004) J. Exp. Med. 200:1383.
  13. Kang, Y.-S. et al. (2004) Proc. Natl. Acad. Sci. 101:215.
  14. Nagaoka, K. et al. (2005) Int. Immunol. 17:827.
  15. Takahara, K. et al. (2003) Int. Immunol. 16:819.
Long Name
SIGN-related 1
Entrez Gene IDs
69165 (Mouse)
Alternate Names
CD209 antigen like protein B; CD209b; DC SIGN related protein 1; DC SIGNR1; OtB7; SIGNR1

Product Datasheets

Citations for Mouse SIGNR1/CD209b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Immunomodulatory glycan lacto-N-fucopentaose III requires clathrin-mediated endocytosis to induce alternative activation of antigen-presenting cells.
    Authors: Srivastava, Leena, Tundup, Smanla, Choi, Beak-San, Norberg, Thomas, Harn, Donald
    Infect Immun, 2014;82(5):1891-903.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Neutrophils mediate maturation and efflux of lung dendritic cells in response to Aspergillus fumigatus germ tubes.
    Authors: Park SJ, Burdick MD, Mehrad B
    Infect. Immun., 2012;80(5):1759-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Cross-linking of SIGNR1 activates JNK and induces TNF-alpha production in RAW264.7 cells that express SIGNR1.
    Authors: Numazaki M, Kato C, Kawauchi Y, Kajiwara T, Ishii M, Kojima N
    Biochem. Biophys. Res. Commun., 2009;386(1):202-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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